Comparison of MIC COL, Sensititre™ and Colistin Agar Screening Plate Methods with Broth Microdilution Reference Method for Detection of Colistin Susceptibility in Carbapenem-Resistant Gram-negative Bacteria

Çuha, Mervenur Demir; Hazırolan, Gülşen­

doi:10.36519/kd.2021.3857

Cited by 2 publications

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“…Son yıllarda, tüm dünyada olduğu gibi ülkemizde de kolistin dirençli bakteriler raporlanmıştır (5). Bu nedenle, kolistin duyarlılık testinin "Clinical and Laboratory Standards Institute (CLSI)" ve "European Committee on Antimicrobial Susceptibility Testing (EUCAST)" tarafından önerilen sıvı mikrodilüsyon yöntemi ile çalışılarak doğru sonucun raporlanması önemlidir (6). Ancak sıvı mikrodilüsyon yönteminin pratik olmamasından dolayı rutin bakteriyoloji laboratuvarlarında kolistin direncini belirlemek her zaman problem olmuştur.…”

Section: Introductionunclassified

Evaluation of Colistin Susceptibility Results in Multidrug-Resistant Gram-Negative Bacilli by Colistin Broth Disk Elution Method

Özkaçmaz

Sezgi

2022

Klimik Derg

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Objective: Determining colistin susceptibility according to the reference method is a significant problem for clinical microbiology laboratories. There is a need for a fast, easy-to-apply, and accurate method for laboratories with limited opportunities. In our study, we aimed to evaluate the applicability of the colistin broth disk elution (CBDE) test. Methods: A total of 193 Gram-negative bacteria, which were isolated from various clinical specimens in our laboratory, were included in the study. Colistin susceptibility was determined by broth microdilution as the reference method. The CBDE test was performed as previously described. For each bacterial isolate, 0, ½, 1, 2, and 4 colistin discs (10 µg; BD) were added to 5 tubes containing cation-adjusted Mueller-Hinton broth (MHB). The final colistin concentrations in the tubes were 0 (growth control), 0.5, 1, 2, and 4 µg/ml, respectively. The minimum inhibitory concentration values were determined by visual evaluation 16-20 hours after the bacterial inoculation. Results: In the Enterobacterales group, the categorical agreement was 94%, the major error was 5%, and the very major error was 0%. In the non-fermentative bacteria group, the categorical agreement was found to be 94%, the major error 1%, and the very major error 33%, respectively. Conclusion: The CBDE test is a good alternative to broth microdilution in routine microbiology laboratories for the Enterobacterales group since it provides easy-to-apply, cost-effective testing for colistin susceptibility and has a high categorical agreement compared to the reference microdilution method. Keywords: colistin, disc elution, antibiotic resistance, broth microdilution, Enterobacterales

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Section: Introductionunclassified

Evaluation of Colistin Susceptibility Results in Multidrug-Resistant Gram-Negative Bacilli by Colistin Broth Disk Elution Method

Özkaçmaz

Sezgi

2022

Klimik Derg

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“…Janet ve arkadaşlarının (17) çalışmasında, SMD referans yöntemine göre Sensititre™ yöntemi için KU oranı %96, ÇBH oranı %0 ve BH oranı %5 olarak tespit edilmiştir. Ülkemizde yapılan bir çalışmada karbapenem dirençli Gram-negatif bakterilerde kolistin için Sensititre™ panelinin referans yöntem ile KU oranı %94.9, ÇBH ve BH oranları sırası ile %7.1 ve %4.5 olarak tespit edilmiştir(18). Çalışmamızda ise, diğer çalışmalardan farklı olarak Sensititre™ yönteminde KU oranı %68, ÇBH oranı %5 ve BH oranı %27 olarak tespit edildi.…”

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Comparison of Colistin Resistance Results of Multiple Resistant Clinical Acinetobacter baumannii Isolates with broth Microdilution, Sensititre, and Two Different Automated Systems

et al. 2022

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Objectives: Although the broth microdilution method (BMD) is recommended in determining colistin resistance, the search for alternative methods continues. We aimed to determine the effectiveness in detecting colistin sensitivity by comparing the liquid microdilution method, which is the gold standard method for colistin susceptibility testing, and the Sensititre™ microdilution (Thermo Fisher Scientific, MA, USA), Phoenix™ 100 (Becton Dickinson, USA), and MicroScan WalkAway™ (Beckman Coulter, USA) automated systems, which are the commercial BMD methods. Methods: For this purpose, 100 multidrug resistant strains of Acinetobacter baumanii were tested for colistin susceptibility. Antibiotic susceptibility results were compared with broth microdilution, Sensititre™ microdilution (Thermo Fisher Scientific, MA, USA), Phoenix™ 100 (Becton Dickinson, USA), and MicroScan Walkaway™ (Beckman Coulter, USA). Results: According to the criteria set by International Organization of Standardization (ISO), categorical agreement, major error, and very large error rates were not found in acceptable performance when the Phoenix and Sensitizer microdilution methods were compared with the gold standard method BMD. In addition, we determined that the categorical agreement (97%), major error (1%), and very large error (2%) rates of the MicroScan Walkaway method were acceptable. Conclusion: Since BMD is not a practical method, its use is not preferred. Simple and accurate phenotypic detection methods to determine colistin resistance in routine microbiology laboratories have not yet been defined. Although different results were obtained in different studies, MicroScan Walkaway provided the necessary criteria for the method acceptance in our study. Keywords: colistin, Acinetobacter baumannii, broth microdilution, sensititre, automated systems

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Comparison of MIC COL, Sensititre™ and Colistin Agar Screening Plate Methods with Broth Microdilution Reference Method for Detection of Colistin Susceptibility in Carbapenem-Resistant Gram-negative Bacteria

Cited by 2 publications

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Evaluation of Colistin Susceptibility Results in Multidrug-Resistant Gram-Negative Bacilli by Colistin Broth Disk Elution Method

Evaluation of Colistin Susceptibility Results in Multidrug-Resistant Gram-Negative Bacilli by Colistin Broth Disk Elution Method

Comparison of Colistin Resistance Results of Multiple Resistant Clinical Acinetobacter baumannii Isolates with broth Microdilution, Sensititre, and Two Different Automated Systems

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