Comparison of interleukin 18 gene expression and its serum level between Iranian colorectal cancer (CRC) patients and healthy people

Hosseini-Baraftabi, Nasibeh; Zia-Jahromi, Noosha; Talebi, Ardeshir

doi:10.2478/s11756-018-0153-z

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…After 24 hours, the cells were treated separately with different concentrations of capecitabine (75, 150, 300, and 600 µg ml − 1 ) (Cas No. 154361-50-9) and naringin (10,20,30, and 40 µg ml − 1 ) (Cas No. 10236-47-2).…”

Section: Cytotoxic Evaluation Of the Compoundsmentioning

confidence: 99%

“…The conditions of DNase treatment were as follows: 10 microliters of extracted RNA with 1 microliter of enzyme buffer and 2 microliters of DNase enzyme at 37°C for 5 minutes and then at 80°C for 5 minutes. The RNA sample (0.2 µg) was converted into cDNA according to the manufacturer's recommendations using Oligo (dT) 18 reverse transcriptase cDNA primers by Yekta Tajhiz Azma cDNA synthesis kit (Yekta Tehiz Azma, Tehran, Iran) (30).…”

Section: Investigation Of Apoptosis By Ow Cytometrymentioning

confidence: 99%

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The Combined Therapeutic Effect of Capecitabine and Naringin on HER2+ (SK-BR-3) and HER2- (MCF-7) Human Breast Cancer Cells Lines

Soltannezhad,

Jouni,

Osgoei

2023

Preprint

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Breast cancer is one of the most common cancers among women. The use of natural products to improve the effectiveness of chemotherapy drugs against the proliferation of cancer cells is increasing. Here, we hypothesized that naringin in combination with capecitabine may have a synergistic effect on inhibiting proliferation and inducing apoptosis in MCF-7 and SK-BR-3 breast cancer cell lines. MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was performed to study the effect of drugs alone and in combination on the cytotoxicity of cell lines and to determine IC50 and Combination Index (CI). Moreover, the expression of Bax and Bcl-2 and caspase3 apoptotic markers were measured by Real-Time PCR after treatment. The MTT results showed that the IC50 of naringin and capecitabine in the MCF-7 cell line was 58 µg ml− 1 and 619.36 µg ml− 1, respectively, and the IC50 of these compounds for the SK-BR-3 cell line was 56.65 µg ml− 1 and 679.51 µg ml− 1. The combined use of naringin and capecitabine led to a significant decrease in the IC50 of these compounds, and the CI values were less than 1, which indicates the synergistic effects of these compounds. The gene expression results also showed an increase in the ratio of Bax/Bcl-2 by naringin-capecitabine compared with capecitabine in both cell lines. Naringin-capecitabine-induced cell death was probably controlled by caspase-3 and Bax/Bcl-2-dependent apoptosis. Also, the combination of naringin-capecitabine has more antiproliferative properties on HER2+ cells compared with HER2−.

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Section: Cytotoxic Evaluation Of the Compoundsmentioning

confidence: 99%

Section: Investigation Of Apoptosis By Ow Cytometrymentioning

confidence: 99%