Comparison of in vivo targeting ability between cRGD and collagen-targeting peptide conjugated nano-carriers for atherosclerosis

Kim, Manse; Sahu, Abhishek; Kim, Jun Seok; Nam, Gi Hoon; Um, Wooram; Shin, So Jin; Jeong, Yong Yeon; Kim, In San; Kim, Kwangmeyung; Kwon, Ick Chan; Tae, Giyoong

doi:10.1016/j.jconrel.2017.11.033

Cited by 59 publications

(39 citation statements)

References 63 publications

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“…This holds true not only for clinical applications, but also for preclinical animal models like ApoE −/− mice. Thus, for a (semi) quantitative assessment of plaque components inside the aortic vessels, at the moment optical imaging is still restricted to ex vivo evaluation of fluoroprobe distribution [51][52][53][54]. In this setting, our probe is capable of delineating regions of higher intensity within the aortic arch, summing up to an overall higher signal intensity of diseased vessels versus control and also depicts the lower amount of ET A R in human atherosclerotic plaques.…”

Section: Discussionmentioning

confidence: 99%

Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis

et al. 2020

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Cardiovascular disease remains the most frequent cause of death worldwide. Atherosclerosis, an underlying cause of cardiovascular disease, is an inflammatory disorder associated with endothelial dysfunction. The endothelin system plays a crucial role in the pathogenesis of endothelial dysfunction and is involved in the development of atherosclerosis. We aimed to reveal the expression levels of the endothelin-A receptor (ETAR) in the course of atherogenesis to reveal possible time frames for targeted imaging and interventions. We used the ApoE−/− mice model and human specimens and evaluated ETAR expression by quantitative rtPCR (qPCR), histology and fluorescence molecular imaging. We found a significant upregulation of ETAR after 22 weeks of high-fat diet in the aortae of ApoE−/− mice. With regard to translation to human disease, we applied the fluorescent probe to fresh explants of human carotid and femoral artery specimens. The findings were correlated with qPCR and histology. While ETAR is upregulated during the progression of early atherosclerosis in the ApoE−/− mouse model, we found that ETAR expression is substantially reduced in advanced human atherosclerotic plaques. Moreover, those expression changes were clearly depicted by fluorescence imaging using our in-house designed ETAR-Cy 5.5 probe confirming its specificity and potential use in future studies.

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Section: Discussionmentioning

confidence: 99%

Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis

et al. 2020

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“…(G) Ex vivo imaging with GB123 in human carotid plaque (above: unstable plaque + inhibitor, middle: unstable plaque, below: stable plaque) revealed high cathepsin activity, in yellow, was found in probe-treated unstable plaques [adapted from ( 19 )]. (H) NIR fluorescence images of collected aorta from ApoE KO mice after 24 h injection of IONP-cRGD-NC, IONP-Col IV-tg-NC, IONP-NC [adapted from ( 20 )].…”

Section: Surface-enhanced Raman Spectroscopymentioning

confidence: 99%

Molecular and Nonmolecular Imaging of Macrophages in Atherosclerosis

Tang

2021

Front. Cardiovasc. Med.

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Atherosclerosis is a major cause of ischemic heart disease, and the increasing medical burden associated with atherosclerotic cardiovascular disease has become a major public health concern worldwide. Macrophages play an important role in all stages of the dynamic progress of atherosclerosis, from its initiation and lesion expansion increasing the vulnerability of plaques, to the formation of unstable plaques and clinical manifestations. Early imaging can identify patients at risk of coronary atherosclerotic disease and its complications, enabling preventive measures to be initiated. Recent advances in molecular imaging have involved the noninvasive and semi-quantitative targeted imaging of macrophages and their related molecules in vivo, which can detect atheroma earlier and more accurately than conventional imaging. Multimodal imaging integrates vascular structure, function, and molecular imaging technology to achieve multi-dimensional imaging, which can be used to comprehensively evaluate blood vessels and obtain clinical information based on anatomical structure and molecular level. At the same time, the rapid development of nonmolecular imaging technologies, such as intravascular imaging, which have the unique advantages of having intuitive accuracy and providing rich information to identify macrophage inflammation and inform targeted personalized treatment, has also been seen. In this review, we highlight recent methods and research hotspots in molecular and nonmolecular imaging of macrophages in atherosclerosis that have enormous potential for rapid clinical application.

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“…The chronic vascular inflammation and dyslipidemia exist and helps in spotting atherosclerosis. [ 121 ] The growth factors TGF‐β transform cardiomyocyte survival responses and inhibit macrophage pro‐inflammatory gene expression by promoting extracellular matrix synthesis. TGF‐β affects embryogenesis and tissue homeostasis and acts as a mediator between angiogenic and angiostatic effects.…”

Section: Mechanobiology Of Arteriosclerosis Strokes Heart Attacks mentioning

confidence: 99%

The convergence of nanotechnology‐stem cell, nanotopography‐mechanobiology, and biotic‐abiotic interfaces: Nanoscale tools for tackling the top killer, arteriosclerosis, strokes, and heart attacks

Kumar

Gulia

2020

Nano Select

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The nanotechnology‐stem cell has enabled the engineering of nanotopographical surfaces for mimicking the topological features of nano‐stem cell interfaces and stem cell niches. The nanotechnology‐stem cell, an abiotic–biotic model, the impact of the multi‐functional materials underlying support on nanomaterials interactions with stem cells plays a significant role in the development of the nanoscale tools for tracking the top killer, arteriosclerosis, strokes, and heart attacks. The biomimetic topographical surfaces, employed in cell mechanobiology (cell adhesions, migrations, and differentiation) and tissue engineering. The conformational features of the scaffold (ridges, grooves, whorls, pits, and pores, and symmetry) alter stem cell behavior and differentiation. Biomedical engineering is offering promises and explaining how the convergence of nanotechnology and stem cells has emerged as novel therapeutics. The analysis of mechanotransduction and tissue engineering is the key factor, which altogether influence the stem cell niche, can be considered for originating nanno‐stem cell interface, and a useful remedy for treating cardiovascular diseases. The Hippo pathway underlined the signaling cascade responsible for inhibiting cell proliferation. Notch signaling is a key pathway that regulates the modulation of cardiomyocyte survival, cardiac stem cell differentiation. The Wnt/β‐catenin signaling involved in chronic infusion of angiotensin II (Ang II), cardiomyocytes, and cardiac fibroblasts.

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Comparison of in vivo targeting ability between cRGD and collagen-targeting peptide conjugated nano-carriers for atherosclerosis

Cited by 59 publications

References 63 publications

Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis

Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis

Molecular and Nonmolecular Imaging of Macrophages in Atherosclerosis

The convergence of nanotechnology‐stem cell, nanotopography‐mechanobiology, and biotic‐abiotic interfaces: Nanoscale tools for tackling the top killer, arteriosclerosis, strokes, and heart attacks

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