Comparison of methods to detect biosurfactant production by diverse microorganisms

Youssef, Noha H.; Duncan, Kathleen E.; Nagle, D P; Savage, Kristen N.; Knapp, R.M.; McInerney, Michael J.

doi:10.1016/j.mimet.2003.11.001

Cited by 714 publications

(534 citation statements)

References 38 publications

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“…(Cooper and Goldenbergy, 1999;Sung and Kuo, 1998). Having both polar and non-polar domains, biosurfactants are able to partition at the water/air or water/oil interfaces and thus lower the interfacial of surface tension (Youssef et al, 2004). Such properties make them good candidates for EOR (Bannat, 1995b;Bannat et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Haghighat

Sepahy

Assadi

et al. 2008

Int. J. Environ. Sci. Technol.

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ABSTARCT: Microbially produced lipopeptide have been isolated and studied for microbial enhanced oil recovery.About 60 gram positive bacteria isolated from soil contaminated with crude oil, near the crude oil storage tank in Tehran Refinery, Tehran, Iran. However, most of these studies have produced lipopeptide by one of the pure-culture microbes isolated in a laboratory. Among the isolates, heamolytic tests revealed two biosurfactant producers. The isolated strains were designated as C2, E1. By using morphological, biochemical and molecular biology tests (16 SrRNA), the strains identified as Bacillus licheniformis and Bacillus subtitlis, respectively. Emulsification activity and measurement of surface tension indicated that, the isolates were high producers of biosurfactant. The product of C2 and E1 is mainly lipopeptide. This product reduce surface tension from 65 to 30 mN/m. Emulsified activity of crude oil was 92% for C2 and 90 % in case of E1. This is the first report of indigenous Bacillus licheniformis and Bacillus subtilis from a soil contaminated with oil in an Iranian refinery with ability to produce biosurfactant.

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Section: Introductionmentioning

confidence: 99%

Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Haghighat

Sepahy

Assadi

et al. 2008

Int. J. Environ. Sci. Technol.

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“…Those cultures that gave rounded drops were scored as negative '-', indicative of the lack of biosurfactant production. 15 Oil activity assay test: The isolates present in the clearing zone on Haemolytic activity test were re-screened by hydrolyzing oil activity assay on oil agar plates. 16 Potential biosurfactant-producing strains were inoculated into the MSM (Himedia), and the cultured filter was further analyzed by improved degreasing effect assay test and emulsification activity measurement.…”

Section: Screening For Biosurfactant Producersmentioning

confidence: 99%

Characterization of Biosurfactant Produced by Bacillus Sp. Isolated From Oil Spilled Water Samples of Arabian Sea

Dhail¹

2017

IPCSE

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Pollution of Sea by various harmful hydrocarbons due to accidental leakages of oils during ship operations making it one of serious environmental issues all over the world. Biosurfactants act as one of the promising candidates for overcoming such pollutions. They are surface active molecules synthesized by microorganisms. They replace their chemically synthesized counterparts due to their eco-friendly nature on environment. In present study, we isolated some of the bacterial strains showing biosurfactant activity and further we characterized particular bacterial strain showing highest activity isolated from oil-spilled area of Arabian Sea. Various biosurfactant activity assay tests were performed for isolating the potent bacterial strain. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. Among all of the isolated strains, strain 2 identified as Bacillus sp. showed the highest biosurfactant activity. The isolated culture filtrate was found to be highly effective in microbial enhanced oil recovery (MEOR). Keywords

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“…All the fermentations were carried out using 30ml crew cap tubes with 10ml working volume and were incubated in a rotary incubator at 30ºC for 7days. The centrifuged supernatants of the cultured broth were screened for the most potential biosurfactant producer using the following multiple screening methods includes emulsification activity (Cooper and Goldenberg, 1987), lipase activity (Kiran et al, 2009) and oil displacement test (Youssef et al, 2004). Based on the promising results from these screening methods, the potential strain was selected and further studies were carried out using the potential bacterium.…”

Section: Screening Of Potential Halophilic Biosurfactant Producing Bamentioning

confidence: 99%

“…The time course on production was plotted with reference to biosurfactant production and bacterial growth. The biosurfactant activity of this potential bacterium was estimated using oil displacement test (Youssef et al, 2004) and bacterial growth was determined using hot air over (50°C) dried weight of centrifuged cell biomass at 3000 rpm for 15 min. This experiment were conducted in 250ml Erlenmeyer flask using Bushnell Haas broth as the basal medium supplemented with 1% crude oil under 100ppt salinity.…”

Section: Time Course On Biosurfactant Productionmentioning

confidence: 99%

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A glycolipid biosurfactant from a native halophilic Bacillus species of Porto Novo, India and its potential microbially enhanced oil recovery properties

2016

Int. J. Adv. Res. Biol. Sci.

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A potential biosurfactant producing halophilic bacterium was isolated from the saltpan environment of Porto Novo, Tamilnadu, India. The potential bacterium showed 69.9 % emulsification activity, 56.7 units/ml lipase activity and 22 mm oil displacement in the biosurfactant multiple screening methods. This halophilic bacterium was identified as Bacillus species using 16S rRNA molecular sequencing method. This strain showed growth dependent biosurfactant production, which was partially purified and weighed at 0.945 ±0.021 g/L on dry weight basis. The isolated biosurfactant was biochemically identified as glycolipid using biochemical methods and FT-IR functional group analysis. Further, the glycolipid surfactant showed consistent oil recovery efficiency than the tested synthetic surfactant at the temperature up to 50°C and pH ranged from 5 to 8. Based on these observations, the isolated biosurfactant using this halophilic bacterium revealed promising microbially enhanced oil recovery and bioremediation purposes at a wide range of environmental conditions.

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Comparison of methods to detect biosurfactant production by diverse microorganisms

Cited by 714 publications

References 38 publications

Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Characterization of Biosurfactant Produced by Bacillus Sp. Isolated From Oil Spilled Water Samples of Arabian Sea

A glycolipid biosurfactant from a native halophilic Bacillus species of Porto Novo, India and its potential microbially enhanced oil recovery properties

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