Comparison of L-selectin and E-selectin ligand specificities: The L-selectin can bind the E-selectin ligands Sialyl Lex and Sialyl Lea

Berg, Ellen L.; Magnani, John L.; Warnock, R A; Robinson, Martyn K.; Butcher, Eugene C.

doi:10.1016/0006-291x(92)90697-j

Cited by 173 publications

(84 citation statements)

References 34 publications

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“…sialyl-Lewis x (sLe X )] on discrete macromolecules [2]. CLA + T cells are detected most notably by the mAb HECA-452, which binds glycans such as sLe X [3][4][5]. CLA + T cells can be stained by soluble recombinant E-selectin-immunoglobulin chimeric proteins, which are comprised of the extracellular region of E-selectin and the Fc region of an immunoglobulin (E-selectin/Fc)] [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)+ CD4+ T cells

Walcheck

2007

Immunology Letters

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SUMMARYT cells utilize the vascular adhesion molecule E-selectin to enter inflamed skin. T lymphocytes identified by the mAb HECA-452 [cutaneous lymphocyte-associated antigen (CLA) T cells] are enriched in E-selectin ligand expressing cells. However, the proportion of CLA + T cells reactive with an E-selectin/Fc chimeric construct, as determined by flow cytometry, can vary considerably between studies. One important variable in these studies has been the E-selectin/Fc chimeric used to assess ligand expression. We therefore compared the reactivity of mouse, rat, and human E-selectin/Fc from the same widely used commercial source with peripheral blood CLA + CD4 + T cells, neutrophils, and the promyelocytic cell line HL-60 by flow cytometry and by shear flow assays. We observed that the binding activities of the different E-selectin/Fc chimeras were considerably different. Mouse E-selectin/Fc demonstrated the highest binding activity with human neutrophils and HL-60 cells by both assay approaches, whereas human E-selectin/Fc demonstrated the lowest binding activity. In addition, mouse E-selectin/Fc binding increased essentially in a linear manner with increasing expression of CLA by CD4 + T cells, whereas human and rat E-selectin/Fc binding occurred with only a subset of CLA + CD4 + T cells. We conclude that there is substantial variability in the reactivity of different E-selectin/Fc constructs, thus caution should be used when assessing E-selectin ligand expression with these reagents. For instance, the discordance in expression of CLA and E-selectin ligands by T cells may in part be due to the E-selectin/Fc construct being used.

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Section: Introductionmentioning

confidence: 99%

Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)+ CD4+ T cells

Walcheck

2007

Immunology Letters

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“…The former mediates binding to thrombin activated platelets (23) while the latter permits attachment to E-selectin transfectants and cytokine-treated HUVE (24). These ligands have not been characterized but are presumably related to the sialylated, fucosylated binding sites identified on neutrophils and cell lines (25)(26)(27)(28)(29)(30)(31). The j31 integrin VLA-4 (CD49d) is constitutively expressed, enabling monocytes to interact with a second, cytokine-induced endothelial adhesion molecule termed VCAM-1 (24).…”

Section: Introductionmentioning

confidence: 99%

Monocyte-endothelial adhesion in chronic rheumatoid arthritis. In situ detection of selectin and integrin-dependent interactions.

Grober¹,

Bowen²,

Ebling³

et al. 1993

J. Clin. Invest.

146

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Blood monocytes are the principal reservoir for tissue macrophages in rheumatoid synovitis. Receptor-mediated adhesive interactions between circulating cells and the synovial venules initiate recruitment. These interactions have been studied primarily in cultured endothelial cells. Thus the functional activities of specific adhesion receptors, such as the endothelial selectins and the leukocytic integrins, have not been evaluated directly in diseased tissues. We therefore examined monocytemicrovascular interactions in rheumatoid synovitis by modifying the Stamper-Woodruff frozen section binding assay initially developed to study lymphocyte homing. Specific binding of monocytes to venules lined by low or high endothelium occurred at concentrations as low as 5 X 195 cells/ml. mAbs specific for P-selectin (CD62, GMP-140/PADGEM) blocked adhesion by > 90% in all synovitis specimens examined. In contrast, P-selectin-mediated adhesion to the microvasculature was either lower or absent in frozen sections of normal foreskin and placenta. mAbs specific for E-selectin (ELAM-1) blocked 20-50% of monocyte attachment in several RA synovial specimens but had no effect in others. mAbs specific for LFA-1, Mol /Mac 1, the integrin fl2-chain, and L-selectin individually inhibited 30-40% of adhesion. An mAb specific for the integrin Bl-chain inhibited the attachment ofelutriated monocytes up to 20%. We conclude that P-selectin associated with the synovial microvasculature initiates shear-resistant adhesion of monocytes in the Stamper-Woodruff assay and stabilizes bonds formed by other selectins and the integrins. Thus the frozen section binding assay permits direct evaluation of leukocytemicrovascular adhesive interactions in inflamed tissues and suggests a prominent role for P-selectin in monocyte recruitment in vivo. (J. Clin. Invest. 1993.91:2609-2619

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“…FucT-III, FucT-IV, FucT-V, FucT-VI, and FucT-IX can synthesize the Le x structure, while FucT-VII cannot. FucT-III, FucT-IV, FucT-V, FucT-VI, and FucT-VII can synthesize the sialyl Le x structure, which is the minimal ligand for all three selectins known as cell adhesion molecules for leukocyte-endothelium interaction (5,13,31). Detailed analysis of the substrate specificity of the polylactosamine acceptor has revealed Fuc-TIX to have more-efficient activity for synthesis of the Le x carbohydrate epitope than other ␣1,3FUTs (34) and to fucosylate the remote internal N-acetyllactosamine units of ␣2,3-sialylated polylactosamine (47).…”

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confidence: 99%

Normal Embryonic and Germ Cell Development in Mice Lacking α1,3-Fucosyltransferase IX (Fut9) Which Show Disappearance of Stage-Specific Embryonic Antigen 1

Kudo

Kaneko

Iwasaki

et al. 2004

Molecular and Cellular Biology

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Stage-specific embryonic antigen 1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during the 8-cell to blastocyst stages in mouse embryos and in primordial germ cells, undifferentiated embryonic stem cells, and embryonic carcinoma cells. For many years, SSEA-1 has been implicated in the development of mouse embryos as a functional carbohydrate epitope in cell-to-cell interaction during morula compaction. In a previous study, ␣1,3-fucosyltransferase IX (Fut9) exhibited very strong activity for the synthesis of Lewis x compared to other ␣1,3-fucosyltransferases in an in vitro substrate specificity assay. Fut4 and Fut9 transcripts were expressed in mouse embryos. The Fut9 transcript was detected in embryonic-day-13.5 gonads containing primordial germ cells, but the Fut4 transcript was not. In order to identify the role of SSEA-1 and determine the key enzyme for SSEA-1 synthesis in vivo, we have generated Fut9-deficient (Fut9 ؊/؊ ) mice. Fut9 ؊/؊ mice develop normally, with no gross phenotypic abnormalities, and are fertile. Immunohistochemical analysis revealed an absence of SSEA-1 expression in early embryos and primordial germ cells of Fut9 ؊/؊ mice. Therefore, we conclude that expression of the SSEA-1 epitope in the developing mouse embryo is not essential for embryogenesis in vivo.

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Comparison of L-selectin and E-selectin ligand specificities: The L-selectin can bind the E-selectin ligands Sialyl Lex and Sialyl Lea

Cited by 173 publications

References 34 publications

Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)+ CD4+ T cells

Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)+ CD4+ T cells

Monocyte-endothelial adhesion in chronic rheumatoid arthritis. In situ detection of selectin and integrin-dependent interactions.

Normal Embryonic and Germ Cell Development in Mice Lacking α1,3-Fucosyltransferase IX (Fut9) Which Show Disappearance of Stage-Specific Embryonic Antigen 1

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