Comparison of meshes, gels and ceramic for cartilage tissue engineering in vitro

Tellisi, Nazzar; Ashammakhi, Nureddin

doi:10.1007/s00238-007-0189-8

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…In Study 1, we investigated the effect of BG 13-93 on a tissue-engineered cartilage layer when it was used as a subchondral substrate. Histologic analysis of the tissueengineered layer confirmed a homogenous distribution of GAG in constructs from the center to the periphery with BG 13-93 constructs, similar to previous work with Bioglass 1 45S5 [41]. BG 13-93 was associated with higher GAG release in the middle of the culture period, which coincided with TGF-b3 removal from the medium.…”

Section: Discussionsupporting

confidence: 81%

“…However, these findings have not been found with other BGs. Tellisi and Ashammakhi [41] investigated the effect of Bioglass 1 45S5 on osteoarthritic chondrocytes and found substantial staining of collagen II with maintenance of the chondrocytic phenotype similar to freshly isolated articular chondrocytes. Loty et al [31] reported a BG containing apatite and wollastionite caused chondrocytes to produce an extracellular matrix with high collagen II and proteoglycan.…”

Section: Discussionmentioning

confidence: 99%

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Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Jayabalan

Tan

Rahaman

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Replacement of diseased areas of the joint with tissue-engineered osteochondral grafts has shown potential in the treatment of osteoarthritis. Bioactive glasses are candidates for the osseous analog of these grafts. Questions/purposes (1) Does Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate improve collagen and glycosaminoglycan production in a tissue-engineered cartilage layer? (2) Does BG 13-93 as a culture medium supplement increase the collagen and glycosaminoglycan production and improve the mechanical properties in a tissue-engineered cartilage layer?Methods In Study 1, bioactive glass samples (n = 4) were attached to a chondrocyte-seeded agarose layer to form an osteochondral construct, cultured for 6 weeks, and compared to controls. In Study 2, bioactive glass samples (n = 5) were cocultured with cell-seeded agarose for 6 weeks. The cell-seeded agarose layer was exposed to BG 13-93 either continuously or for the first or last 2 weeks in culture or had no exposure. Results Osteochondral constructs with a BG 13-93 base had improved glycosaminoglycan deposition but less collagen II content. Agarose scaffolds that had a temporal exposure to BG 13-93 within the culture medium had improved mechanical and biochemical properties compared to continuous or no exposure. Conclusions When used as a subchondral substrate, BG 13-93 did not improve biochemical properties compared to controls. However, as a culture medium supplement, BG 13-93 improved the biochemical and mechanical properties of a tissue-engineered cartilage layer. Clinical Relevance BG 13-93 may not be suitable in osteochondral constructs but could have potential as a medium supplement for neocartilage formation.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Jayabalan

Tan

Rahaman

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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“…It has also exhibited potential in facilitating soft tissue bonding [9,38,39]. On soft tissue engineering applications, bioactive glass demonstrated promising outcomes, including in implant-soft tissue integration, vascularization, control of degradation rate, and promoting chondrogenesis [37,40,41,42,43,44,45,46,47]. In fibrocartilage replacement, bioactive glass was applied as a coating and was reported to be capable of stimulating bonding between implant and bone tissue [15,43,48].…”

Section: Introductionmentioning

confidence: 99%

Mechanical and Cytocompatibility Evaluation of UHMWPE/PCL/Bioglass® Fibrous Composite for Acetabular Labrum Implant

2019

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In this study, a fibrous composite was developed as synthetic graft for labral reconstruction treatment, comprised of ultra-high molecular weight polyethylene (UHMWPE) fabric, ultrafine fibre of polycaprolactone (PCL), and 45S5 Bioglass®. This experiment aimed to examine the mechanical performance and cytocompatibility of the composite. Electrospinning and a slurry dipping technique were applied for composite fabrication. To assess the mechanical performance of UHMWPE, tensile cyclic loading test was carried out. Meanwhile, cytocompatibility of the composite on fibroblastic cells was examined through a viability assay, as well as SEM images to observe cell attachment and proliferation. The mechanical test showed that the UHMWPE fabric had a mean displacement of 1.038 mm after 600 cycles, approximately 4.5 times greater resistance compared to that of natural labrum, based on data obtained from literature. A viability assay demonstrated the predominant occupation of live cells on the material surface, suggesting that the composite was able to provide a viable environment for cell growth. Meanwhile, SEM images exhibited cell adhesion and the formation of cell colonies on the material surface. These results indicated that the UHMWPE/PCL/Bioglass® composite could be a promising material for labrum implants.

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“…Composites such as PEO/chitosan and PLLA/PCL composites were also achieved to enhance the biocompatibility for cartilage tissue engineering applications [116,122]. Using the similar method, scaffolds composed of PLGA and nano-sized HA can be produced and the constructs were shown to be [145]. However, when assessing the phenotype of chondrocytes on the PGA scaffolds, it was found that the overall cell morphology flattens with increased culture period, indicating signs of differentiation.…”

Section: Microspheresmentioning

confidence: 99%

Development of silk fibroin tissue engineering scaffolds via indirect additive manufacturing for cartilage regeneration

Liu¹

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Comparison of meshes, gels and ceramic for cartilage tissue engineering in vitro

Cited by 7 publications

References 48 publications

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Mechanical and Cytocompatibility Evaluation of UHMWPE/PCL/Bioglass® Fibrous Composite for Acetabular Labrum Implant

Development of silk fibroin tissue engineering scaffolds via indirect additive manufacturing for cartilage regeneration

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