Comparison of Indirect Immunofluorescence and Passive Particle Agglutination for the Detection of Mycoplasma Pneumoniae Antibodies

Chen, Keping; Xu, Wenju; Yang, Chuankun

doi:10.7754/clin.lab.2018.180901

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“…Serological detection of M. pneumoniae is fast and more easily executed at clinics. Due to high high sensitivity and speci city, M. pneumoniae antibody detection is often used for diagnosis 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Clinical Value of Two Detection Methods for Mycoplasma Pneumoniae Antibody in the Diagnosis of Mycoplasma Pneumoniae Infection in North China

Wang

Zhu

et al. 2021

Preprint

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Background: To analyze the detection of Mycoplasma pneumoniae in adults and children in North China using two antibody detection methods, and to guide prevention and treatment.Methods: A retrospective study was conducted from September 2017 to May 2021. Mycoplasma pneumoniae total antibody was detected using particle agglutination (PA). Anti-Mycoplasma pneumoniae IgM in patients with respiratory tract infection was detected by indirect immunofluorescence. All patients were divided into 9 groups according to age: ≤1, 2-3, 4-6, 7-14, 15-18, 19-39, 40-59, 60-79, ≥80,Results: The positive rate of Mycoplasma pneumoniae total antibody in 5,666 patients with community-acquired pneumonia was 40.13%. In adults and children, the positive rates were 19.92% and 77.3% (p<0.05), respectively. The positive rates in males and females was 37.89% and 42.40% (p<0.05), respectively. The positive rate for anti-Mycoplasma pneumoniae IgM in 5,746 patients with respiratory tract infection was 28.56%, and 10.37% and 36.82% in adults and children (p<0.05), respectively. In males and females, the positive rate for anti-Mycoplasma pneumoniae IgM was 24.56% and 33.38% (p<0.05). The highest positive rates for total antibody and anti-Mycoplasma pneumoniae IgM were recorded in autumn. Of the 1,975 patients tested for Mycoplasma pneumoniae antibody using both methods simultaneously, 26.71% were negative for total antibody and 8.63% had titers ranging between 1:40 and 1:80 when positive for IgM antibody. When negative for anti-Mycoplasma pneumoniae IgM, total antibody titer was ≥1:160 in 34.94% of the patients.Conclusion: Mycoplasma pneumoniae is the main cause of respiratory tract infection and its incidence is highest in autumn. Because Mycoplasma pneumoniae was more commonly detected in women and children, screening should be strengthened in these groups.

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Section: Introductionmentioning

confidence: 99%