Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci

Oliveira, Alexsandra Duarte de; Cunha, Maria de Lourdes

doi:10.1186/1756-0500-3-260

Cited by 113 publications

(114 citation statements)

References 47 publications

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“…[12,13] A total 169 isolates of Enterobacteriaceae were tested for biofilm production by tube adherence method which can be indicated for the routine detection of biofilm production because of its easy application and low-cost and because it guarantees reliable results with excellent sensitivity and specificity. [14,15] In our study, 59.2% of the tested organisms have shown the potential to make biofilms in comparison to similar study [16] which reported 54%.…”

Section: Discussionmentioning

confidence: 47%

Determination of correlation between biofilm and extended spectrum β lactamases producers of Enterobacteriaceae

Subramanian¹,

Umadevi²,

Kumar³

et al. 2012

Scho Res J

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Background: Biofilm is a complex aggregate of microorganisms in which cells adhere to each other to a surface in a self-produced matrix of extra-cellular polymeric substance. Antibiotic resistance of bacteria in the biofilm mode of growth contributes to the chronicity of infections. Aims: This study was aimed to find out the prevalence of biofilm producers among the microorganisms isolated from our setup and to find out their antimicrobial susceptibility pattern with special reference to Extended Spectrum β-lactamase (ESBL) and AmpC producers. Settings and Design: It is a prospective, analytic study. Materials and Methods: The study included 169; non-repeat, clinical isolates of Enterobacteriaceae collected over a period of 6 months. All isolates were tested for biofilm production by tube adherence methods. Any bacterial species which showed resistance to any of third generation cephalosporin was tested for ESBL production and AmpC production. Statistical Analysis: Statistical analysis was carried out by taking percentage and simple ratios. Results: Among 169 isolates, 100 (59.2%) were biofilm producers. In our study, 44 (26%) and 43 (25%) isolates were ESBL and AmpC producers respectively. Of 44 ESBL producers, 42 (95.4%) and of 43 AmpC producers 39 (90.7%) were biofilm producers. Conclusions: Among 169 isolates, 26% and 25% were ESBL and AmpC producers. Of which, more than 90% were biofilm producers, which showed high resistance to commonly used antibiotics. Disabling biofilm resistance may enhance the ability of existing antibiotics to clear infections involving biofilms that are refractory to current treatments.

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Section: Discussionmentioning

confidence: 47%

Determination of correlation between biofilm and extended spectrum β lactamases producers of Enterobacteriaceae

Subramanian¹,

Umadevi²,

Kumar³

et al. 2012

Scho Res J

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“…The CRA method due to its easiness and good sensitivity has been widely used for phenotypic detection of biofilm production in different bacteria (21). In the present study, 54.4% and 70.8% of S. aureus and S. epidermidis isolates were recognized as biofilm producer by CRA method, respectively.…”

Section: Discussionmentioning

confidence: 67%

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Zalipour

Ebrahim‐Saraie

Sarvari

et al. 2016

Jundishapur J Microbiol

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Background: Biofilm formation capacity is recognized as an important virulence factor in staphylococci that makes the organisms more resistant to antibiotics and host defenses. Objectives: This study aimed to determine the biofilm producing ability and presence of icaA/D genes in staphylococcal isolates obtained from different clinical specimens. Methods: This cross-sectional study was performed on a total of 151 staphylococcal isolates (79 Staphylococcus aureus and 72 S. epidermidis) obtained from different clinical specimens from February to August 2013 in Shiraz, Southwest of Iran. Slime production ability was evaluated using the both phenotypic (by cultivation of staphylococcal isolates on Congo red agar (CRA)) and genotypic (detection of the presence of icaA/D genes by PCR) methods. Results: Overall, of the 79 S. aureus isolates tested with CRA method, 64.7% of methicillin-resistant S. aureus (MRSA) isolates, and 46.7% of methicillin-sensitive S. aureus (MSSA) isolates were able to produce biofilm. The relative frequency of biofilm producing S. epidermidis isolates was 70.8% that was significantly higher than that of S. aureus isolates. The most common source of biofilm producing isolates in both S. aureus and S. epidermidis isolates was endotracheal tube (ETT) with 100% biofilm formation. Moreover, the presence of icaA/D genes was detected in 63.3% and 81.9% of S. aureus and S. epidermidis isolates, respectively. Conclusions: The remarkable rates of biofilm production ability among clinically isolated staphylococci emphasize the necessity of more effective infections control policies to prevent biofilm formation on medical devices and hospital environmental surfaces.

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“…Oliveira and Cucha confirmed the relatively high efficiency of the microplate method [21]. Analysis of 100 CoNS strains isolated from clinical specimens obtained from newborns and tested with the method described by Christensen et al , showed that 46% of isolated were characterized by strong phenotypic capacity for biofilm production, while 35% of them were weak biofilm producers.…”

Section: Resultsmentioning

confidence: 90%

“…Medical implants contaminated by biofilm-forming bacteria may lead to the development of inflammatory foci where implant removal is frequently the only effective treatment of such infections [19,20,21]. Since Staphylococci are part of the resident microbiological flora of the skin the presence of the biofilm-forming strains among them may be associated with an increased risk of transmission of virulent biofilm-forming strains in the hospital environment.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation and Antimicrobial Susceptibility of Staphylococcus epidermidis Strains from a Hospital Environment

Wojtyczka

Orlewska

Kępa

et al. 2014

IJERPH

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The hospital environment microflora comprise a wide variety of microorganisms which are more or less pathogenic and where staphylococci are one of the most common types. The aim of the presented study was to evaluate the prevalence of the biofilm forming coagulase-negative staphylococci (CoNS) in a hospital environment as a risk factor for nosocomial infections. Among 122 isolated and tested strains of CoNS the most frequent were: S. epidermidis—32 strains, S. haemolyticus—31 strains, S. capitis subsp. capitis—21 strains, S. hominis—11 strains, S. cohnii subsp. cohnii—nine strains. In case of CoNS, the main molecule responsible for intercellular adhesion is a polysaccharide intercellular adhesin (PIA), encoded on the ica gene operon. The analysis revealed the presence of the icaADBC operon genes in 46.88% of S. epidermidis isolates. IcaA and icaD were present in 34.38% and 28.13% of strains respectively while IcaC gene was present in 37.50% of strains. IcaB gene was found in 21.88% of S. epidermidis strains. In 15 (63%) strains all icaADBC operon genes were observed. The assessment of antibacterial drugs susceptibility demonstrated that analyzed CoNS strains were highly resistant to macrolides and lincosamides and more sensitive to rifampicin and linezolid. Our data indicates that the hospital environment can be colonized by biofilm forming coagulase-negative staphylococci and transmission of these strains can cause an increased risk of serious nosocomial infections.

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Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci

Cited by 113 publications

References 47 publications

Determination of correlation between biofilm and extended spectrum β lactamases producers of Enterobacteriaceae

Determination of correlation between biofilm and extended spectrum β lactamases producers of Enterobacteriaceae

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Biofilm Formation and Antimicrobial Susceptibility of Staphylococcus epidermidis Strains from a Hospital Environment

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