Comparison of Major Phenolic Constituents and in Vitro Antioxidant Activity of Diverse Kudingcha Genotypes from Ilex kudingcha, Ilex cornuta, and Ligustrum robustum

Abstract

A total of seven Kudingcha genotypes from three plant species (Ilex kudingcha, Ilex cornuta, and Ligustrum robustum) with different geographic origins in China were investigated for their major phenolic compounds, individual and total phenolics contents, and in vitro antioxidant properties (ABTS, DPPH, FRAP, and OH assays). LC-PDA-APCI-MS analysis showed that Kudingcha genotypes from Ilex and Ligustrum had entirely different phenolic profiles. Major phenolics in Kudingcha genotypes from two Ilex species were mono- and dicaffeoylquinic acids, whereas those in a Kudingcha genotype from Ligustrum were phenylethanoid and monoterpenoid glycosides. All Kudingcha genotypes of Ilex exhibited significantly stronger antioxidant capacities than that of Ligustrum. Within six Ilex genotypes, great variation existed in their composition of individual phenolic compounds and their antioxidant properties. The comparative data and LC fingerprints obtained in this study may provide useful information for screening and breeding of better Kudingcha genotypes and also for their authentication and quality control.

Evaluation of the antioxidant activity of five endemic Ligustrum species leaves from Taiwan flora in vitro

“…The IC 50 values for L. morrisonense, L. liukiuense, L. pricei, L. sinense, and L. lucidum in the DPPH assay were 0.48, 0.94, 1.38, 1.47, and 2.06 mg/ml, respectively. The results obtained from the DPPH assay is similar to recent reports of other Ligustrum species, including L. lucidum and L. robustum (Chen, Wong, Leung, He, & Huang, 2002;Katsube et al, 2004;Nagy et al, 2006;She, Wang, Zeng, Yang, & Zhang, 2008;Zhu et al, 2009). For the TEAC assay, the IC 50 values for L. morrisonense, L. liukiuense, L. pricei, L. sinense, and L. lucidum were 0.21, 0.24, 0.37, 0.52, and 1.40 mg/ml, respectively.…”

“…The FRAP assay described by Zhu et al (2009) was used with some modifications. Briefly, 25 l of sample solution or Trolox standards was mixed with 25 l of freshly prepared FRAP reagent, which consisted of 10 mM TPTZ solution in 40 mM HCl, 20 mM FeCl 3 , and 50 mM acetate buffer (pH 3.6).…”

“…In recent years, numerous plants belonging to the same genera have been reported to characterise antioxidant and radicalscavenging activities (Tepe, 2008;Tepe & Sokmen, 2007;Zhu et al, 2009). Some species from the genus Ligustrum (Oleaceae), including Ligustrum lucidum, Ligustrum robustum, and Ligustrum purpurascens, have been traditionally used as healthy tea or local medicine in European, Chinese, and Japanese communities for centuries.…”

Ilex latifoliaPrevents AmyloidβProtein (25–35)-Induced Memory Impairment by Inhibiting Apoptosis and Tau Phosphorylation in Mice

“…12,[16][17][18] Since oxidative stress plays a key role in Ab-induced neurotoxicity, 4,42 antioxidants have been studied to exploit their neuroprotective effects in AD models. CQAs have been reported to protect neurons against Ab-promoted neuronal cell damage.…”

“…10 Thus, the antioxidant properties and phenolic compounds of I. latifolia have been evaluated. 11,12 Due to the antioxidant activity of I. latifolia, neuroprotective effects of this plant have also been examined. 13 In a previous study, we demonstrated that the antioxidative and anti-inflammatory effects of I. latifolia provided protection against apoptotic neuronal death associated with transient focal ischemia-induced brain damage.…”