Comparison of lysis-centrifugation with lysis-filtration and a conventional unvented bottle for blood cultures

Abstract

Evaluation of a commercially available lysis-centrifugation blood culture system (Isolator, DuPont Co., Wilmington, Del.) and a lysis-filtration blood culture system for 3,111 cultures showed that both methods had comparable recoveries (73 and 68%, respectively) of significant aerobic and facultatively anaerobic isolates. The unvented conventional blood culture bottle had a recovery rate of 59%. Although the lysis-centrifugation and lysis-filtration systems had comparable recoveries of pathogens, the lysis-centrifugation system had the advantage of having colonies immediately available for further testing. The contamination rate with the lysiscentrifugation system was 3%, compared with 6% with the lysis-filtration system and 0.4% with brain heart infusion.

Detection and quantitation by lysis-filtration of bacteremia after different oral surgical procedures

“…The observed incidences as well as magnitudes of bacteremia were higher than those described previously, confirming the high sensitivity of the combination of lysis-filtration and brain heart infusion agar (6). Asymptomatic bacteremia (7,16) and a high frequency of contamination have been reported in connection with blood culture systems based on lysis of blood (5,6), and therefore, preoperative blood samples must also be obtained in order to estimate the true postoperative bacteremia. In the present study, we decreased the frequency of contamination from 7%, as reported previously (6), to 0% in 300 blood samples by changing the laboratory procedures from daily microscopic examination to one single examination after 10 days.…”

Controlled comparison of the BACTEC high-blood-volume fungal medium, BACTEC Plus 26 aerobic blood culture bottle, and 10-milliliter isolator blood culture system for detection of fungemia and bacteremia

“…IS previously has been shown to be inferior to radiometric BACTEC bottles for recovery of all streptococci combined and for viridans group streptococci (5). Although the IS tube is an acceptable method for detecting bacteria, this system has been reported to have a higher contamination rate relative to that of BACTEC bottles and to be inferior to other systems for recovery of certain bacteria (particularly streptococci, pseudomonads, and anaerobic bacteria) (5,7,14,15,17).…”

Evaluation and comparison of different blood culture techniques for bacteriological isolation of Salmonella typhi and Brucella abortus

“…The findings for the WBL-F and FH-CF blood culture techniques differ from those of other existing reports. Previous works on the subject (1,10,13) showed that the centrifugation filtration and lysis filtration techniques (6,34,35) were able to detect bacteria quicker than conventional blood culture techniques. Nevertheless, these techniques are cumbersome not only because of the excessive handling of the sample but also because of the potential clogging of the filtration system with blood debris (28,29).…”

“…Once the product was filtered, the side of the filter that had been in contact with the bacteria was placed on trypticase soy agar to plate the bacteria. As soon as bacterial growth was detected, identification tests were carried out (6,9,23,(28)(29)(30)35). Eighteen blood cultures for B. abortus and 18 for S. typhi were carried out.…”