Comparison of laser-assisted hatching and acidified Tyrode’s hatching by evaluation of blastocyst development rates in sibling embryos: a prospective randomized trial

Jones, Anny Brooksbank; Wright, Graham; Kort, Hilton I.; Straub, R.J.; Nagy, Zsolt

doi:10.1016/j.fertnstert.2005.07.1314

Cited by 45 publications

(24 citation statements)

References 24 publications

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“…Previous research used the rate of lysed blastomeres to describe the efficiency of the embryo biopsy procedure [12,13]. In the current study, the variation of the pulse length and the resultant hole did not influence the rate of lysed blastomeres.…”

Section: Discussionmentioning

confidence: 49%

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Taylor

Gilchrist

Hallowell

et al. 2010

J Assist Reprod Genet

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Purpose A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development. Methods Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III). Results For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0±0.0), II (1.0±0.2), and III (1.1±0.2) was significant (0.0001). Conclusion Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.

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Section: Discussionmentioning

confidence: 49%

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Taylor

Gilchrist

Hallowell

et al. 2010

J Assist Reprod Genet

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“…Independently from the procedure chosen, a biopsy involves two steps: 1) zona pellucida (ZP) drilling, which can be performed mechanically, chemically or by laser energy [18][19][20], and 2) cell/s removal.…”

Section: Biopsymentioning

confidence: 99%

Preimplantation genetic diagnosis and the biopsy technique: Important considerations

Greco¹,

Fabozzi²,

Ruberti³

et al. 2013

ARSci

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Preimplantation genetic diagnosis allows to test the genetic status of embryos prior to implantation. In order to obtain genetic material, on which carry out a genetic diagnosis, a procedure named embryo biopsy is required. In the last two decades, embryo biopsy at the cleavage stage has been the mostly performed procedure. However, recently, alternative methods allowing the retrieval of a larger number of cells (blastocyst stage biopsy), or representing a valid alternative to overcome ethical issues (polar body biopsy) have obtained increasing consensus. This article reviews different methods of embryo biopsy and points out their positive and negative aspects.

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“…The 1.48 micron infrared diode laser beam has been more widely used in clinical IVF labs as an efficient and simple method for embryo hatching. Multiple studies have demonstrated its safety (Sagoskin et al, 2007;Lanzendorf et al, 2007;Wong et al, 2003) as well as efficacy when compared to acid hatching (Lanzendorf et al, 2007;Balaban et al, 2002;Jones et al, 2006). The optimal technique for laser assisted hatching is still being debated.…”

Section: Assisted Hatchingmentioning

confidence: 99%

“…These investigators concluded that all methods were comparable based on the outcome parameters studied, including implantation and pregnancy rates, multiple pregnancy rates and abortion rates (Balaban et al, 2002). Additional studies comparing laser assisted hatching with acid drilling showed no significant differences with respect to pregnancy rates (Lanzendorf et al, 2007;Jones et al, 2006). Laser assisted hatching is generally well-accepted in IVF labs, allowing improved standardization between operators (Lanzendorf et al, 2007;Jones et al, 2006).…”

Section: Assisted Hatchingmentioning

confidence: 99%

“…Additional studies comparing laser assisted hatching with acid drilling showed no significant differences with respect to pregnancy rates (Lanzendorf et al, 2007;Jones et al, 2006). Laser assisted hatching is generally well-accepted in IVF labs, allowing improved standardization between operators (Lanzendorf et al, 2007;Jones et al, 2006). Children followed to one year of age after an assisted pregnancy using laser assisted hatching were Fig.…”

Section: Assisted Hatchingmentioning

confidence: 99%

See 1 more Smart Citation

Laser Pulse Application in IVF

Bedient¹,

Khanna²,

Desai³

2011

Lasers - Applications in Science and Industry

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Comparison of laser-assisted hatching and acidified Tyrode’s hatching by evaluation of blastocyst development rates in sibling embryos: a prospective randomized trial

Cited by 45 publications

References 24 publications

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Preimplantation genetic diagnosis and the biopsy technique: Important considerations

Laser Pulse Application in IVF

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