Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

Abstract

Objective: The differences between type 1 and type 2 diabetes mellitus (T1DM and T2DM) in terms of their adverse effects on male reproductive parameters have never been elucidated. This study aimed to distinguish between the effects of the DM types in mice treated with multiple low doses of streptozotocin (STZ) to mimic human T1DM and coadministered a high-fat diet (HFD) to mimic human T2DM.Methods: The T1DM mice were intraperitoneally injected with STZ (40 mg/kg body weight) for 5 days. The T2DM mice received an HFD for 14 days prior to STZ injection (85 mg/kg body weight), followed by continuous feeding of an HFD. Male reproductive parameters were evaluated.Results: The reproductive organs of the DM mice weighed significantly less than those of controls, and the seminal vesicles plus prostates of the T1DM mice weighed less than those of the T2DM mice. Increased sperm abnormalities and incomplete DNA packaging were observed in the DM groups. Sperm concentration and the proportion of normal sperm were significantly lower in the T1DM group. The seminiferous histopathology of DM mice was classified into seven types. The penises of the DM mice were smaller than those of the controls; however, tunica albuginea thickness and the amount of penile collagen fibers were increased in these mice. Round germ cells were abundant in the epididymal lumens of the mice with DM.Conclusion: T1DM adversely affected reproductive parameters to a greater extent than T2DM.

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

“…It is possible that DSF can stimulate insulin production declining of metabolic rates. Indeed, several investigations have also demonstrated the significant decrease of male reproductive organ weights in T2DM rats (Sampannang et al, 2019(Sampannang et al, , 2020Yannasithinon & Iamsaard, 2019). Similar to many studies, we also demonstrated detrimental effect of DM on sperm parameters (La Vignera et al, 2012;Liu et al, 2013;Maremanda et al, 2020;Sampannang et al, 2020).…”

“…It is possible that DSF can stimulate insulin production declining of metabolic rates. Indeed, several investigations have also demonstrated the significant decrease of male reproductive organ weights in T2DM rats (Sampannang et al, 2019(Sampannang et al, , 2020Yannasithinon & Iamsaard, 2019). Herein, DSF could improve reproductive organ weights by increasing of diameter and height of seminiferous tubules, tunica albuginea thickness and epididymal sperm density.…”

“…Currently, a number of people with DM are increasing every year and it is known that DM especially type 2 (T2DM) can impair many systems including the reproductive system of human and rodent animal models. Indeed, T2DM can cause male infertility by affecting the spermatogenesis and testosterone synthesis (Sampannang et al, 2017(Sampannang et al, , 2018(Sampannang et al, , 2019(Sampannang et al, , 2020Yannasithinon & Iamsaard, 2019). However, the mechanism underlying male infertility caused by T2DM is still not completely understood at the physiological and molecular levels.…”

“…In addition, the changes of testicular androgen receptor (AR) and heat-shock protein 70 (Hsp70) may be involved in germ cell differentiation and associated with the apoptotic process (Abd EL-Meseeh et al, 2016;Koeva et al, 2005;Rockett et al, 2001;Zhu et al, 2000). Especially, expressions of the steroidogenic acute regulatory (StAR) protein and tyrosine-phosphorylated (TyrPho) proteins in testis have been demonstrated to play the important roles in spermatogenesis and androgen production (Arun et al, 2016;Sampannang et al, 2017Sampannang et al, , 2018Sampannang et al, , 2019Sampannang et al, , 2020. It is possible that the impairment in expressions of those testicular proteins may be involved in sub-fertility in male T2DM.…”

Tribulus terrestris Efficacy and Safety Concerns in Diabetes and Erectile Dysfunction, Assessed in an Experimental Model

“…Studies with animal models of STZ-induced diabetes have shown that both type 1 and type 2 DZ alter male fertility by modifying sperm specific parameters (sperm motility, sperm DNA integrity and sperm morphology) [ 18 , 19 ]. These changes may occur as a result of impaired spermatogenesis, but also due to inhibition of intracellular glucose transport as a result of insulin deficiency.…”