Comparison of Lactate Dehydrogenase Activity in Hive and Forager Honeybees May Indicate Delayed Onset Muscle Soreness – Preliminary Studies

Strachecka, Aneta; Grzybek, Maciej; Ptaszyńska, Aneta A.; Łoś, Aleksandra; Chobotow, Jacek; Rowiński, Rafał

doi:10.1134/s0006297919040114

Cited by 4 publications

(4 citation statements)

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“…For this reason, LDH is used as one of several relevant parameters to assess the severity of COVID-19 (Zhang et al, 2020b). The augmented levels of LDH in COVID-19 are related to an increase in the production of lactate, which means that changes in the glycolytic pathway and/or low oxygen conditions occur in cells in the context of infection and/or activation (Ding et al, 2017;Strachecka et al, 2019;Farhana and Lappin, 2020;Morassi et al, 2020). In SARS-CoV 2 infection, with abrupt lung involvement, there is a decrease in oxygen supply in lung cells due to inflammatory events resulting from the infection (Jahani et al, 2020;Serebrovska et al, 2020;Ye et al, 2020) This situation leads to a set of changes in the glycolytic pathway, as well as increased glucose uptake, that impair cell function, thereby worsening events that eventually lead to multiorgan dysfunction, as increased glucose uptake promotes ideal conditions for an inflammatory environment and ROS production (Paces et al, 2020).…”

Section: Increased Glucose Consumption By Cells In Sars-cov-2 Infectionmentioning

confidence: 99%

Changes in Glycolytic Pathway in SARS-COV 2 Infection and Their Importance in Understanding the Severity of COVID-19

et al. 2021

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COVID-19 is an infectious disease caused by Coronavirus 2 (SARS-CoV-2) that may lead to a severe acute respiratory syndrome. Such syndrome is thought to be related, at least in part, to a dysregulation of the immune system which involves three main components: hyperactivity of the innate immune system; decreased production of type 1 Interferons (IFN) by SARS-CoV-2-infected cells, namely respiratory epithelial cells and macrophages; and decreased numbers of both CD4+ and particularly CD8+ T cells. Herein, we describe how excessive activation of the innate immune system and the need for viral replication in several cells of the infected organism promote significant alterations in cells’ energy metabolism (glucose metabolism), which may underlie the poor prognosis of the disease in severe situations. When activated, cells of the innate immune system reprogram their metabolism, and increase glucose uptake to ensure secretion of pro-inflammatory cytokines. Changes in glucose metabolism are also observed in pulmonary epithelial cells, contributing to dysregulation of cytokine synthesis and inflammation of the pulmonary epithelium. Controlling hyperglycolysis in critically ill patients may help to reduce the exaggerated production of pro-inflammatory cytokines and optimise the actions of the adaptive immune system. In this review, we suggest that the administration of non-toxic concentrations of 2-deoxy-D-glucose, the use of GLUT 1 inhibitors, of antioxidants such as vitamin C in high doses, as well as the administration of N-acetylcysteine in high doses, may be useful complementary therapeutic strategies for these patients, as suggested by some clinical trials and/ or reports. Overall, understanding changes in the glycolytic pathway associated with COVID-19 infection can help to find new forms of treatment for this disease.

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Section: Increased Glucose Consumption By Cells In Sars-cov-2 Infectionmentioning

confidence: 99%

Changes in Glycolytic Pathway in SARS-COV 2 Infection and Their Importance in Understanding the Severity of COVID-19

et al. 2021

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“…This suggests Zn-fed steers were experiencing less muscle fatigue than CON after the transit event. Lactate is cleared from the muscle and taken to the liver, where it is converted back to pyruvate ( Sharma et al, 2019 ; Strachecka et al, 2019 ) via the Zn-dependent enzyme lactate dehydrogenase B (LDH; Price, 1962 ). Pyruvate can then enter gluconeogenesis to produce glucose, which could return to the muscle for ATP production.…”

Section: Discussionmentioning

confidence: 99%

“…Lactate is a valuable biomarker of muscle fatigue and is created from pyruvate, which is an anaerobic ATP production end product ( Van Hall, 2010 ). Before entering the Cori Cycle pathway to support the energy demands of muscle during exercise ( Sharma et al, 2019 ; Strachecka et al, 2019 ), lactate can be converted back into pyruvate via the Zn-dependent enzyme lactate dehydrogenase ( Price, 1962 ). As such, a practical solution to improve the transition into the feedlot may be increasing the dietary Zn concentration of calves before long-duration transportation to the feedlot.…”

Section: Introductionmentioning

confidence: 99%

Effect of increasing zinc supplementation on post-transit performance, behavior, blood and muscle metabolites, and gene expression in growing beef feedlot steers

Heiderscheit

Hansen

2022

Journal of Animal Science

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Fifty-four Angus-cross steers (297 kg ± 12) were stratified by body weight (BW) to pens (6 steers per pen) to determine effects of supplemental Zn on post-transit growth performance and blood and muscle metabolites. Dietary treatments started 25 d before trucking: control (CON; analyzed 54 mg Zn/kg DM), industry (IND; CON + 70 mg supplemental Zn/kg DM), and supranutritional Zn (SUPZN; CON + 120 mg supplemental Zn/kg DM). Supplemental Zn was bis-glycinate bound Zn (Plexomin Zn; Phytobiotics North America, Cary, NC). On d 0, steers were loaded onto a commercial trailer and transported 18 h (1,822 km). Individual BW were recorded on d -26, -25, -1, 0 (pre-transit), 1 (post-transit), 6, 27, and 28. Blood was collected on d -1, 1, 6, and 27. Longissimus thoracis biopsies were collected on d -1, 1, and 28. Daily individual feed disappearance was recorded via GrowSafe bunks. Data were analyzed using Proc Mixed of SAS with fixed effect of diet and steer as the experimental unit (growth performance, blood: n = 18 steers per treatment; muscle: n = 12 steers per treatment). Individual initial BW was used as a covariate in BW analysis. Contrast statements to test linear, quadratic, and Zn effects were used to analyze performance and blood parameters. Repeated measures analysis was used for post-transit DMI recovery and weekly post-transit DMI and Zn intake with the repeated effect of time. MetaboAnalyst 5.0 was utilized for statistical analysis of d 1 (off truck) muscle metabolites. Plasma Zn linearly increased due to Zn on d 1, 6, and 27 (P = 0.01), and off-truck (d 1) serum lactate increased over d -1 by 20, 0, and 20% in CON, IND, and SUPZN, respectively (Quadratic: P = 0.01). Muscle lactate tended to increase post-transit in CON and IND (P ≤ 0.07) but not SUPZN. Muscle metabolites relating to amino acid and nitrogen metabolism were increased in all treatments post-transit (P ≤ 0.02), and alanine-glucose cycle metabolites tended to increase in CON and IND (P ≤ 0.07). Steers supplemented with Zn recovered pre-transit DMI quicker than CON (by d 2: P = 0.01), while IND had greater overall post-transit DMI than CON with SUPZN intermediate (P = 0.04), and Zn-fed steers had greater ADG post-transit (P = 0.04). Zinc supplementation mitigated muscle or serum lactate increases due to transit and increased post-transit ADG.

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“…Lactate is produced as a byproduct of anaerobic metabolism, and it can be converted back into pyruvate in a process called the Cori cycle. [46][47][48] Furthermore, cells can actively uptake lactate, channeling it into mitochondria for oxidation, subsequently generating ATP. 49 This mechanism is recognized as the 'lactate shuttle', gaining particular significance in tissues characterized by elevated energy requirements, like cardiac tissue skeletal and muscle.…”

Section: Nad(p) Detection In Live Cellsmentioning

confidence: 99%

Sensitive monitoring of NAD(P)H levels within cancer cells using mitochondria-targeted near-infrared cyanine dyes with optimized electron-withdrawing acceptors

Arachchige,

Dwivedi,

Waters

et al. 2024

J. Mater. Chem. B

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Comparison of Lactate Dehydrogenase Activity in Hive and Forager Honeybees May Indicate Delayed Onset Muscle Soreness – Preliminary Studies

Cited by 4 publications

References 26 publications

Changes in Glycolytic Pathway in SARS-COV 2 Infection and Their Importance in Understanding the Severity of COVID-19

Changes in Glycolytic Pathway in SARS-COV 2 Infection and Their Importance in Understanding the Severity of COVID-19

Effect of increasing zinc supplementation on post-transit performance, behavior, blood and muscle metabolites, and gene expression in growing beef feedlot steers

Sensitive monitoring of NAD(P)H levels within cancer cells using mitochondria-targeted near-infrared cyanine dyes with optimized electron-withdrawing acceptors

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