Comparison of Matrigel and Matriderm as a carrier for human amnion-derived mesenchymal stem cells in wound healing

Tuca, Alexandru; Ertl, Juliane; Hingerl, Kerstin; Pichlsberger, Melanie; Fuchs, Jakob; Wurzer, Paul; Pfeiffer, Dagmar; Bubalo, Vladimir; Parvizi, Daryousch; Kamolz, Lars‐Peter; Lang, Ingrid

doi:10.1016/j.placenta.2016.10.015

Cited by 33 publications

(31 citation statements)

References 22 publications

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“…These preclinical studies successfully demonstrated the significant therapeutic properties of hAEC as a candidate stem cell for regenerative medicine. Promising data from in vitro studies that have demonstrated the immunomodulatory properties of hAECs and have prompted a number of preclinical studies using models for lung fibrosis, liver fibrosis, wound healing, arthritis, colitis, multiple sclerosis, graft‐vs‐host disease and brain injury . Decellularized amniotic membrane has also been utilized clinically in the field of ophthalmology as a scaffold for corneal restoration.…”

Section: Therapeutic Potential Of Amniotic Epithelial Cellsmentioning

confidence: 99%

Stem cell characteristics and the therapeutic potential of amniotic epithelial cells

Miki

2018

American J Rep Immunol

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Multiple stem cell types can be isolated from the human placenta. Recent advances in stem cell biology have revealed that human amniotic epithelial cells (hAECs) are one of the perinatal stem cells which possess embryonic stem cell-like differentiation capability and adult stem cell-like immunomodulatory properties. Unlike other types of placental stem cells, hAECs are derived from pluripotent epiblasts and maintain multilineage differentiation potential throughout gestation. Similar to mesenchymal stem cells, hAECs are also able to modulate the local immune response. These, and other properties, make hAECs attractive for cellular therapy. This review article summarizes current knowledge of stem cell characteristics and immunomodulatory properties of amniotic epithelial cells and aims to advance our understanding towards the goal of novel therapy development.

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Section: Therapeutic Potential Of Amniotic Epithelial Cellsmentioning

confidence: 99%

Stem cell characteristics and the therapeutic potential of amniotic epithelial cells

Miki

2018

American J Rep Immunol

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“…Moreover, and most importantly, it is rich in stromal cells (hAMSCs) with immunomodulatory and regenerative properties [10]. Due to these features, in different preclinical inflammatory disease models, hAMSCs and their conditioned medium (CM-hAMSC) have been successfully exploited, including for lung [11][12][13][14] and liver fibrosis [15], wound healing [16][17][18], collagen-induced arthritis [19,20], multiple sclerosis [19], inflammatory bowel disease, colitis [21], sepsis [19], traumatic brain injury [22], and Huntington's disease [23]. Accordingly, in vitro, we and others have demonstrated that hAMSCs and CM-hAMSC suppress the proliferation, inflammatory cytokine production, and functions of T lymphocytes [24,25], monocytes [18], dendritic cells [26], macrophages [18], and natural killer cells [27], while inducing a phenotype and functional switch of monocytes toward macrophages with anti-inflammatory pro-regenerative M2-like features [18,25], supporting the expansions of regulatory T cells [24,25].…”

Section: Introductionmentioning

confidence: 99%

miRNA Reference Genes in Extracellular Vesicles Released from Amniotic Membrane-Derived Mesenchymal Stromal Cells

et al. 2020

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Human amniotic membrane and amniotic membrane-derived mesenchymal stromal cells (hAMSCs) have produced promising results in regenerative medicine, especially for the treatment of inflammatory-based diseases and for different injuries including those in the orthopedic field such as tendon disorders. hAMSCs have been proposed to exert their anti-inflammatory and healing potential via secreted factors, both free and conveyed within extracellular vesicles (EVs). In particular, EV miRNAs are considered privileged players due to their impact on target cells and tissues, and their future use as therapeutic molecules is being intensely investigated. In this view, EV-miRNA quantification in either research or future clinical products has emerged as a crucial paradigm, although, to date, largely unsolved due to lack of reliable reference genes (RGs). In this study, a panel of thirteen putative miRNA RGs (let-7a-5p, miR-16-5p, miR-22-5p, miR-23a-3p, miR-26a-5p, miR-29a-5p, miR-101-3p, miR-103a-3p, miR-221-3p, miR-423-5p, miR-425-5p, miR-660-5p and U6 snRNA) that were identified in different EV types was assessed in hAMSC-EVs. A validated experimental pipeline was followed, sifting the output of four largely accepted algorithms for RG prediction (geNorm, NormFinder, BestKeeper and ΔCt method). Out of nine RGs constitutively expressed across all EV isolates, miR-101-3p and miR-22-5p resulted in the most stable RGs, whereas miR-423-5p and U6 snRNA performed poorly. miR-22-5p was also previously reported to be a reliable RG in adipose-derived MSC-EVs, suggesting its suitability across samples isolated from different MSC types. Further, to shed light on the impact of incorrect RG choice, the level of five tendon-related miRNAs (miR-29a-3p, miR-135a-5p, miR-146a-5p, miR-337-3p, let-7d-5p) was compared among hAMSC-EVs isolates. The use of miR-423-5p and U6 snRNA did not allow a correct quantification of miRNA incorporation in EVs, leading to less accurate fingerprinting and, if used for potency prediction, misleading indication of the most appropriate clinical batch. These results emphasize the crucial importance of RG choice for EV-miRNAs in hAMSCs studies and contribute to the identification of reliable RGs such as miR-101-3p and miR-22-5p to be validated in other MSC-EVs related fields.

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“…Dermal equivalents are used as off‐the‐shelf products in clinics such as MatriDerm®, Integra™, and DermaGraft®. These grafts combine autologous dermal fibroblasts and matrices based on various artificial, natural and synthetic materials, respectively . Combinations of epidermal and dermal grafts include PermaDerm™ and DenovoSkin, which are based on natural scaffold materials, fibroblasts and keratinocytes covering superficial wounds .…”

Section: Introductionmentioning

confidence: 99%

“…These grafts combine autologous dermal fibroblasts and matrices based on various artificial, natural and synthetic materials, respectively. [8][9][10][11][12] Combinations of epidermal and dermal grafts include PermaDerm™ and DenovoSkin, which are based on natural scaffold materials, fibroblasts and keratinocytes covering superficial wounds. 13 Issues that remain to be addressed include the risk of infection, poor adhesion and the temporary nature of the application.…”

Section: Introductionmentioning

confidence: 99%

Macrophages significantly enhance wound healing in a vascularized skin model

Kreimendahl

Marquardt

Apel

et al. 2019

J Biomedical Materials Res

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Tissue‐engineered dermo‐epidermal skin grafts could be applied for the treatment of large skin wounds or used as an in vitro wound‐healing model. However, there is currently no skin replacement model that includes both, endothelial cells to simulate vascularization, and macrophages to regulate wound healing and tissue regeneration. Here, we describe for the first time a tissue‐engineered, fully vascularized dermo‐epidermal skin graft based on a fibrin hydrogel scaffold, using exclusively human primary cells. We show that endothelial cells and human dermal fibroblasts form capillary‐like structures within the dermis whereas keratinocytes form the epithelial cell layer. Macrophages played a key role in controlling the number of epithelial cells and their morphology after skin injury induced with a CO2 laser. The activation of selected cell types was confirmed by mRNA analysis. Our data underline the important role of macrophages in vascularized skin models for application as in vitro wound healing models or for skin replacement therapy. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1340–1350, 2019.

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Comparison of Matrigel and Matriderm as a carrier for human amnion-derived mesenchymal stem cells in wound healing

Cited by 33 publications

References 22 publications

Stem cell characteristics and the therapeutic potential of amniotic epithelial cells

Stem cell characteristics and the therapeutic potential of amniotic epithelial cells

miRNA Reference Genes in Extracellular Vesicles Released from Amniotic Membrane-Derived Mesenchymal Stromal Cells

Macrophages significantly enhance wound healing in a vascularized skin model

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