Comparison of Lytic and Persistent Measles Virus Matrix Proteins by Competition Radioimmunoassay

Abstract

SUMMARYMeasles virus matrix (M) proteins were compared by competitive monoclonal antibody-binding studies.

Effect of Measles Virus Antibodies on a Measles SSPE Virus Persistently Infected C6 Rat Glioma Cell Line

“…Only monoclonal antibodies directed against the haemagglutinin of measles virus were effective in modulation. A number of different monoclonal antibodies directed against the H protein have been obtained in our laboratory (ter Meulen et al, 1981 ;Carter et al, 1983). Antibodies were selected which were all known to bind to the membranes of cells persistently infected with SSPE virus Lec or to immunoprecipitate the H protein of this virus (ter Meulen et al, 1981 ;Carter et al, 1983).…”

“…A number of different monoclonal antibodies directed against the H protein have been obtained in our laboratory (ter Meulen et al, 1981 ;Carter et al, 1983). Antibodies were selected which were all known to bind to the membranes of cells persistently infected with SSPE virus Lec or to immunoprecipitate the H protein of this virus (ter Meulen et al, 1981 ;Carter et al, 1983). All of these antibodies were able to induce capping reactions on the C6/SSPE cell membrane and to strip membrane antigen from the surface of the cell; however, they differed in their effects on intracellular virus antigen expression (Table 2).…”

Adaptation of Coronavirus JHM to Persistent Infection of Murine Sac(-) Cells

“…0022-1317/84/0000-6005 $02.00 0 1984 SGM 1971 ; Thacore & Youngner, 1969) or structure Yoshikura & Tejima, 1981 ;Carter et al, 1983 ;Makino et al, 1983) during persistence. Such alterations are therefore of direct relevance in chronic coronavirus infection of the animal.…”

Measles Virus Persistent Infection: Modification of the Virus Nucleocapsid Protein

“…However, these observations offer only a partial explanation for measles virus persistence, since in several persistently infected (p.i.) systems, synthesis of all virus proteins, including M protein, was detectable (Rozenblatt et al, 1979 a;Rentier et al, 1981 ;Carter et al, 1983). In these cases, nonbudding measles nucleocapsids were found misaligned within the cell (Knight et al, 1973;Dubois-Dalcq et al, 1976) and possessed an altered conformation (Rozenblatt et al, 1979b).…”

“…Measles virus, a paramyxovirus associated with the rare neurological disease subacute sclerosing panencephalitis (SSPE), has been extensively studied because of its ability to give rise to persistent infections. Most of the studies have highlighted the role of the matrix (M) protein in defective viral maturation, namely (i) selective lack of synthesis of M protein (Hall & Choppin, 1979), (ii) increased degradation rate or a defect in accumulation of M protein (Roux & Waldvogel, 1982;Sheppard et al, 1985;Young et al, 1985) or (iii) structural changes of M protein presumably associated with viral mutations (Wechsler et al, 1979b;Wild et al, 1979;Carter et al, 1983). However, these observations offer only a partial explanation for measles virus persistence, since in several persistently infected (p.i.)…”