Comparison of &lt;i&gt;Festuca&lt;/i&gt; Leaf Streak Virus Antigens with Those of Three Other Rhabdoviruses Infecting the Gramineae

Lundsgaard, T.

doi:10.1159/000149533

Cited by 11 publications

(8 citation statements)

References 3 publications

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“…Formation of more than one virus-specific precipitin band in agar-geidiffusion is not uncommon in rhabdoviruses. For instance, northern cereal mosaic virus (NCMV) (LUNDSGAARD et al 1984), CCMV (LOCKHART 1986, LUNDSGAARD'-1984 and CCSV (LOCKHART er^/. 1985) may produce two precipitin lines with homologous antiserum.…”

Section: Discussionmentioning

confidence: 99%

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus¹)

Izadpanah¹

1989

Journal of Phytopathology

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Leaves of maize infected with the Iranian maize mosaic rhabdovirus (IMMRV) were homogenized in 0.1 M citrate‐0.04 M Na2SO3 buffer, pH 5.4, containing 10 % sucrose and the extract was subjected to low speed and high speed centrifugation followed by resuspension in 0.05 M potassium phosphate buffer, pH 7.2, containing 10 % sucrose. Partially purified preparation was obtained by density‐gradient centrifugation, removal of the virus zones and their concentration by high speed centrifugation. Two virus specific bands were observed in density‐gradient columns. An antiserum with a titer of 128 was prepared by injecting partially purified virus into rabbits. In agar‐gel‐diffusion tests, the antiserum produced one or two precipitin lines against diseased maize extract but none against healthy maize extract. IMMRV was not related to barley yellow striate mosaic (BYSMV), cereal chlorotic mottle (CCMV), Cynodon chlorotic streak (CCSV), Festuca leaf streak, and maize mosaic (MMV) viruses as well as to two unidentified rhabdoviruses occurring in wheat and Bermuda grass in the vicinity of Shiraz, when these viruses were tested against IMMRV antiserum in agar‐gel‐diffusion and enzyme‐linked immunosorbent assay. Likewise, IMMRV did not react with antisera to BYSMV, CCMV, CCSV and MMV in agar‐gel‐diffusion tests. IMMRV appears to be different from most reported rhabdoviruses of cereals.

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Section: Discussionmentioning

confidence: 99%

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus¹)

Izadpanah¹

1989

Journal of Phytopathology

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“…Wheat chlorotic streak virus (WCSV) Rhabdoviruses of plants have been given many different names but it is not clear how many distinct viruses are represented, be cause few comparisons have been reported between different isolates studied in one la boratory, and few antisera exist. The method of Lundsgaard [1] and Lundsgaard et al [2] permits serological comparison of the nucle ocapsids of rhabdovirus particles extracted from France (6,7], maize sterile stunt virus (MSSV) from Australia [8], and Shiraz maize rhabdovirus (SMRV) from Iran [9] were supplied as air-dried or lyophilized infected leaves of wheat and maize by P.A. Signoret [12].…”

Section: Virusesmentioning

confidence: 99%

“…The method of Lundsgaard [ 1,2] was followed, all operations being carried out at room temperature. Leaf material was homogenized in a small amount of 0.1 M phosphate buffer, pH 7.0 (PB) containing 1% (v/v) Nonidet P-40.…”

Section: Titration O F Antiseramentioning

confidence: 99%

Serological Relationships between the Nucleocapsids of Some Planthopper-Borne Rhabdoviruses of Cereals

Milne¹,

Masenga²,

Conti³

1986

Intervirology

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Leaves infected with barley yellow striate mosaic virus (BYSMV) from Italy, wheat chlorotic streak virus (WCSV) from France, northern cereal mosaic virus (NCMV) from Japan, maize sterile stunt virus (MSSV) from Australia or Shiraz maize rhabdovirus (SMRV) from Iran were homogenized in buffered 1% Nonidet P-40, releasing intact nucleocapsids. These (except SMRV) were trapped on electron microscope grids using appropriate antisera and tested by decoration with serial dilutions of antisera to BYSMV, NCMV, MSSV, Moroccan wheat rhabdovirus (MWRV), wheat rosette stunt virus (WRSV) from China, and maize mosaic virus (MMV) from Venezuela. The results suggest that (i) BYSMV and NCMV, though related, are distinct viruses; (ii) MWRV and WCSV are strains of BYSMV; (iii) MSSV is intermediate between BYSMV and NCMV but may be considered a strain of BYSMV; (iv) WRSV is a strain of NCMV; and (v) MMV and SMRV are unrelated to each other and to the other viruses tested. SMRV was morphologically quite different from BYSMV, NCMV, WCSV and MSSV, all of which had the same morphology.

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“…Two other viruses in the genus Cytorhabdovirus are transmitted in the persistent manner by delphacid planthoppers, namely Barley yellow striate mosaic virus (BYSMV) (Conti, 1980) and Northern cereal mosaic virus (NCMV) (Yamada and Shikata, 1969). Serological relationship has been shown between BYSMV and NCMV (Milne et al, 1986), but not between FLSV and BYSMV or NCMV (Lundsgaard, 1984). Aggregates of enveloped BYSMV virions accumulate in membranous sacs close to viroplasms in the cytoplasm of salivary glands from infected Laodelphax striatellus (Fallén).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of viruliferous Javesella pellucida transmitting Festuca leaf streak virus (genus Cytorhabdovirus)

Lundsgaard

2018

Preprint

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The ultrastructure of cells in the head and thorax from viruliferous Javesella pellucida transmitting Festuca leaf streak virus was studied. Aggregates of nonenveloped nucleocapsid particles were observed at the periphery of viroplasms located in cytoplasm of salivary gland cells, fat cells, and nerve cell bodies. Aggregates of nucleocapsid particles, not associated with viroplasms, were seen within a distance of about 1 µm from the basal lamina of salivary glands. Enveloped virions, singly or aggregated, were observed in nerve cell axons and/or dendrites.

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Comparison of <i>Festuca</i> Leaf Streak Virus Antigens with Those of Three Other Rhabdoviruses Infecting the Gramineae

Cited by 11 publications

References 3 publications

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus¹)

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus¹)

Serological Relationships between the Nucleocapsids of Some Planthopper-Borne Rhabdoviruses of Cereals

Ultrastructure of viruliferous Javesella pellucida transmitting Festuca leaf streak virus (genus Cytorhabdovirus)

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Comparison of <i>Festuca</i> Leaf Streak Virus Antigens with Those of Three Other Rhabdoviruses Infecting the Gramineae

Cited by 11 publications

References 3 publications

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus1)

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus1)

Serological Relationships between the Nucleocapsids of Some Planthopper-Borne Rhabdoviruses of Cereals

Ultrastructure of viruliferous Javesella pellucida transmitting Festuca leaf streak virus (genus Cytorhabdovirus)

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Product

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Purification and Serology of the Iranian Maize Mosaic Rhabdovirus¹)

Purification and Serology of the Iranian Maize Mosaic Rhabdovirus¹)