Comparison of&lt;i&gt; in vitro &lt;/i&gt;digestibility using slaughtered and fistulated cattle as sources of inoculum

Beyihayo, GA; Omaria, Rose; Namazzi, C; Atuhaire, Andrew M.

doi:10.4314/ujas.v16i1.7

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Quatro jarros foram utilizados e uma rodada de análises foi realizada. O fluido ruminal de bovino foi obtido no frigorifico Fribarreiras (Barreiras, Bahia, Brasil) no momento do abate, o qual é adequado para utilização nesta metodologia (BEYIHAYO et al, 2015). Foram coletados 5 litros de fluído, o qual foi depositado em uma garrafa térmica pré-aquecida, mantendo-se a temperatura de 39°C.…”

Section: Methodsunclassified

Valor nutritivo de genótipos comerciais de sorgo forrageiro cultivados no oeste da Bahia

Quadros¹,

Chagas²,

Santos³

et al. 2021

Rev Agr Acad

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The objective was to evaluate the nutritive value of plants and silage of seven genotypes of sorghum (Podium, Formoso, BRS-610, IPA-1011, IPA-467, SF-15 and SS-318). A RBD with three replications was used to evaluate nutritive value of the plants cultivated in the municipalities of Barreiras and Muquem do Sao Francisco/BA, Brazil, while a CRD with three replications was used to evaluate the nutritive value of the silages from the plants cultivated in Barreiras. Chemical composition of forage and silage varied according to the genotype. However, the most remarkable effects were on CP, NDF, ADF and IVDMD. The best sorghum genotypes in terms of nutritive value were Podium, IPA-1011 e BRS-610.

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Section: Methodsunclassified

Valor nutritivo de genótipos comerciais de sorgo forrageiro cultivados no oeste da Bahia

Quadros¹,

Chagas²,

Santos³

et al. 2021

Rev Agr Acad

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“…Rumen fluid was collected from slaughterhouse animals (rams) following the protocol of Fortina et al (2022) [49], with small modifications. It was decided to use rumen fluid collected from slaughterhouse animals, first, because a significant difference in in vitro digestibility has not been found when the fluid was obtained from slaughtered or fistulated ruminants [50], and, second, because this was a more ethically acceptable approach [51]. It was reported that it is possible to store the rumen fluid without significant quality changes by putting it in thermic bottles wrapped in a thermic bag, for a period of up to 300 min after collection [49].…”

Section: Determination Of Released Gas Emissions In Vitro Analysismentioning

confidence: 99%

Granulated Animal Feed and Fuel Based on Sea Buckthorn Agro-Waste Biomass for Sustainable Berry Production

et al. 2023

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The industrial harvesting of sea buckthorn (SBT) berries with twigs and subsequent pruning creates a large volume of lignocellulosic agro-waste. This study aimed to valorize this agro-waste as a raw material for animal feed and fuel granules, for developing a sustainable cascading SBT production scheme. Five SBT cultivars’ biomasses were characterized by analytical pyrolysis, mass spectrometry, and GC analysis. Condensed tannins, which are undesirable components for animal feed, were separated by extraction. The residue was analyzed for total protein, vitamins (A, C, and E), ash, crude fat, wood fiber, and macroelements (P, K, Ca, and Na), and showed great potential. The heavy metal (Cd, Hg, and Pb) content did not exceed the permitted EU maximum. Granulation regimes were elaborated using a flat-die pelletizer, KAHL 14-175. The digestibility and the amount of produced gas emissions were determined using in vitro systems that recreate the digestion of small ruminants. The investigation proved that SBT leaves and stems are a unique underutilized source of animal feed, used alone or in combination with others. Twigs, due to their thorns, were granulated and valorized according to standards for application as fuel. The scheme offered in this study enables SBT agro-waste utilization and sustainable SBT berry production.

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“…A more ethically acceptable approach that reduces stress and alleviates the suffering of animals by avoiding an invasive procedure is the collection of RF at slaughtering [40]. Alba et al [41] verified, through the use of an AD II incubator, that the rumen inoculum obtained from slaughtered cattle can be used to replace the use of cannulated animals and that this approach is a viable alternative to digestibility analysis.…”

Section: Rumen Fluidmentioning

confidence: 99%

In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

Tassone

Fortina

Peiretti

2020

Animals

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This review summarises the use of the Ankom DaisyII incubator (ADII; Ankom Technology Corporation Fairport, NY, USA), as presented in studies on digestibility, and its extension to other species apart from ruminants, from its introduction until today. This technique has been modified and adapted to allow for different types of investigations to be conducted. Researchers have studied and tested different procedures, and the main sources of variation have been found to be: the inoculum source, sample size, sample preparation, and bag type. In vitro digestibility methods, applied to the ADII incubator, have been reviewed, the precision and accuracy of the method using the ADII incubator have been dealt with, and comparisons with other methods have been made. Moreover, some hypotheses on the possible evolutions of this technology in non-ruminants, including pets, have been described. To date, there are no standardised protocols for the collection, storage, and transportation of rumen fluid or faeces. There is also still a need to standardise the procedures for washing the bags after digestion. Moreover, some performance metrics of the instrument (such as the reliability of the rotation mechanism of the jars) still require improvement.

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Comparison of<i> in vitro </i>digestibility using slaughtered and fistulated cattle as sources of inoculum

Cited by 4 publications

References 13 publications

Valor nutritivo de genótipos comerciais de sorgo forrageiro cultivados no oeste da Bahia

Valor nutritivo de genótipos comerciais de sorgo forrageiro cultivados no oeste da Bahia

Granulated Animal Feed and Fuel Based on Sea Buckthorn Agro-Waste Biomass for Sustainable Berry Production

In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

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