Comparison of label-free and GFP multiphoton imaging of hair follicle-associated pluripotent (HAP) stem cells in mouse whiskers

Uchugonova, Aisada; Cao, Wenluo; Hoffman, Robert M.; Koenig, Karsten

doi:10.1080/15384101.2015.1090064

Cited by 5 publications

(3 citation statements)

References 18 publications

(28 reference statements)

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“…The PMT1924 photodetector is used to detect signals from both fluorescence and SHG channels. Multiphoton tomography can used for noninvasive imaging of GFP-expressing cells or to image cells in vivo based on their intrinsic autofluorescence (Uchugonova, Cao, Hoffman, & Koenig, 2015;Uchugonova et al, 2013).…”

Section: Multiphoton Laser-scanning Microscopymentioning

confidence: 99%

The Advantages of Using Fluorescent Proteins for In Vivo Imaging

Hoffman

2017

CP Essential Lab Tech

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Fluorescent proteins have revolutionized biology by enabling what was formerly invisible to be seen clearly, and potentially enable imaging of all cells in living animals. There are numerous applications for in vivo imaging with fluorescent proteins, including use in cancer, immunology, infection, and neurobiology studies. This unit describes how introducing the GFP and RFP gene into malignant tissue has enabled imaging of primary cancer and the metastatic process with subcellular resolution in vivo. © 2017 by John Wiley & Sons, Inc.

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Section: Multiphoton Laser-scanning Microscopymentioning

confidence: 99%

The Advantages of Using Fluorescent Proteins for In Vivo Imaging

Hoffman

2017

CP Essential Lab Tech

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“…Hair follicles contain a distinct population of follicular stem cells that express Nestin (15). Using Nestin-green fluorescent protein (GFP) transgenic mice, researchers demonstrated that during telogen and in early anagen, Nestin-GFP + cells are primarily in the bulge area (9,16). However, in mid-and late anagen, the GFP-expressing cells are located in the upper outer-root sheath in addition to the bulge area (9).…”

Section: Introductionmentioning

confidence: 99%

Dynamic Nestin expression during hair follicle maturation and the normal hair cycle

2018

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Nestin, a type-VI intermediate filament protein, serves as a marker for neural stem cells, and is also known to be expressed in follicle stem cells. Hair follicles go through repeated cycles of anagen (growth), catagen (regression) and telogen (quiescence) throughout the life of mammals following morphogenesis. In the present study it was demonstrated that in mice, the maturation of hair follicles includes the period between morphogenesis and the first anagen (4 weeks of age). Skin samples from Nestin-green fluorescent protein (GFP) + mice at different hair follicle stages were collected, and immunostaining for Nestin and Ki67 was performed. It was identified that during morphogenesis, Nestin-GFP expression was rarely detected and it gradually increased during maturation (0-4 weeks) in hair follicle dermal cells. In mature hair follicle dermal cells, Nestin and the proliferation marker Ki67 were highly expressed in anagen, while during telogen, they were markedly decreased. Additionally, lineage tracing data demonstrated that peri-follicular Nestin + cells during morphogenesis differentiated into cluster of differentiation 31 + cells.

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“…In the nonlinear interaction between incident photons and molecules in tissue, non-centrosymmetric molecules such as collagen, myosin, and tubulin generate light with half the wavelength of the excitation source. In the last two decades, SHG imaging has been used not only for characterization of normal collagenous tissues [11] , [12] , but also for diagnostic evaluation of various fibrotic diseases [13] , [14] , [15] . Recently, several groups applied SHG imaging to staging of liver fibrosis in human biopsied samples [16] , [17] .…”

Section: Introductionmentioning

confidence: 99%

Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)

Yamamoto

Oshima

Saitou

et al. 2016

Biochemistry and Biophysics Reports

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Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively.

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Comparison of label-free and GFP multiphoton imaging of hair follicle-associated pluripotent (HAP) stem cells in mouse whiskers

Cited by 5 publications

References 18 publications

The Advantages of Using Fluorescent Proteins for In Vivo Imaging

The Advantages of Using Fluorescent Proteins for In Vivo Imaging

Dynamic Nestin expression during hair follicle maturation and the normal hair cycle

Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)

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