Comparison of media for the detection of bifidobacteria, lactobacilli and total anaerobes from faecal samples

Hartemink, R.; Rombouts, F.M.

doi:10.1016/s0167-7012(99)00031-7

Cited by 128 publications

(90 citation statements)

References 33 publications

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“…Growth conditions were aerobic at 30°C for 48 h and anaerobic at 37°C for 48-72 h for selection of mesophilic lactobacilli and bifidobacteria, respectively. For each faecal sample, 10 random colonies were picked from appropriate plate dilutions for analysis in vitro [13,15,16] Cell morphology and cell motility of selected colonies were evaluated for genus confirmation [17].…”

Section: Isolation and Growth Of Lactobacilli And Bifidobacteriamentioning

confidence: 99%

Immune-modulating effects in mouse dendritic cells of lactobacilli and bifidobacteria isolated from individuals following omnivorous, vegetarian and vegan diets

et al. 2017

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Abbreviations used in this paper: DCs, dendritic cells; TPY, trypticase-phytone-yeast extract; MRS, de Man, Rogosa and Sharpe medium; GM-CSF, granulocyte-macrophage colony-stimulating factor; SCFAs, short-chain fatty acids; LAB, lactic acid bacteria; CTLA-4, cytotoxic T-lymphocyte antigen 4. *Manuscript Click here to view linked References AbstractLactobacilli and bifidobacteria play a primary role in modulation of gut immunity. By considering that microbiota composition depends on various factors, including diet, we asked whether functional differences could characterize faecal populations of lactobacilli and bifidobacteria isolated from individuals with different dietary habits. 155 healthy volunteers who followed omnivorous, ovo-lacto-vegetarian or vegan diets were recruited at four Italian centres (Turin, Parma, Bologna and Bari). Faecal samples were collected; lactobacilli and bifidobacteria were isolated on selective media and their immunomodulatory activity was tested in mouse dendritic cells (DCs). Pre-incubation with lactobacilli increased LPS-induced expression of the maturation markers CD80 and CD86, whereas pre-incubation with bifidobacteria decreased such expression.Analysis of the cytokine profile indicated that strains of both genera induced down-regulation of IL-12 and up-regulation of IL-10, whereas expression of TNF-was not modulated. Notably, analysis of anti-inflammatory potential (IL-10/IL-12 ratio) showed that lactobacilli evoked a greater antiinflammatory effect than did bifidobacteria in the omnivorous group (P<0.05). We also found significantly reduced anti-inflammatory potential in the bacterial strains volunteers in comparison with those from the cognate groups from the other centres. In conclusion, lactobacilli and bifidobacteria showed a genus-specific ability of modulating in vitro innate immunity associated with a specific dietary habit. Furthermore, the geographical area had a significant impact on the anti-inflammatory potential of some components of faecal microbiota.

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Section: Isolation and Growth Of Lactobacilli And Bifidobacteriamentioning

confidence: 99%

Immune-modulating effects in mouse dendritic cells of lactobacilli and bifidobacteria isolated from individuals following omnivorous, vegetarian and vegan diets

et al. 2017

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“…In particular, total aerobes, total anaerobe bacteria, Enterobactericeae, coliforms, Lactobacillus spp. and Salmonella were enumerated using nutrient agar, violet red bile glucose agar, violet red bile lactose agar, Rogosa agar and Brillant green agar according to Hartemink and Rombouts (1999). Plates were then incubated at 37 °C for 24 to 72 h aerobically and colonies were counted.…”

Section: Sampling and Measurementsmentioning

confidence: 99%

“…Anaerobic incubation was achieved using appropriate catalysts (Anaerocult A, Merck, Darmstadt, Germany) in sealed anaerobic jars (Oxoid, Basingstoke, UK). Results were expressed as log 10 colony-forming units per gram of digesta (Hartemink and Rombouts 1999).…”

Section: Sampling and Measurementsmentioning

confidence: 99%

Influence of dietary organic acid blend supplementation and interaction with delayed feed access after hatch on broiler growth performance and intestinal health

Cengiz¹,

Köksal²,

Tatlı³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:A trial was conducted to investigate the effects of a dietary organic acid blend for a period of 35 days on the growth performance, intestinal histomorphology and microflora of male broiler chicks with delayed access to feed. One hundred and ninety two one day old broiler chicks (ROSS 308) were randomly distributed into 4 groups housed in four replicate pens with 12 birds in each. A 2 × 2 factorial design was implemented. Four experimental groups were formed by two levels of dietary organic acid blend supplementation (Control and Fysal Dry ® ) and two periods of delayed feed access (0 and 36 h). At 36 h after hatching body weight and body weight change of chicks were significantly (P < 0.001) lower than groups fed immediately after hatching. Delayed feed access had an adverse impact (P < 0.001) on the body weight and feed consumption of broiler chickens on days 14 and 28. Between the days 28 and 35 of the feeding period, these differences disappeared. The relative weight of gizzard (P < 0.05), pancreas (P < 0.01) on day 6 and intestine (P < 0.05) on day 10, and gizzard (P < 0.01) on day 10 were reduced in birds subjected to delayed feed access. Dietary organic acid blend inclusion increased villus length (P < 0.001), whereas delayed feed access decreased villus length (P < 0.05) and increased the incidence of epithelial degeneration and basal membrane separation of the propria mucosa of villus in the jejenum. A significant decrease in Enterobacteriaceae count (P < 0.01) was noted in organic acid blend supplemented groups on day 25. Pectoral muscle malondialdehyde levels were decreased (P < 0.01) with dietary organic acid blend supplementation at day 10. Delayed feed access significantly increased (P < 0.05) the heterophil:lymphocyte ratio at day 6. Overall, dietary organic acid blend supplementation helped broiler chicks to develop a healthier intestinal microflora and this may, in turn, inhibit the delayed feed access-induced increase in malondialdehyde in the early growing period. However, the inclusion of organic acid blend to broiler diets may not be a protective management practice in preventing delayed feed access-related growth depression of broiler chickens.

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“…To evaluate products containing probiotic bifidobacteria, an appropriate method to analyze the exact number of viable bifidobacteria is necessary. However as shown in previous reports (3,8,18), the counts are affected by the enumeration conditions, especially the diluent.…”

Section: Discussionmentioning

confidence: 55%

“…Enumeration by the culture method is a relatively easy technique and because of this its applicability to qualitative investigations (8,18) will continue to be important for many years to come. Recently, Abe et al (3) reported that in the plate counting method, the suspension-dilution medium and plating medium affected the viable counts of various Bifidobacterium species, and that the viable counts differed greatly depending on the diluents or plating media used in the plate count.…”

Section: Introductionmentioning

confidence: 99%

Effect of Enumeration Method on <i>Bifidobacterium</i> Cell Counts in Commercial Powder Products

Muto

Abe

Yaeshima

et al. 2010

Bioscience Microflora

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In this study, the effects of enumeration methods on viable bifidobacterial counts in powder products were examined to determine the most appropriate enumeration conditions. The bifidobacterial counts of nine commercial powder products containing Bifidobacterium species were determined by a pour plate method using different diluents including Mitsuoka's buffer and Ringer's solution. Mitsuoka's buffer gave significantly higher bifidobacterial counts than Ringer's solution when used as diluent for eight of nine commercial powder products containing various Bifidobacterium species. The counts obtained with Ringer's solution were on average 51.6% of those obtained using Mitsuoka's buffer. Addition of Tween 80 or phosphate buffer to Ringer's solution increased the bifidobacterial counts. Also, the diluent had a significantly greater impact in the suspension step than in the subsequent dilution step. In conclusion, the diluent has great effect on the enumeration of bifidobacteria when using the plate count method. Also, the composition of diluents influences bifidobacterial counts.

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Comparison of media for the detection of bifidobacteria, lactobacilli and total anaerobes from faecal samples

Cited by 128 publications

References 33 publications

Immune-modulating effects in mouse dendritic cells of lactobacilli and bifidobacteria isolated from individuals following omnivorous, vegetarian and vegan diets

Immune-modulating effects in mouse dendritic cells of lactobacilli and bifidobacteria isolated from individuals following omnivorous, vegetarian and vegan diets

Influence of dietary organic acid blend supplementation and interaction with delayed feed access after hatch on broiler growth performance and intestinal health

Effect of Enumeration Method on <i>Bifidobacterium</i> Cell Counts in Commercial Powder Products

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