Comparison of lncRNA and mRNA expression in mouse brains infected by a wild-type and a lab-attenuated Rabies lyssavirus

Sui, Baokun; Chen, Dong; Liu, Wei; Tian, Bin; Pei, Jie; Wu, Qiong; Zhou, Ming; Fu, Zhen F.; Zhang, Yi; Zhao, Ling

doi:10.1099/jgv.0.001538

Cited by 9 publications

(5 citation statements)

References 71 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transcriptomics has been widely used to screen for potentially functional genes. Previous RABV-related transcriptomics studies have mainly focused on the nervous system, investigating changes in lncRNA, circRNA, and mRNA expression in the mouse brain caused by RABV infection ( Zhang et al, 2016b ; Sui et al, 2021 ; Zhao et al, 2021 ). The spatial and cellular distribution of RABV infection in the mouse brain has also been deeply revealed ( Huang and Sabatini, 2020 ; Zhang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of salivary glands of rabies-virus-infected mice

Guo,

Zhang,

Feng

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Rabies is a fatal zoonotic disease that poses a threat to public health. Rabies virus (RABV) is excreted in the saliva of infected animals, and is primarily transmitted by bite. The role of the salivary glands in virus propagation is significant, but has been less studied in the pathogenic mechanisms of RABV. To identify functionally important genes in the salivary glands, we used RNA sequencing (RNA-seq) to establish and analyze mRNA expression profiles in parotid tissue infected with two RABV strains, CVS-11 and PB4. The biological functions of differentially expressed genes (DEGs) were determined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, which revealed 3,764 DEGs (678 up-regulated and 3,086 down-regulated) in the CVS-11 infected group and 4,557 DEGs (874 up-regulated and 3,683 down-regulated) in the PB4 infected group. Various biological processes are involved, including the salivary secretion pathway and the phosphatidylinositol 3-kinase-Akt (PI3K-Akt) signaling pathway. This study provides the first mapping of the transcriptome changes in response to RABV infection in parotid tissue, offering new insights into the study of RABV-affected salivary gland function and RABV pathogenic mechanisms in parotid tissue. The salivary gland-enriched transcripts may be potential targets of interest for rabies disease control.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of salivary glands of rabies-virus-infected mice

Guo,

Zhang,

Feng

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…As anticipated, we observed a decline in the width and surface area of the ruminal papilla. Synchronously, LP inclusion resulted in the upregulation of PCP4 , which played a vital role in the procedure of pathological microbiota infection resistance (Ge, 2003 ; Sui et al, 2021 ). It is not surprising to find that CALD1 and TAGLN , which promoted inflammation, were upregulated in the LP group, implying an elevated inflammation risk in the GIT (Shen et al, 2010 ; Liu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The epithelial transcriptome and mucosal microbiota are altered for goats fed with a low-protein diet

Wu,

Tian,

Jiao

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionFeeding low protein (LP) diet to animals impose severe challenge to animals' immune homeostasis. However, limited knowledge about the underlying adaption mechanism of host and ruminal microbiota responding to LP diet were well understood. Herein, this study was performed to examine the changes in relative abundance of ruminal microbiota and host ruminal mucosal transcriptome profiles in response to a LP diet.MethodsA total of twenty-four female Xiangdong balck goats with similar weight (20.64 ± 2.40 kg) and age (8 ± 0.3 months) were randomly assigned into two groups, LP (5.52% crude protein containing diet) and CON (10.77% crude protein containing diet) groups. Upon completion of the trial, all goats were slaughtered after a 16-hour fasting period in LiuYang city (N 28°15′, E 113°63′) in China. HE staining, free amino acids measurement, transcriptome analysis and microbiome analysis were applied to detect the morphology alterations, free amino acids profile alterations and the shift in host ruminal mucosal transcriptome and ruminal microbiota communities.ResultsFirstly, the results showed that feeding LP diet to goats decreased the rumen papilla width (P = 0.043), surface area (P = 0.013) and total ruminal free amino acids concentration (P = 0.016). Secondly, microbiome analysis indicated that 9 microbial genera, including Eubacterium and Prevotella, were enriched in LP group while 11 microbial genera, including Butyrivibrio and Ruminococcus, were enriched in CON group. Finally, in terms of immune-related genes, the expression levels of genes involved in tight junction categories (e.g., MYH11, PPP2R2C, and MYL9) and acquired immunity (e.g., PCP4 and CXCL13) were observed to be upregulated in the LP group when compared to the CON group.ConclusionUnder the LP diet, the rumen exhibited increased relative abundance of pathogenic microbiota and VFA-degrading microbiota, leading to disruptions in immune homeostasis within the host's ruminal mucosa. These findings indicate that the ruminal microbiota interacts with host results in the disruption in animals' immune homeostasis under LP diet challenge.

show abstract

“…Once the virus enters the CNS, RABV triggers innate immune responses of neurons [256], astrocytes [109,144], and microglia [109,249], which mount primary (activation of NF-κB) and secondary IFN responses (activation of Janus kinase-signal transducers and activators of transcription (JAK-STAT)), inducing the production of ISGs, such as cytokines and chemokines [245]. However, the extent of immune responses triggered strongly depends on the cell type [109] and the viral strain used [114,122,144,267]. Cytokines and chemokines are crucial to recruit peripheral immune cells to the CNS, since both favor changes in BBB permeability, which in turn regulates the contact between circulating immune cells and the neural tissue [101].…”

Section: Rabies Virusmentioning

confidence: 99%

Innate Immune Signaling and Role of Glial Cells in Herpes Simplex Virus- and Rabies Virus-Induced Encephalitis

Feige

Zaeck

Sehl-Ewert

et al. 2021

Viruses

View full text Add to dashboard Cite

The environment of the central nervous system (CNS) represents a double-edged sword in the context of viral infections. On the one hand, the infectious route for viral pathogens is restricted via neuroprotective barriers; on the other hand, viruses benefit from the immunologically quiescent neural environment after CNS entry. Both the herpes simplex virus (HSV) and the rabies virus (RABV) bypass the neuroprotective blood–brain barrier (BBB) and successfully enter the CNS parenchyma via nerve endings. Despite the differences in the molecular nature of both viruses, each virus uses retrograde transport along peripheral nerves to reach the human CNS. Once inside the CNS parenchyma, HSV infection results in severe acute inflammation, necrosis, and hemorrhaging, while RABV preserves the intact neuronal network by inhibiting apoptosis and limiting inflammation. During RABV neuroinvasion, surveilling glial cells fail to generate a sufficient type I interferon (IFN) response, enabling RABV to replicate undetected, ultimately leading to its fatal outcome. To date, we do not fully understand the molecular mechanisms underlying the activation or suppression of the host inflammatory responses of surveilling glial cells, which present important pathways shaping viral pathogenesis and clinical outcome in viral encephalitis. Here, we compare the innate immune responses of glial cells in RABV- and HSV-infected CNS, highlighting different viral strategies of neuroprotection or Neuroinflamm. in the context of viral encephalitis.

show abstract

Comparison of lncRNA and mRNA expression in mouse brains infected by a wild-type and a lab-attenuated Rabies lyssavirus

Cited by 9 publications

References 71 publications

Transcriptome analysis of salivary glands of rabies-virus-infected mice

Transcriptome analysis of salivary glands of rabies-virus-infected mice

The epithelial transcriptome and mucosal microbiota are altered for goats fed with a low-protein diet

Innate Immune Signaling and Role of Glial Cells in Herpes Simplex Virus- and Rabies Virus-Induced Encephalitis

Contact Info

Product

Resources

About