Comparison of Low- And High-Intensity Resistance Exercise on Lipid Peroxidation

Abstract

The purpose of this study was to examine the effect of low- vs. high-intensity resistance exercise on lipid peroxidation. In addition, the role of muscle oxygenation on plasma malondialdehyde (MDA) concentrations was explored. Eleven experienced resistance trained male athletes (age: 20.8 +/- 1.3 years; weight: 96.2 +/- 14.4 kg; height: 182.4 +/- 7.3 cm) performed 4 sets of the squat exercise using either a low-intensity, high-volume (LI; 15 repetitions at 60% 1 repetition maximum [1RM]) or high-intensity, low-volume (HI; 4 repetitions at 90% 1RM load). Venous blood samples were obtained before the exercise (PRE), immediately following the exercise (IP), and 20 (20P) and 40 minutes (40P) postexercise. Continuous wave near-infrared spectroscopy was used to measure muscle deoxygenation in the vastus lateralis during exercise. Deoxygenated Hb/Mb change was used to determine reoxygenation rate during recovery. No difference in MDA concentrations was seen between LI and HI at any time. Significant correlations were observed between plasma MDA concentrations at IP and the half-time recovery (T1/2 recovery) of muscle reoxygenation (r = 0.45) and between T1/2 recovery and the area under the curve for MDA concentrations (r = 0.44). Results suggest that increases in MDA occur independently of exercise intensity, but tissue acidosis may have a larger influence on MDA formation.

The Effect of Resistance Exercise on Humoral Markers of Oxidative Stress

“…Unfortunately, only one oxidative damage marker, malondialdehyde, was used, and no increases were elicited by either intensity. Nonetheless, when plasma malondialdehyde values were correlated with an indirect marker of tissue oxygenation, a modest but significant positive relationship resulted (11). This finding implicates a relationship between resistance exercise intensity and oxidative stress and further validates the study rationale of the current investigation.…”

“…Thus, despite intensity differences between the two protocols, the energy demand per minute was significantly greater during the hypertrophy protocol as compared with the strength protocol. In support, a recent investigation by Hoffman et al (11) examined blood oxidative stress following similar resistance exercise regimens of 60% 1RM and 90% 1RM. Unfortunately, only one oxidative damage marker, malondialdehyde, was used, and no increases were elicited by either intensity.…”

“…Hoffman et al (11) have performed a preliminary investigation of resistance exercise intensity and oxidative stress, though their findings are limited to a single blood oxidative damage marker (malondialdehyde). In light of the questionable value of this oxidative damage marker, further investigation is needed to better clarify the impact of exercise intensity on blood oxidative stress following resistance exercise of different intensities.…”

Six weeks of aerobic dance exercise improves blood oxidative stress status and increases interleukin-2 in previously sedentary women

“…In regards to this focal point, there was a significant reduction of MDA (from 2.28, 0.19 to 0.98, 0.22 mmol/L) and an improvement in the TAC (from 1.31, 0.08 to 2.58, 0.07 mmol Trolox/L). Many studies have previously examined the effects of acute or prolonged heavy intensity exercise with resulting high levels of MDA and lower TAC threshold compared to a baseline period (Hoffman et al, 2007). But in the present study, the intensity of exercise was moderate (75% of MHR; mean HR Z 149.4 (5.6) beats per min) and achieved with a moderate aerobic dance cadence of 70 steps per min for 30 min.…”

Exercise Improves Visual Deficits Tested by Visual Evoked Potentials in Streptozotocin-Induced Diabetic Rats

“…The other two groups were injected with an equivalent volume of citrate buffer. Three days later, the glucose concentration in the temperature (Sen 1995;Dekkers et al 1996;Hoffman et al 2007). …”