Comparison of iSED and Ves‐Matic Cube 200 Erythrocyte Sedimentation Rate Measurements With Westergren Method

Boğdaycıoğlu, Nihal; Yılmaz, Fatma Meriç; Sezer, Sevilay; Oğuz, Esra Fırat

doi:10.1002/jcla.21786

Cited by 25 publications

(41 citation statements)

References 8 publications

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“…Hereby, we demonstrated a much better precision of iSED with commercial control samples at both evaluated levels than in a previously published study, while patient samples yielded intrarun CVs similar to already reported data for iSED, with decreasing precision at medium and low ESR levels This is in concordance with data from a lot of validation studies of automated ESR analyzers, regardless of the analyzer type and measurement principle and can be attributed to small numbers rather than poor analytical performance, therefore not being considered clinically significant.…”

Section: Discussionsupporting

confidence: 90%

“…The comparison between iSED and the Westergren method revealed higher correlation coefficients than in a previous validation study. 13 As evidenced in this study, iSED analyzer provides accurate ESR measurement and acceptable concordance with the gold standard Westergren method, with minor discrepancies and occasionally limited agreement that can be attributed to different methodological principles. However, as ESR could be considered more a generic sign of pathophysiological alterations than a true laboratory measure, not particularly dense of specific clinical significance, for this target, iSED could provide acceptable discriminatory performance.…”

Section: Discussionmentioning

confidence: 51%

“…The comparison between iSED and the Westergren method revealed higher correlation coefficients than in a previous validation study . However, despite overall high correlations at both study sites, the disagreement and dispersion of results was frequently significant, especially at high ESR levels, meaning that a patient could have high ESR by Westergren and normal ESR measured with iSED, or vice versa, which could potentially lead to different clinical interpretation.…”

Section: Discussionmentioning

confidence: 59%

“…A volume of 100 µL of sample is aspirated directly from the ethylenediaminetetraacetic acid (EDTA) tube into a microflow cell where erythrocyte aggregation is measured using an optical detector. The rate of aggregation correlates with the sedimentation rate, and results are converted to Westergren units . A maximum of 20 samples can be analyzed at a time, with the first result being available within 3 minutes, and a continuous release of the following results every 20 seconds.…”

Section: Methodsmentioning

confidence: 99%

“…The rate of aggregation correlates with the sedimentation rate, and results are converted to Westergren units. 11,13 A maximum of 20 samples can be analyzed at a time, with the first result being available within 3 minutes, and a continuous release of the following results every 20 seconds.…”

Section: Ised Analyzermentioning

confidence: 99%

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Analytical validation of the iSED automated analyzer for erythrocyte sedimentation rate

Lapić

Miloš

Tosato

et al. 2019

Int J Lab Hematology

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Introduction iSED is an alternate automated analyzer for erythrocyte sedimentation rate (ESR) based on photometric rheology technology that estimates ESR by measuring rouleaux formation. The aim was to evaluate the analytical performance of the iSED analyzer and compare the results with the Westergren method and another alternate ESR analyzer, TEST1. Methods Validation was performed at two study sites according to the recommendations by the International Council for Standardization in Haematology and included determination of intrarun precision and inter‐run precision, bias, carryover, and method comparison, which was further assessed for samples with normal and low hematocrit, as well as per low, middle, and upper third of the analytical range. Results Intrarun coefficients of variation (CVs) with commercial controls were 4.0% and 1.8%, while inter‐run CVs 7.5% and 0.7%, for the normal and pathological range, respectively. Intrarun CVs obtained with patient samples were 19.9%, 9.9%, 10.3%, and 9.4%, the highest being for the lowest ESR value. Correlation coefficients for the comparison between iSED and Westergren were 0.862 (Site‐1) and 0.916 (Site‐2). While proportional difference with a positive bias was revealed at Site‐1, comparison at Site‐2 showed both constant and proportional difference and a negligible negative bias. Higher correlation was obtained for samples with low than normal hematocrit. Comparison between iSED and TEST1 yielded a correlation coefficient of 0.986, constant and proportional difference, and positive bias. Carryover was 3.2%. Conclusion This study proved the analytical validity of the iSED analyzer, despite minor discrepancies to the Westergren method that can be attributed to methodological differences.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 59%

Section: Methodsmentioning

confidence: 99%

Section: Ised Analyzermentioning

confidence: 99%

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Analytical validation of the iSED automated analyzer for erythrocyte sedimentation rate

Lapić

Miloš

Tosato

et al. 2019

Int J Lab Hematology

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Differences in erythrocyte sedimentation rates using a modified Westergren method and an alternate method

Schapkaitz

Raburabu

Engelbrecht

2018

Clinical Laboratory Analysis

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Determination of the erythrocyte sedimentation rate using the hematocrit‐corrected aggregation index and mean corpuscular volume

Higuchi

Watanabe

2023

Clinical Laboratory Analysis

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Background Determination of the erythrocyte sedimentation rate (ESR) by measurement of erythrocyte aggregation is an alternative to the Westergren method and can be performed rapidly. However, its principle is opaque and the ESR values obtained can deviate from Westergren method values (WG ESR) due to hematocrit. Furthermore, WG ESR is affected by particle size, but no studies have examined the effect of individual mean corpuscular volumes (MCVs). Methods Simultaneous measurement of the erythrocyte aggregation index (AI) over a 5‐s interval and determination of the complete blood count in 80 μL blood from 203 patients were performed (hematocrit, 21.4%–52.3%; MCV, 62.7–114.1 fL). ESR values were calculated with the hematocrit‐corrected AI (HAI) for comparison with WG ESR. We improved the calculation formula by using MCV. Results The sedimentation velocity of a single erythrocyte in the samples agreed well with an exponential function of HAI. ESR values calculated using HAI showed excellent correlation with WG ESR ( r = 0.899, p < 0.001; Bland–Altman analysis: bias 2.76, limits of agreement (LOA) −24.5 to 30.0), but the difference between the calculated ESR and WG ESR increased with decreasing MCV. Calculation of ESR considering both HAI and MCV eliminated the MCV‐dependent deviation and improved the correlation with WG ESR ( r = 0.920, p < 0.001, bias −2.17, LOA −24.6 to 20.3). Conclusion Calculation using HAI and MCV can rapidly provide ESR values that are highly correlated with WG ESR in clinical specimens over a wide range of hematocrit and MCV values.

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Comparison of iSED and Ves‐Matic Cube 200 Erythrocyte Sedimentation Rate Measurements With Westergren Method

Abstract: iSED sedimentation showed a poor correlation and a high bias (>10%) with the Westergren method, and Ves-Matic Cube 200 method showed a higher correlation and a lower bias than the iSED device when compared with Westergren reference method. These instruments should be carefully monitored.

Cited by 25 publications

References 8 publications

Analytical validation of the iSED automated analyzer for erythrocyte sedimentation rate

Analytical validation of the iSED automated analyzer for erythrocyte sedimentation rate

Differences in erythrocyte sedimentation rates using a modified Westergren method and an alternate method

Determination of the erythrocyte sedimentation rate using the hematocrit‐corrected aggregation index and mean corpuscular volume

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