Comparison of Methods for Detection of Escherichia coli O157:H7 in Ground Beef and Radish Sprouts

Lee, Jae‐Hoon; Hyeon, Ji-Yeon; Heo, Seok; Hwang, Inchan; Kwak, Hyo‐Sun; Choi, In‐Soo; Park, Chankyu; Seo, Kun‐Ho

doi:10.5851/kosfa.2010.30.2.179

Korean Journal for Food Science of Animal Resources

2010

DOI: 10.5851/kosfa.2010.30.2.179

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Comparison of Methods for Detection of Escherichia coli O157:H7 in Ground Beef and Radish Sprouts

Jae‐Hoon Lee¹,

Ji-Yeon Hyeon²,

Seok Heo³

et al.

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2021

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Cited by 2 publications

References 24 publications

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Evaluation of Ceftazidime as an Antibiotic Supplement in Mannitol–Yolk–Polymyxin B Agar Used for Enumeration of Bacillus cereus in Ready-to-Eat Vegetables

Chon

Seo

2021

Journal of Food Protection

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Bacillus cereus, which causes foodborne disease, is detected using selective media. However, competing flora is the most common factor preventing the correct enumeration of B. cereus on selective agars. In this study, we aimed to improve the selectivity of mannitol-yolk-polymyxin B agar (MYPA) and its modified version containing trimethoprim (mMYPA) developed in our previous study by supplementation with ceftazidime (16 μg/mL). Ceftazidime-supplemented MYPA (C-MYPA16) and mMYPA (C-mMYPA16) were evaluated for bacteria recoverability and selectivity using three types of ready-to-eat vegetables. Four B. cereus and one B. thuringiensis strains were mixed and artificially inoculated into vegetable salad, radish sprouts, and sprout mix, and then recovered using MYPA, mMYPA, C-MYPA16, and C-mMYPA16. In all tested vegetables, mMYPA, C-MYPA16, and C-mMYPA16 exhibited similar recoverability of B. cereus / thuringiensis ( p > 0.05), whereas MYPA showed undistinguishable colonies in case of radish sprouts and sprout mix. At the same time, C-mMYPA16 provided the best selectivity compared with the other agars because it eliminated most of competing flora in the tested vegetables, especially in sprouts, without negatively affecting the recovery of B. cereus / thuringiensis . Our results indicate that the supplementation of mMYPA with ceftazidime may improve medium selectivity for B. cereus / thuringiensis in food testing.

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Evaluation of Ceftazidime as an Antibiotic Supplement in Mannitol–Yolk–Polymyxin B Agar Used for Enumeration of Bacillus cereus in Ready-to-Eat Vegetables

Chon

Seo

2021

Journal of Food Protection

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Detection of Campylobacter jejuni from Fresh Produce: Comparison of Culture- and PCR-based Techniques, and Metagenomic Approach for Analyses of the Microbiome before and after Enrichment

Chon

Jung

Ahn

et al. 2021

Journal of Food Protection

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In this study, we compared the efficiency of culture-based methods with or without membrane filtration, real-time PCR and digital droplet PCR (ddPCR) for the detection of Campylobacter in fresh produce. Alfalfa sprouts, clover sprouts, coleslaw, and lettuce salad spiked with Campylobacter jejuni ( C. jejuni ) were enriched in Bolton broth for 48 h and enrichment cultures were either directly inoculated onto modified charcoal-cefoperazone-deoxycholate agar or applied on membrane filters placed on the surface of plating media. In parallel, 2 mL Bolton broth cultures were taken to extract DNA for real-time and ddPCR assays and bacterial community analysis. There was no significant difference ( p > 0.05) in the detection efficiency of positive Campylobacter isolates from coleslaw and lettuce salad using four detection methods. However, for sprout samples, the detection efficiency of the culture method was significantly ( p < 0.05) lower than those of the two PCR assays and the filtration method. The analysis also revealed the presence of Pseudomonas and Acinetobacter as the most prevalent competing microbiota in enriched culture and only Acinetobacter on agar plates in the selective culture step.

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Comparison of Methods for Detection of Escherichia coli O157:H7 in Ground Beef and Radish Sprouts

Cited by 2 publications

References 24 publications

Evaluation of Ceftazidime as an Antibiotic Supplement in Mannitol–Yolk–Polymyxin B Agar Used for Enumeration of Bacillus cereus in Ready-to-Eat Vegetables

Evaluation of Ceftazidime as an Antibiotic Supplement in Mannitol–Yolk–Polymyxin B Agar Used for Enumeration of Bacillus cereus in Ready-to-Eat Vegetables

Detection of Campylobacter jejuni from Fresh Produce: Comparison of Culture- and PCR-based Techniques, and Metagenomic Approach for Analyses of the Microbiome before and after Enrichment

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