Comparison of media for selection and enumeration of mouse fecal flora populations

Nelson, Garret B.; George, S. Elizabeth

doi:10.1016/0167-7012(95)00004-5

Cited by 38 publications

(28 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A loop full of bacterial culture was thoroughly mixed with the hydrogen peroxide on the slide. The mixture was observed for the production of gas bubbles (Nelson and George, 1995).…”

Section: Catalase Testmentioning

confidence: 99%

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Makete

Aiyegoro

Thantsha

2016

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

“…A loop full of bacterial culture was thoroughly mixed with the hydrogen peroxide on the slide. The mixture was observed for the production of gas bubbles (Nelson and George, 1995).…”

Section: Catalase Testmentioning

confidence: 99%

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Makete

Aiyegoro

Thantsha

2016

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

“…Secondly, the accuracy of this method is dependent on selectivity of the medium. Often media are not truly speci¢c and growth of unwanted bacteria a¡ects the true counts, or media are so speci¢c that only certain strains of a group grow on the plates [9]. This necessitates detailed phenotypic characterization which may be laborious and inaccurate.…”

Section: Introductionmentioning

confidence: 99%

Comparison of viable cell counts and fluorescence in situ hybridization using specific rRNA-based probes for the quantification of human fecal bacteria

Harmsen¹

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

Conventional cultivation and fluorescence in situ hybridization (FISH) using 16S rRNA-based probes were compared for the enumeration of human colonic bacteria. Groups of common intestinal anaerobic bacteria were enumerated in slurries prepared from fecal samples of three healthy volunteers. To introduce variation between the samples, they were incubated for 48 h in batch culture (anaerobic) fermenters at 37³C, and pure cultures of Bifidobacterium infantis, Clostridium perfringens, or Lactobacillus acidophilus were added. Samples were taken from the fermenters at different times. Total anaerobes, bifidobacteria, bacteroides, clostridia, and lactobacilli were enumerated by both plating and FISH. The results showed that plate counts of total anaerobes, bifidobacteria, lactobacilli and bacteroides were approximately ten-fold lower than the corresponding FISH counts. Numbers of clostridia were higher using the plating method, probably because the clostridia probe used in FISH analyses was designed to only detect part of the genus Clostridium. The introduced variation in the methods could be detected by both methods and was comparable. ß

show abstract

“…However, the microbiota present in feces does not necessarily reflect the specific features of that in the upper gastrointestinal tract, and different species may dominate in different parts of the gut (20,36). Moreover, it is often difficult to characterize the bacterial diversity in fecal samples because of the poor or too high selectivity of selective agar media (13,24), and the overgrowth of dominant flora can make it hard to identify predominant species, other than fecal ones. In contrast to the luminal microbiota of the colon, small-intestinal fluid seems to harbor a different microbial population, i.e., the ratios of aerobes and anaerobes, gram-negative and gram-positive bacteria, and total bacterial counts vary between these anatomical sites (7,9,16).…”

mentioning

confidence: 99%

Comparison between Cultured Small-Intestinal and Fecal Microbiotas in Beagle Dogs

Mentula

Harmoinen

Heikkilä

et al. 2005

Appl Environ Microbiol

100

View full text Add to dashboard Cite

The microbiota of the small intestine is poorly known because of difficulties in sampling. In this study, we examined whether the organisms cultured from the jejunum and feces resemble each other. Small-intestinal fluid samples were collected from 22 beagle dogs with a permanent jejunal fistula in parallel with fecal samples. In addition, corresponding samples from seven of the dogs were collected during a 4-week period (days 4, 10, 14, and 28) to examine the stability of the microbiota. In the jejunal samples, aerobic/facultative and anaerobic bacteria were equally represented, whereas anaerobes dominated in the fecal samples. Despite lower numbers of bacteria in the jejunum (range, 10 2 to 10 6 CFU/g) than in feces (range, 10 8 to 10 11 CFU/g), some microbial groups were more prevalent in the small intestine: staphylococci, 64% versus 36%; nonfermentative gramnegative rods, 27% versus 9%; and yeasts, 27% versus 5%, respectively. In contrast, part of the fecal dominant microbiota (bile-resistant Bacteroides spp., Clostridium hiranonis-like organisms, and lactobacilli) was practically absent in the jejunum. Many species were seldom isolated simultaneously from both sample types, regardless of their overall prevalence. In conclusion, the small intestine contains a few bacterial species at a time with vastly fluctuating counts, opposite to the results obtained for the colon, where the major bacterial groups remain relatively constant over time. Qualitative and quantitative differences between the corresponding jejunal and fecal samples indicate the inability of fecal samples to represent the microbiotas present in the upper gut.

show abstract

Comparison of media for selection and enumeration of mouse fecal flora populations

Cited by 38 publications

References 6 publications

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Comparison of viable cell counts and fluorescence in situ hybridization using specific rRNA-based probes for the quantification of human fecal bacteria

Comparison between Cultured Small-Intestinal and Fecal Microbiotas in Beagle Dogs

Contact Info

Product

Resources

About