Comparison of macro-morphological and physiological methods for<i>Monilinia</i>species identification in Paraná State, Brazil

Lichtemberg, Paulo S. F.; Silva, Francinéia A.; Zeviani, Walmes M.; Mio, Louise Larissa May De

doi:10.1080/07060661.2013.864710

Cited by 8 publications

(15 citation statements)

References 19 publications

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“…Lichtemberg et al . () also reported some Brazilian M. fructicola isolates with limited sporulation, as was found in the current study.…”

Section: Discussionsupporting

confidence: 87%

“…However, these techniques are not always sufficient for accurate isolate characterization at species level. As mentioned by other authors, morphological diversity and the presence of atypical colonies hinder accurate species identification (Lichtemberg et al ., ). In the current study, some M. fructicola isolates displayed limited and scattered sporulation and lobed colony margins.…”

Section: Discussionmentioning

confidence: 97%

“…However, there is no report about the genetic variability of the Brazilian population. Molecular tools, such as species‐specific PCR, associated with morphological characterizations, have been used to identify Monilinia isolates (van Leeuwen & van Kesteren, ; Lane, ; Côté et al ., ; May De Mio et al ., ; Lichtemberg et al ., ), but these techniques are unsuitable for genetic diversity and variability assessments.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

et al. 2016

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Fungi of the Monilinia genus occur worldwide and affect a wide range of economically important stone fruits. Several Monilinia species are responsible for brown rot. Although this disease is common in Brazil, Monilinia sp. genetic variability in Brazilian orchards has generally been poorly characterized. The present study represents the first report on the genetic diversity of Monilinia sp. from Brazilian orchards. The genetic structure of the Brazilian population was also compared to isolates from other countries, together with some morphological characteristics and aggressiveness. Sixty‐one isolates belonging to the Monilinia genus were obtained from different orchards in Brazilian states. Ten Monilinia fructicola isolates from the United States and one isolate from a fruit imported into Brazil were also evaluated. Phylogenetic analysis of the ITS1‐5.8S‐ITS2 region (internal transcribed spacer) clustered most Brazilian and American isolates with M. fructicola authentic strains from Q‐Bank. Two isolates (one from an imported fruit) clustered as Monilinia laxa. The results revealed M. fructicola as the prevalent species associated with brown rot in Brazilian orchards. To evaluate the intraspecific diversity of M. fructicola and M. laxa, multigene sequence analysis was performed using ITS1‐5.8S‐ITS2 and TEF1 (elongation factor 1). Whilst TEF1 is the most phylogenetically informative gene for intraspecific studies of M. fructicola, RPB2 (RNA polymerase II gene) displayed low variation in intraspecific analysis, but was an informative locus for assigning isolates to M. fructicola or M. laxa species. The amova suggests that Brazilian isolates from the States of the main producing regions belong to a single genetic population, which is genetically distinct from the US (Californian) population of M. fructicola.

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“…Lichtemberg et al . () also reported some Brazilian M. fructicola isolates with limited sporulation, as was found in the current study.…”

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

et al. 2016

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“…For Teixeira et al (2004), the morphophysiological variability may be influenced by some factor intrinsic to the pathogen, to the host genotype, or even to the interaction between them. According to the data obtained in the present study, the morphological characterization presents many variations in the topography of colonies of isolates between the culture media used, a result also verified by Lichtemberg et al (2014) when evaluating Monilinia spp. isolates in acidified PDA culture medium.…”

Section: Isolatesmentioning

confidence: 70%

“…In the present study, in the Pagnocca medium, the predominant coloration of the isolates was white whereas in the peach juice medium there was a small variation, from white to rosy, according to the Munsell Color table (1954) (Figure 4). Lichtemberg et al (2014) also found that M. fructicola isolates showed differences in coloration, varying in shades of gray, yellow/cream and white. These differences, according to Pereira et al (2006), indicate that most of the fungi differ in their morphological characteristics according to the modifications in the development substrate.…”

Section: Isolatesmentioning

confidence: 95%

Identification and variability of Monilinia spp. isolates from peach

Casarin

Casa-Coila

Rossetto

et al. 2017

Agraria

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The brown rot caused by the fungus Monilinia spp. is the main disease of peach (Prunus persica) that occurs from flowering to post-harvest fruit. The objective of this study was to identify the species that causes brown rot in Rio Grande do Sul state using molecular method based on specific primers and study the morphological variability of Monilinia isolates using classic characterization methods. The isolates were collected in the main producing regions of peaches and determination of species was performed by using specific primers to the genus Monilinia. The culture media BDA  , peach juice and Pagnocca were used for the morphological characterization of isolates. Molecular study showed that all isolates from Rio Grande do Sul are the species M. fructicola. In the morphological characterization, such as mycelial diameter, color, margin, shape and elevation of the colonies and dry mass of mycelium, there are variability in the analyzed characteristics of the isolates in the three-culture media used. In the comparing the culture media, the higher dry matter values and diameter of colony isolates were obtained in Pagnocca and peach juice means. Key words: phenotypic characterization; brown rot; Prunus persica Identificação e variabilidade de isolados de Monilinia spp. de pessegueiro RESUMOA podridão parda causada pelo fungo Monilinia spp. é a principal doença que ocorre desde a floração até a pós-colheita de frutos de pessegueiro (Prunus persica) no Brasil. O objetivo deste trabalho foi identificar a espécie causadora da podridão parda no Rio Grande do Sul usando método molecular baseado em primers específicos e estudar a variabilidade morfológica de isolados de Monilinia através dos métodos clássicos de caracterização. Os isolados foram coletados nas principais regiões produtoras de pêssegos e a determinação da espécie foi realizada através da utilização de primers específicos para o gênero Monilinia. Os meios de cultura BDA  , Suco de pêssego e Pagnocca foram utilizados para a caracterização morfológica dos isolados. Pelo estudo molecular verificou-se que todos os isolados do Rio Grande do Sul são da espécie M. fructicola. Na caracterização morfológica, como diâmetro micelial, coloração, margem, forma e elevação das colônias e massa seca de micélio, existem variabilidade nas características analisadas dos isolados nos três meios de cultura utilizados. Na comparação entre os meios de cultura, os maiores valores de massa seca e diâmetro de colônia dos isolados foram obtidos nos meios Pagnocca e suco de pêssego.Palavras-chave: caracterização fenotípica; podridão parda; Prunus persica

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Comparative in vivo and in vitro study on Monilia fructicola causing brown rot of stone fruit in Brazil and California

Lichtemberg

Zeviani

Michailides

et al. 2016

Trop. plant pathol.

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Stone fruit production plays an important economical role in southern Brazil as well as in California, United States. In both countries, brown rot caused by Monilinia fructicola is the most important stone fruit disease, responsible for significant pre-and post-harvest fruit losses. The United States is among the main importers of stone fruit to Brazil. Imported fruit carrying the pathogen may introduce isolates with greater ability to infect stone fruit orchards in Brazil. In this study, 26 M. fructicola isolates from Brazil and California, recovered from commercial stone fruit orchards, were randomly selected and compared based on in vitro (optimum growth temperature, mycelial growth rate, germination, spore production and germ tube rate) and in vivo (sporulation, area under disease progress curve, incubation and latent period) studies on brown rot infection components. The isolates behaved similarly for most of the studied variables, except for germ tube length and sporulation on fruit, which were higher in isolates from California and Brazil, respectively. The negative correlation between these variables may be disadvantage for isolates from California to cause infections in the main peach cultivar grown in South Brazil. Significant differences in incubation and latent period and the area under disease progress curve were found among isolates from a same country. Such differences may be due to intraspecific diversity associated with the occurrence of the perfect stage in California, and by the broad sampling area from where isolates from Brazil were obtained. It is still difficult to determine the effect of isolates arising from imported California fruits in Brazilian stone fruit orchards. Further genetic variability studies and comparative epidemiology in the field may help to understant the real threat that foreign isolates represent.

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Comparison of macro-morphological and physiological methods forMoniliniaspecies identification in Paraná State, Brazil

Cited by 8 publications

References 19 publications

Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

Identification and variability of Monilinia spp. isolates from peach

Comparative in vivo and in vitro study on Monilia fructicola causing brown rot of stone fruit in Brazil and California

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