Comparison of lipid profiles of Malassezia pachydermatis strains isolated from dogs with otitis externa and without clinical symptoms of disease

Abstract

Malassezia pachydermatis can cause infections of the skin and mucous membranes, especially in animals. It becomes a problem also in medicine. It is considered that metabolic disorders as well as hormonal and immunological status of the host promote diseases caused by M. pachydermatis. Here we consider whether specific features of fungi could also favour infections. We checked whether there are differences in lipid profiles between strains obtained from dogs with otitis externa and strains obtained from healthy dogs. Lipid profiles were determined using thin layer chromatography and gas chromatography-mass spectrometry. All analyses were carried out on 32 strains derived from dogs with otitis externa and 31 strains isolated from dogs without symptoms of disease. The results show that strains isolated from dogs without symptoms of otitis externa are characterised by a higher content of fatty acids. They contain significantly more behenic and lignoceric acids on medium without addition of lipids, and more oleic acid and total monounsaturated fatty acids on medium with lipids supplementation. These strains have also a higher content of esters of ergosterol and triglycerides. Data obtained show differences which may be specific features of M. pachydermatis-specific strains related to the ability of infection, which could be not directly related of the host condition.

An experimental murine model of otitis and dermatitis caused by Malassezia pachydermatis

“…Tragiannidis et al described that Malassezia species have a strengthened cell wall, fundamental for stimulate the reticuloendothelial system and activate the complement cascade but also suppress cytokine release and downregulate phagocytic uptake and killing. However, Czyzewska et al recently described that the ability of infection may be related to specific features of the fungus and not directly related to the host condition.…”

Evaluation of the effect of a lysozyme-chitosan sugar complex (LYZOX) and decanoic acid spray on local lesions of nine dogs suspected of Malassezia dermatitis

“…1) Bayside Animal Hospital, 2) Wako Filter Technology, 3) Teikyo University Malassizia pachydermatis 4,8,21) Malassezia spp.…”

Back to the Basics: Two Approaches for the Identification and Extraction of Lipid Droplets from Malassezia pachydermatis CBS1879 and Malassezia globosa CBS7966

“…Malassezia spp. are lipophilic and lipid‐dependent yeasts associated with skin mycobiota of human and animals (Cabañes, 2014; Chang et al., 1998; Czyzewska et al., 2016). This genus includes 18 species (Lorch et al., 2018) associated with skin disorders (Cabañes, 2014; Grice & Dawson Jr., 2017; Hay & Midgley, 2010), fungemia in neonates and immunocompromised patients (Cabañes, 2014; Guého‐Kellermann, Boekhout, & Begerow, 2010; Hay & Midgley, 2010), and more recently Crohn's disease, exacerbation of colitis, Parkinson disease, and pancreatic ductal adenocarcinoma (Aykut et al., 2019; Laurence, Benito‐León, & Calon, 2019; Limon et al., 2019).…”