Comparison of liposomal and 2-hydroxypropyl-β-cyclodextrin–lidocaine on cell viability and inflammatory response in human keratinocytes and gingival fibroblasts

Ferreira, Luiz Eduardo Nunes; Muniz, Bruno Vilela; Santos, Cleiton Pita dos; Volpato, Maria Cristina; Paula, Eneida de; Groppo, Francisco Carlos

doi:10.1111/jphp.12552

Cited by 7 publications

(4 citation statements)

References 47 publications

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“…Local anesthetics have been tested in different kind of cells, as and fibroblasts, neuroblastoma, mesenchymal stem, keratinocytes [18,[31][32][33]. The immortalized human keratinocytes (HaCaT) have a good proliferative capacity in culture, being used in in vitro studies of cytotoxicity [24,33,34] and other cellular responses to stimulus [35,36].…”

Section: Discussionmentioning

confidence: 99%

“…Cell culture. Immortalized human keratinocytes (HaCaT) were chosen due to their good proliferative capacity in culture and their use in previous in vitro cytotoxicity studies of local anesthetics and drug delivery systems [24,25]. The HaCaT cells (BCRJ 0100, Rio de Janeiro Cell Bank, Rio de Janeiro, RJ, Brazil) were grown in 75 cm 2 TPP 1 bottles (Techno Plastic Products AG, Trasadingen, Switzerland) containing Dulbecco's Modified Eagle Medium (DMEM, Vitrocell, Campinas, SP, Brazil) supplemented with 10% (v/v) fetal bovine serum (Vitrocell), 10,000 U/mL penicillin (Vitrocell), and 100 μg/mL streptomycin (Vitrocell), at 37˚C, in a humid atmosphere with 5% CO 2 [24,25] .…”

Section: In Vitro Modelsmentioning

confidence: 99%

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Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia

et al. 2021

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To determine whether the permeation capacity and analgesic efficacy of articaine (ATC) could be increased and cytotoxicity decreased by encapsulation in poly(ɛ-caprolactone) nanocapsules (ATCnano), aiming at local or topical anesthesia in dentistry. Cellular viability was evaluated (using the MTT test and fluorescence microscopy) after 1 h and 24 h exposure of HaCaT cells to ATC, ATCnano, ATC with epinephrine (ATCepi), and ATC in nanocapsules with epinephrine (ATCnanoepi). The profiles of permeation of 2% ATC and 2% ATCnano across swine esophageal epithelium were determined using Franz-type vertical diffusion cells. Analgesic efficacy was evaluated with a von Frey anesthesiometer in a postoperative pain model in rats, comparing the 2% ATC, 2% ATCnano, 2% ATCepi, and 2% ATCnanoepi formulations to 4% ATCepi (a commercially available formulation). We show that use of the nanocapsules decreased the toxicity of articaine (P<0.0001) and increased its flux (P = 0.0007). The 2% ATCepi and 4% ATCepi formulations provided higher analgesia success and duration (P<0.05), compared to 2% ATC, 2% ATCnano, and 2% ATCnanoepi. Articaine-loaded poly(ɛ-caprolactone) nanocapsules constitute a promising formulation for intraoral topical anesthesia (prior to local anesthetic injection), although it is not effective when injected in inflamed tissues for pain control, such as irreversible pulpitis.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Modelsmentioning

confidence: 99%

Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia

et al. 2021

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“…Immortalized human HaCaT cells have served as a valuable model in skin toxicology and wound healing studies (27)(28)(29)(30)(31)(32)(33). In addition, as the value of skin cultures as feasible cholesteatoma model have been acknowledged for several decades (34), HaCaT cells have provided a useful in vitro model in cholesteatoma research (35), thus complementing animal models and cell models using primary cholesteatoma keratinocytes (12,16,17,19).…”

Section: Discussionmentioning

confidence: 99%

The Viability and Growth of HaCaT Cells After Exposure to Bioactive Glass S53P4-Containing Cell Culture Media

Sarin

Vuorenmaa

Vallittu

et al. 2021

Otology &Amp; Neurotology

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Hypothesis: Bioactive glass (BG) S53P4 reduces the viability of epidermal keratinocyte-derived immortalized cell line, HaCaT in sufficient concentrations in vitro. Background: Although used in mastoid obliteration surgery, there is no data available on whether BG S53P4 granules have an inhibitory or excitatory effect on keratinocytes, found in normal skin and ear cholesteatoma in vivo. Methods: HaCaT cell cultures were incubated with a direct BG S53P4 granule contact. Microscopic evaluation of the cultures was performed and interleukin-6 (IL-6) and -8 (IL-8) concentrations were measured from the medium samples. In addition, BG granules were incubated in two cell culture media for 6 days and the pure media were used in confluent HaCaT cultures preceding cell viability assay. Finally, a scratch assay test was performed to reveal the possible BG effect on HaCaT cultures.Results: Eight to ten cell thick layers of dead HaCaT cells were noticed after a 2-day BG granule contact. With a BG concentration of 2.5%, IL-6 and IL-8 concentrations were smaller compared with the control group without BG after 2 days' incubation. Overall, HaCaT cell viability decreased when BG was incubated in keratinocyte growth medium, but did not change in Dulbecco's modified Eagle's medium. In a scratch assay test, cell regrowth in the scratch area was notable in cultures without BG. Conclusions: BG S53P4 seems to have an inhibitory effect on HaCaT cell growth. Although further studies are needed, this observation seems advantageous for cholesteatoma treatment.

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“…A citotoxicidade de AL sobre diferentes tipos celulares já é bem descrita na literatura (ARAÚJO et al, 2005;GRILLO et al, 2010). A citotoxicidade está relacionada a lipossolubilidade do AL, concentração e tempo de exposição (FERREIRA et al, 2016;KROG et al, 2002;RADWAN;SAITO;GOTO, 2002;YANG et al, 2011). Em cultura primária de Em relação a morfologia celular, a Figura 8A mostra uma imagem representativa (controle) da linhagem de CS estudada, revelando células esféricas e com prolongamento citoplasmático, em conformidade com seu padrão morfológico normal ("RSC96 cell line").…”

Section: Análise Da Expressão De Proteínas Por Western Blottingunclassified

Articaína

Henrique Rodrigues da Silva

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Comparison of liposomal and 2-hydroxypropyl-β-cyclodextrin–lidocaine on cell viability and inflammatory response in human keratinocytes and gingival fibroblasts

Abstract: The effect of drug carrier in the lidocaine effects was dependent on the cell type, concentration and time of exposure. MLV and HP-β-CD showed benefits in improving cell viability; however, both of them showed a tendency to increase cytokine release when compared to the plain solution.

Cited by 7 publications

References 47 publications

Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia

Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia

The Viability and Growth of HaCaT Cells After Exposure to Bioactive Glass S53P4-Containing Cell Culture Media

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