1988
DOI: 10.1111/j.1365-2818.1988.tb04610.x
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Comparison of methods for the estimation of the thickness of ultrathin tissue sections

Abstract: For a number of quantitative electron microscopical techniques it is relevant to obtain an estimate of the thickness t' of the section which, in general, will differ from the actual distance t between the two cuts that generate the section. To estimate t' of ultrathin sections, several techniques have been adopted in the past, both with and without the aid of the electron microscope and additional equipment, which are summarized in an appendix. In the present study five methods have been evaluated experimental… Show more

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Cited by 77 publications
(36 citation statements)
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“…where D ϭ mean diameter of varicosities, ls ϭ mean length of synapses, and t ϭ mean thickness of ultrathin sections, in this case 0.057 m, as determined using the small fold method (De Groot, 1988). Synaptic incidence was estimated from all Orximmunoreactive varicosities sampled in tissue singly labeled by immunogold-silver for Orx or dually labeled by immunoperoxidase for Orx and gold-silver for TH or GABA.…”
Section: Estimation Of Synaptic Incidencementioning
confidence: 99%
“…where D ϭ mean diameter of varicosities, ls ϭ mean length of synapses, and t ϭ mean thickness of ultrathin sections, in this case 0.057 m, as determined using the small fold method (De Groot, 1988). Synaptic incidence was estimated from all Orximmunoreactive varicosities sampled in tissue singly labeled by immunogold-silver for Orx or dually labeled by immunoperoxidase for Orx and gold-silver for TH or GABA.…”
Section: Estimation Of Synaptic Incidencementioning
confidence: 99%
“…For determination of the distance between the reference and the look-up sections, the section thickness was measured by using Small's method of minimal folds (Small, 1968) as described by Weibel (1979) and Royet (1991). This method provides accurate estimates of section thickness compared with other techniques (Calverley et al, 1988;DeGroot, 1988;Hunter and Stewart, 1989). Minimal folds lie perpendicular to the section plane and have a width that equals twice that of the section thickness.…”
Section: Quantification Of Synapsesmentioning
confidence: 99%
“…The thickness of ultrathin sections was estimated with Small's method of minimal folds (Small, 1968) as described by Weibel (1979) and Royet (1991) because this method provides accurate estimates of section thickness compared with other techniques (Calverley et al, 1988;De Groot, 1988;Hunter & Stewart, 1989). Minimal folds lie perpendicular to the section plane and have a width that equals twice that of the section thickness.…”
Section: Estimation Of Ultrathin Section Thicknessmentioning
confidence: 99%