Comparison of mesenchymal stem cell sheets and chondrocyte sheets in a rabbit growth plate injury model

Abstract

Background/aim: The treatment of posttraumatic deformities and differences in length between the extremities resulting from physeal injury remains controversial. The aims of this study were to compare the efficacy of tissue-engineered, monolayer, and allogeneic mesenchymal stem cell sheets and chondrocyte sheets for physeal arrest treatment and to investigate cell sheet technology as a novel method for cell transplantation in physeal cartilage repair. Materials and methods: A proximal tibial physeal injury was induced in New Zealand rabbits. Allogeneic mesenchymal stem cells (MSCs) and chondrocytes were cultured in temperature-responsive culture dishes and applied to the iatrogenic partial growth plate defects in single-sheet grafts (cell sheets). Treatment efficacy was determined using radiological measurements, as well as histological and immunohistochemical staining. Results: Treatment with MSCs and chondrocytes prevented endochondral ossification in the physeal plate, and bone growth resumed after treatment in both the MSC and chondrocyte cell groups. We found significant differences in radiological evaluations between preand posttreatment measurements in both MSC and chondrocyte groups. Transplanted cells were observed in the damaged area in both of the groups, which differentiated in the direction of growth plate cartilage. Conclusion: Our results support the hypothesis that MSC or chondrocyte transplantation using the cell-sheet technique described in the present study aids in the regeneration of cartilage tissue during physeal arrest after growth plate damage.

3D-printed PCL scaffolds with anatomy-inspired bionic stratified structures for the treatment of growth plate injuries

“…Bioinspired scaffolds for the implantation of growth plate defects were designed to be 2 mm thick per previous studies [ 25 , 30 , 31 ]. During 3D printing, the layer height was set as 0.2 mm, and each bionic scaffold had 10 layers.…”

Tissue engineering in growth plate cartilage regeneration: Mechanisms to therapeutic strategies

“…And the cell sheets developed in the present study facilitated growth plate cartilage regeneration and sufficiently prevented the development of a bone bridge. 188 It is worth mentioning that cartilage cell sheets made from a thermal responsive culture dish (TRCD) combined with traditional bone marrow stimulation were tested in eight patients. Cell sheet transplantation has been shown to be safe and effective in cartilage regeneration in defect sites.…”

Roles of Chondroitin Sulfate Proteoglycans as Regulators of Skeletal Development

“…Examples include: i) use of various cell types, i.e., bone marrow mesenchymal stem cells (BMSCs) or chondrocytes; ii) and various growth factors (TGFβ, IGF1, and FGF2) (Chen et al, 2020;Wei et al, 2020); iii) as well as different scaffolds composed of natural or synthetic material (Abdollahiyan et al, 2020), all with varying results. Mesenchymal stem cells (MSCs) are widely used in engineering of cartilage due to their capability for self-renewal and their ability to secrete multiple growth factors (Gultekin et al, 2020). As well, there is an influx of MSCs to the injured growth plate site, suggesting that MSCs are naturally vital to the repair process (Zhou et al, 2004).…”