Comparison of mature retinal marker expression in Y79 and WERI-RB27 human retinoblastoma cell lines

Cassidy, Linda; Bejjani, Anthony; Choi, Meerim; Seigel, Gail M.

doi:10.5348/ocyj-2012-2-sr-1

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Here, we established an in vitro model of oxidative stress-induced retinal cell by using glutamate and aim to validate the retinal cell survivability in the presence of MSC-EPO. The Y79 cell line is a human retinoblastoma cell expressing a heterogeneous populations for both immature and mature retinal cell type-specific markers, such as Cone Rod Homeobox (CRX), Visual System Homeobox 2 (VSX2), Protein Kinase C-alpha (PKC-α), Retinoid X Receptor gamma (RXRγ), thyroid hormone receptor beta-2 (TRβ-2), Neural Retina-Specific Leucine zipper (NRL) and recoverin (Sakata and Yanagi, 2008 ; Xu et al, 2009 ; Oshikawa et al, 2011 ; Cassidy et al, 2012 ; Han and Townes-Anderson, 2012 ). Hence, it is a suitable in vitro model to represent the responses of a broader range of human retinal neuronal cells upon exposure to glutamate.…”

Section: Discussionmentioning

confidence: 99%

Human Mesenchymal Stem Cells Expressing Erythropoietin Enhance Survivability of Retinal Neurons Against Oxidative Stress: An In Vitro Study

Ding

Kumar

Khan

et al. 2018

Front. Cell. Neurosci.

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Retinal degeneration is a prominent feature in ocular disorders. In exploring possible treatments, Mesenchymal Stem Cells (MSCs) have been recognized to yield therapeutic role for retinal degenerative diseases. Studies have also displayed that erythropoietin (EPO) administration into degenerative retina models confers significant neuroprotective actions in limiting pathological cell death. In this study, we aimed to use MSCs to deliver EPO and to evaluate the ability of EPO to rescue retinal neurons from dying upon reactive oxidative stress induction. We derived human MSCs from Wharton’s jelly (hWJMSCs) of the umbilical cord and cells were transduced with lentivirus particles encoding EPO and a reporter gene of green fluorescent protein (GFP). The supernatants of both transduced and non-transduced cells were collected and used as a pre-conditioning medium for Y79 retinoblastoma cells (retinal neuron cell line) following exposure to glutamate induction. Retinal cells exposed to glutamate showed reduced mitochondrial depolarization and enhanced improvement in cell viability when incubated with pre-conditioned media of transduced cells. Our results established a proof-of-concept that MSCs could be used as a candidate for the delivery of EPO therapeutic gene in the treatment of retinal degenerations.

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Section: Discussionmentioning

confidence: 99%

Human Mesenchymal Stem Cells Expressing Erythropoietin Enhance Survivability of Retinal Neurons Against Oxidative Stress: An In Vitro Study

Ding

Kumar

Khan

et al. 2018

Front. Cell. Neurosci.

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“…A major trend in regenerative medicine requires a novel and more realistic approach via 3D tissue model, such approach is to deeply understand disease mechanism without using animals, finally to evolve treatment options (Francis & Abramson, ). In nutshell, the ARPE‐19 cells actively retain numerous RPE cell characteristics, including functional tight junctions (Dunn et al, ; Dunn et al, ), the Y79 cells may originate from early retinoblasts, arising before optic cup separation to inner and outer layers that can differentiate into either photoreceptor or glial cells, and the cell line expresses a variety of mature markers representing several retinal cell types (Cassidy, Bejjani, Choi, & Seigel, ; Chader, ; Green et al, ). The microvalve‐based bioprinting of retinal tissue model is potentially useful for drug screening, studying retinal cell interactions, and the effects of different molecules on retinal regeneration (Maenpaa, Toimela, Mannerstrom, Saransaari, & Tahti, ).…”

Section: Discussionmentioning

confidence: 99%

A bilayer photoreceptor-retinal tissue model with gradient cell density design: A study of microvalve-based bioprinting

Shi

Tan

Yeong

et al. 2018

J Tissue Eng Regen Med

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ARPE-19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, haematoxylin and eosin and immunofluorescent staining, scanning electrical microscopy and confocal microscopy, and so forth. The bioprinted ARPE-19 cells formed a high-quality cell monolayer in 14 days. Manually seeded ARPE-19 cells were poorly controlled during and after cell seeding, and they aggregated to form uneven cell layer. The Y79 cells were subsequently bioprinted on the ARPE-19 cell monolayer to form 2 distinctive patterns. The microvalve-based bioprinting is efficient and accurate to build the in vitro tissue models with the potential to provide similar pathological responses and mechanism to human diseases, to mimic the phenotypic endpoints that are comparable with clinical studies, and to provide a realistic prediction of clinical efficacy.

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Comparison of mature retinal marker expression in Y79 and WERI-RB27 human retinoblastoma cell lines

Cited by 2 publications

References 15 publications

Human Mesenchymal Stem Cells Expressing Erythropoietin Enhance Survivability of Retinal Neurons Against Oxidative Stress: An In Vitro Study

Human Mesenchymal Stem Cells Expressing Erythropoietin Enhance Survivability of Retinal Neurons Against Oxidative Stress: An In Vitro Study

A bilayer photoreceptor-retinal tissue model with gradient cell density design: A study of microvalve-based bioprinting

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