Comparison of methods for isolation of Mycobacterium avium complex DNA for use in PCR and RAPD fingerprinting

Via, Laura E.; Falkinham, Joseph O.

doi:10.1016/0167-7012(94)00045-9

Cited by 22 publications

(18 citation statements)

References 14 publications

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“…8 AE 1 . 4 mg g À1 from M. ulcerans cells harvested from L-J slants is comparable to other DNA extraction methods for slow-growing mycobacteria (Belisle & Sonnenberg, 1998;Santos et al, 1992;Via & Falkinham, 1996;Zhang & Ishaque, 1997). The mean yield of genomic DNA for ten different M. ulcerans strains was 3 .…”

Section: Optimization Of Cell Lysis and Dna Extractionmentioning

confidence: 54%

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One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish

Kotłowski

Martin

Ablordey

et al. 2004

Journal of Medical Microbiology

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The purpose of this study was to develop a simple procedure for cell lysis and DNA extraction for direct detection of Mycobacterium ulcerans in aquatic insects, gills and intestinal contents of fish, molluscs and human tissue samples using a nested PCR method specific for the insertion sequence IS2404. The simultaneous action of sodium N-lauroyl sarcosine, guanidinium isothiocyanate, chloroform and Tris-saturated phenol on mycobacteria, followed by a DNA purification method using mini-columns fitted with silica-cellulose membranes was successfully employed to extract DNA from cultured bacteria, environmental and human tissue samples. All specimens were collected from Buruli ulcer endemic regions. M. ulcerans DNA was detected in 11 of 57 aquatic insects, one of six molluscs and three of 15 fish, supporting the hypothesis that the fauna of major Buruli ulcer endemic foci in swampy terrain of tropical and subtropical regions can be a source of M. ulcerans infection.

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Section: Optimization Of Cell Lysis and Dna Extractionmentioning

confidence: 54%

“…Mechanical protocols for disruption of mycobacteria using MiniBeadBeaters are very efficient for pure cultures (Via & Falkinham, 1996;Zhang & Ishaque, 1997, Kim et al, 2001) but need to be modified, depending on the chemical composition and rheological properties of samples.…”

Section: Optimization Of Cell Lysis and Dna Extractionmentioning

confidence: 99%

One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish

Kotłowski

Martin

Ablordey

et al. 2004

Journal of Medical Microbiology

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“…In most experiments, the same zirconia/silica beads were used in both devices. BioSpec protocols reported in the literature use either glass beads or zirconia/silica beads for lysis of mycobacteria (22,33,35). Based on our results (Fig.…”

Section: Discussionmentioning

confidence: 76%

“…The processing time per sample for the OmniLyse device (1.5 min without rest), however, was significantly less than for the BioSpec BeadBeater devices (three 1-min pulses with 30-s rests between pulses). The BioSpec processing time used in the present study was based on standard published protocols (10,13,33,35), and appears to be the time required using this system for effective lysis of Bacillus spores and mycobacterial cells. In most experiments, the same zirconia/silica beads were used in both devices.…”

Section: Discussionmentioning

confidence: 99%

Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device

et al. 2011

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Molecular detection of microorganisms requires microbial cell disruption to release nucleic acids. Sensitive detection of thick-walled microorganisms such as Bacillus spores and Mycobacterium cells typically necessitates mechanical disruption through bead beating or sonication, using benchtop instruments that require line power. Miniaturized, low-power, battery-operated devices are needed to facilitate mechanical pathogen disruption for nucleic acid testing at the point of care and in field settings. We assessed the lysis efficiency of a very small disposable bead blender called OmniLyse relative to the industry standard benchtop Biospec Mini-BeadBeater. The OmniLyse weighs approximately 3 g, at a size of approximately 1.1 cm 3 without the battery pack. Both instruments were used to mechanically lyse Bacillus subtilis spores and Mycobacterium bovis BCG cells. The relative lysis efficiency was assessed through real-time PCR. Cycle threshold (C T ) values obtained at all microbial cell concentrations were similar between the two devices, indicating that the lysis efficiencies of the OmniLyse and the BioSpec Mini-BeadBeater were comparable. As an internal control, genomic DNA from a different organism was spiked at a constant concentration into each sample upstream of lysis. The C T values for PCR amplification of lysed samples using primers specific to this internal control were comparable between the two devices, indicating negligible PCR inhibition or other secondary effects. Overall, the OmniLyse device was found to effectively lyse tough-walled organisms in a very small, disposable, batteryoperated format, which is expected to facilitate sensitive point-of-care nucleic acid testing.

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“…Preliminary studies of the selection of disruption technique would be conducted for the efficient extraction of products like DNA [6], RNA [7], proteins [8], and other substances [9][10][11]. Table 1 shows the comparison of disruption techniques conducted by some researchers for the selection of a technique for product recovery.…”

Section: Introductionmentioning

confidence: 99%

The performance of a glass bead shaking technique for the disruption of Escherichia coli cells

Ramanan

Ling

Ariff

2008

Biotechnol Bioproc E

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The efficacy of a simple laboratory method for cell disruption based on the shaking of glass beads on a rotary shaker was assessed in this study, via measurements of the release of total protein and interferon-α2b from bK=ÅçäáK The optimum conditions for cell disruption were detected after 30 min of shaking in Tris-HCl buffer (pH 8) at 300 rpm with 1.5 g of glass beads (diameter: 0.5 mm) per mL of cell suspension volume. Three test runs were conducted under the above conditions and the maximum average protein release values were determined as 3.048, 3.564, and 3.015 mg/mL, respectively. The amount of protein release was comparable to the amount of protein release in ultrasonication and glass bead vortexing procedures. The amount of interferon-α2b release in the ultrasonication, glass bead vortexing, and glass bead shaking trials were 240, 172, and 201 ng/mL, respectively. This method was shown to process between 1 and 10 mL of sample volume in a 50 mL Falcon tube without a great deal of deviation, and was able to handle in excess of 60 samples simultaneously. © KSBB hÉóïçêÇëW=ÅÉää=ÇáëêìéíáçåI=ÇçïåëíêÉ~ã=éêçÅÉëëáåÖI=E. coliI=Öä~ëë=ÄÉ~ÇëI=ëÜ~âÉêI=áåíÉêÑÉêçåJαOÄ= = = = =

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Comparison of methods for isolation of Mycobacterium avium complex DNA for use in PCR and RAPD fingerprinting

Cited by 22 publications

References 14 publications

One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish

One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish

Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device

The performance of a glass bead shaking technique for the disruption of Escherichia coli cells

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