Comparison of ISSR, IRAP and REMAP markers for assessing genetic diversity in different species of Brassica sp.

Mahjoob, B.; Zarini, Hamid Najafi; Hashemi, Seyyed Hamidreza; Shamasbi, Fatemeh Vafadar

doi:10.1134/s1022795416120073

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…The total mean of band frequency was 0.53 ranged from 0.5 to 0.6, the lowest values of mean band frequency were produced of primers (SCoT-01, while, the highest values of mean band frequency were produced of primers (SCoT-02, SCoT-04 and SCoT-21) these results agreed with Das et al (2014) who indicated that the use of ten primers amplified a total number of 353 bands under 93 loci through 5 mung bean genotypes with an average of 9.3 loci/primer appearing in general polymorphism of 52.7%. However, Mahjoob et al (2016) also showed that higher 𝑀𝐼 value (6.4) than our study using 13 ISSR markers for 35 Brassica genera. The difference between 𝑀𝐼 values with respect to previous studies could be because of different accessions and primers used (Akçali-Giachino, 2020).…”

Section: -+ ---+ + -contrasting

confidence: 63%

Histological Studies of some Brassicaceous Samples and the Genetic Variability Analysis using SCOT Markers

El-Shaht

El-Hefnawy

Hamza

et al. 2022

Journal of Agricultural Chemistry and Biotechnology

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Brassicaceae family (Cruciferae) known as mustard family, it is one of the largest families of flowering plants which have 372 genera and 4060 species all around the world. This study aims to investigate comparatively the anatomical structure of some plant samples of this family. Anatomical sections were taken on both stems and leaves, and 42 different qualitative characters were recorded in a data matrix. The results were numerically analysis to know the similarity between these samples by draw a dendrogram. Then the anatomical results were used to determine the genetics relationships between studied cultivated genera and the wild genera in order to includ in the breeding and improvement programs. Scot methods was chosen as it is fast, effective and gives accurate results. Start codon targeted (SCoT) polymorphism are one of the common markers were used to assess genetic diversity in some genera in Brassicaceae family. Seventin genera of the Brassicaceae have been used with ten SCoT primers. Totally, 174 bands were scored out of which 84.5% showed polymorphism, where 149 polymorphic bands and 25 monomorphic bands were observed. The results indicated that the existence of a significant degree of genetic similarity ranged from 0.62 to 0.88, where the highest mean value was 0.88 between genera 12 (Morettia canscens) and 13 (Moricandia siniaca) and the lowest mean value was 0.62 between genera 2 (Brassica napus) and 15 (Sinapis alba).

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confidence: 63%

Histological Studies of some Brassicaceous Samples and the Genetic Variability Analysis using SCOT Markers

El-Shaht

El-Hefnawy

Hamza

et al. 2022

Journal of Agricultural Chemistry and Biotechnology

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“…Molecular analysis using REMAP and ISSR markers is an efficient and inexpensive method to evaluate genetic diversity that has been used repeatedly to determine genetic relationships of many plants (Mahjoob et al 2016;Ghufaili and Al-Tamimi 2017;Cheraghi et al 2018;Vuorinen et al 2018). REMAP markers generate a wide range of band patterns.…”

Section: Discussionmentioning

confidence: 99%

“…REMAP markers generate a wide range of band patterns. They enable identification of polymorphisms between species as well as within a single species or cultivars (Kalendar et al 1999;Branco et al 2007;Mahjoob et al 2016;Cheraghi et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Molecular diversity analysis of genotypes from four Aegilops species based on retrotransposon–microsatellite amplified polymorphism (REMAP) markers

Leśniowska-Nowak

Okoń

Wieremczuk

2020

CEREAL RESEARCH COMMUNICATIONS

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Genetic diversity analysis is an important tool in crop improvement. Species with high genetic diversity are a valuable source of variation used in breeding programs. The aim of this study was to assess the genetic diversity of four species belonging to the genus Aegilops, which are often used to expand the genetic variability of wheat and triticale. Forty-five genotypes belonging to the genus Aegilops were investigated. Within- and among-species genetic diversity was calculated based on REMAP (retrotransposon–microsatellite amplified polymorphism) molecular markers. Obtained results showed that REMAP markers are a powerful method for genetic diversity analysis, which produces a high number of polymorphic bands (96.09% of total bands were polymorphic). Among tested genotypes, Ae. crassa and Ae. vavilovii showed the highest genetic diversity and should be chosen as a valuable source of genetic variation.

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“…ISSRs are generally very effective in genetic diversity studies because of their longer primers compared with RAPD primers (Pradeep Reddy et al, 2002;Tarıkahya-Hacıoğlu, 2016). Likewise, Mahjoob et al (2016) compared different markers such as ISSR, IRAP, and REMAP for the assessment of the genetic diversity within the Brassicaceae family. They reported that ISSR markers showed to be the most e cient for genetic diversity evaluations.…”

Section: Introductionmentioning

confidence: 99%

First report of genetic relationship and diversity among Moroccan and introduced rapeseed (Brassica napus l.) varieties as revealed by molecular markers

Houmanat¹,

Nabloussi²,

Rhazlaoui

et al. 2022

Preprint

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Rapeseed (Brassica napus L.) crop can be a lever for the development of oilseed sector in Morocco due to its adaptation to local conditions and its major economic and food importance. Breeding is the key to the success of this crop and judicious choice of crossing parents is a prerequisite to the success of the selection program and the development of new varieties that perform better than the old ones. In this regard, genetic variation within the existing germplasm must be explored and characterized. Therefore, the present study was carried out to investigate the genetic diversity among 22 varieties from Morocco as well as other origins, using twenty ISSR primers. The selected primers have generated a total of 319 markers. Polymorphic amplified bands varied from 8 to18, with an average of 13 per primer. The diversity index (PIC) ranged from 0.295 to 0.509, with a mean value of 0.37 per primer, indicating a good genetic diversity level for the primers used. The average similarity coefficient was 0.31, fluctuating between 0.176 and 0.456, and the pairwise comparison of the studied varieties showed a great discriminating power of primers and a large genetic diversity among accessions. Hierarchical classification allowed identifying three groups with some phylogeographic structuring. This is the first report of molecular characterization of rapeseed germplasm in Morocco and Africa. The obtained results have important implications for management of this germplasm to conserve the existing genetic diversity and use it properly in breeding programs in Morocco as well as in other Mediterranean and African countries.

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Comparison of ISSR, IRAP and REMAP markers for assessing genetic diversity in different species of Brassica sp.

Cited by 6 publications

References 32 publications

Histological Studies of some Brassicaceous Samples and the Genetic Variability Analysis using SCOT Markers

Histological Studies of some Brassicaceous Samples and the Genetic Variability Analysis using SCOT Markers

Molecular diversity analysis of genotypes from four Aegilops species based on retrotransposon–microsatellite amplified polymorphism (REMAP) markers

First report of genetic relationship and diversity among Moroccan and introduced rapeseed (Brassica napus l.) varieties as revealed by molecular markers

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