Comparison of in vivo and in vitro methodologies for the assessment of arsenic bioavailability in contaminated soils

Juhasz, Albert L.; Smith, Euan; Weber, John; Rees, Matthew; Rofe, Allan M.; Kuchel, Tim; Sansom, Lloyd; Naidu, Ravi

doi:10.1016/j.chemosphere.2007.05.018

Cited by 145 publications

(146 citation statements)

References 19 publications

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“…There are a number of uncertainties in relating a bioaccessible value to the amount of PHE absorbed by a human subject. If we assume the swine gastrointestinal model is a good surrogate for humans (Miller and Ullrey, 1987;Moughan et al, 1992) and that there is a linear relationship between bioaccessibility and bioavailability (Schroder et al, 2003;Schroder et al, 2004;Drexler and Brattin, 2007;Juhasz et al, 2007a) then the data from this and similar studies can be used to provide information on the reliability of the data provided to the risk assessor. A simulation was set up using relative bioavailability data on a set of theoretical soils covering the range of 1 to 90% (1, 5, 25, 50, 75 and 90%) with theoretical relative bioaccessibility data on the same soil samples.…”

Section: Discussionmentioning

confidence: 99%

“…Bioavailability data from actual human soil feeding tests is scarce (Maddaloni et al, 1998;Stanek et al,2010) and although in vivo animal studies are have been carried out, these are, in general costly, time consuming, have ethical constraints and there is usually only a limited amount of soil available (Freeman et al, 1992;Freeman et al, 1993;Ruby et al, 1993;Freeman et al, 1995;Golub et al, 1999;Rodriguez et al, 1999;Ellickson et al, 2001;Schroder et al, 2003;Schroder et al, 2004;Marschner et al, 2006;Makris et al, 2008;Bannon et al, 2009;Nagar et al, 2009) and Juhasz et al (2007aJuhasz et al ( , 2008Juhasz et al ( , 2009aJuhasz et al ( , 2009b). An alternative is the application of in vitro models that simulate the GI tract.…”

Section: Introductionmentioning

confidence: 99%

“…The key contaminants included in this work are arsenic (As), lead (Pb) and cadmium (Cd) as they are potentially harmful to human health (ATSDR, 2007b;ATSDR, 2007a;ATSDR, 2008) and the most common elements undergoing bioaccessibility research Ruby et al, 1993;Ruby et al, 1996;Albores et al, 2000;Oomen et al, 2002;Marschner et al, 2006;Chan et al, 2007;Datta et al, 2007;Drexler and Brattin, 2007;Finzgar et al, 2007;Hansen et al, 2007;Juhasz et al, 2007aJuhasz et al, , 2007bLjung et al, 2007;Sarkar et al, 2007;Subacz et al, 2007;Turner and Ip, 2007;Van de Wiele et al, 2007;Beak et al, 2008;Moseley et al, 2008;Girouard and Zagury, 2009;Morman et al, 2009;Nagar et al, 2009;Poggio et al, 2009;Smith et al, 2009;Zhang et al, 2009;Demetriades et al, 2010;Juhasz et al2010). …”

Section: Introductionmentioning

confidence: 99%

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An inter-laboratory trial of the unified BARGE bioaccessibility method for arsenic, cadmium and lead in soil

Wragg

Cave

Basta

et al. 2011

Science of The Total Environment

166

202

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The Bioaccessibility Research Group of Europe (BARGE) has carried out an interlaboratory trial of a proposed harmonised in vitro physiologically based ingestion bioaccessibility procedure for soils, called the Unified BARGE Method (UBM). The UBM includes an initial saliva phase and simulated stomach and intestine compartments. The trial involved the participation of seven laboratories (five European and two North American) providing bioaccessibility data for As (11 samples), Cd (9 samples) and Pb (13 samples) using soils with in vivo relative bioavailability data measured using a swine model. The results of the study were compared with benchmark criteria for assessing the suitability of the UBM to provide data for human health risk assessments. Mine waste and slag soils containing high concentrations of As caused problems of poor repeatability and reproducibility which were alleviated when the samples were run at lower soil to solution ratios. The study showed that the UBM met the benchmark criteria for both the stomach and stomach & intestine phase for As. For Cd, three out of four criteria were met for the stomach phase but only one for the stomach & intestine phase. For Pb two, out of four criteria were met for the stomach phase and none for the stomach & intestine phase. However, the study recommends tighter control of pH in the stomach phase extraction to improve between-laboratory variability, more reproducible in vivo validation data and that a follow up inter-laboratory trial should be carried out.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An inter-laboratory trial of the unified BARGE bioaccessibility method for arsenic, cadmium and lead in soil

Wragg

Cave

Basta

et al. 2011

Science of The Total Environment

166

202

View full text Add to dashboard Cite

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“…The measurement of the bioavailability of As in soil requires in vivo testing using humans or animal surrogates, a time consuming, costly and ethically challenging process. However, in vitro bioaccessibility testing has been developed and validated specifically to provide a conservative estimate of bioavailability (Basta et al, 2007;Caboche, 2009;Denys et al, 2012;Juhasz et al, 2007a). The in vitro bioaccessibility assay estimates the fraction of As released from the soil into solution in the gastro-intestinal (GI) tract in a form that can potentially be absorbed into the blood stream (Paustenbach, 2000;Wragg and Cave, 2003;Intawongse and Dean, 2006).…”

Section: Introductionmentioning

confidence: 99%

Anthropogenic and geogenic impacts on arsenic bioaccessibility in UK topsoils

Appleton¹,

Cave²,

Wragg³

2012

Science of The Total Environment

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Predictive linear regression (LR) modelling between bioaccessible arsenic (B-As) and a range of total elemental compositions and soil properties was executed in order to assess the potential for developing a national B-As dataset for the UK. LR indicates that total arsenic (As) is the only highly significant independent variable for estimating B-As in urban areas where it explains 75-92% of the variance. The broad compatibility of the London, Glasgow and Swansea regression models suggests that application of these models to estimate bioaccessible As in UK soils impacted by diffuse anthropogenic urban contamination and non-ferrous metal processing should be relatively accurate. In areas dominated by Jurassic ironstones and associated clays and limestones, total As, P and pH are significant, accounting for 53, 14 and 5%, respectively, of the B-As variance. Models based on total As as the sole predictor in the combined Jurassic and Cretaceous sedimentary ironstones datasets explain about 40% of the B-As variance. The median As bioaccessible fraction (%As-BAF) is 19 to 28% in the anthropogenic contamination impacted urban domains, but much lower (5-9%) in geogenic terrains dominated by ironstones. Results of this study can be used as part of a lines of evidence approach to localised risk assessment but should not be used to replace bioaccessibility testing at individual sites where local conditions may vary considerably from the broad overview presented in this study.

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“…A simple bioaccessibility extraction test (SBET) (Drexler and Brattin 2007;Juhasz et al 2007;Kim et al 2002;Madrid et al 2008) was used to assess the bioaccessibility of As, Cu, Pb, and Zn. Briefly, 50 mL of the 0.4 M glycine extraction solution was added to 0.5 g of sieved (\250 lm fraction) air-dried soil material in 100 mL polythene centrifuge tubes.…”

Section: Total Concentration and Bioaccessibility Of Heavy Metalsmentioning

confidence: 99%

Bioaccessibility of heavy metals in soils cannot be predicted by a single model in two adjacent areas

Zhu

Yang

Wei

et al. 2015

Environ Geochem Health

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The objective of this study was to examine whether a single model could be used to predict the bioaccessibility of heavy metals in soils in two adjacent areas and to determine the feasibility of using existing data sets of total metal concentrations and soil property parameters (e.g., pH, total organic carbon, and soil texture) when predicting heavy metal bioaccessibility. A total of 103 topsoil samples were collected from two adjacent areas (Baotou and Bayan Obo). A total of 76 samples were collected from Baotou, and 27 were collected from Bayan Obo. The total and bioaccessible concentrations of arsenic (As), copper (Cu), lead (Pb), and zinc (Zn) were measured following complete composite acid digestion and a simple bioaccessibility extraction test. The average total concentrations of As, Cu, Pb, and Zn were 8. 95, 27.53, 28.40, and 79.50 mg/kg, respectively, in Baotou and 18.12, 30.75, 38.09, and 87.62 mg/kg in Bayan Obo. Except for As, these values were similar in both areas. The average bioaccessible heavy metal concentrations (Bio-HMs) for each target HM were also similar. In Baotou, the average Bio-HM values for As, Cu, Pb, and Zn were 1.16, 3.76, 16.31, and 16.10 mg/kg, respectively, and 1.26, 2.51, 14.31, and 8.68 mg/kg in Bayan Obo. However, the relative bioaccessibilities for each HM in Baotou were greater than those in Bayan Obo, with mean values for Pb, Zn, Cu, and As of 57, 20, 17, and 12 %, respectively, in Baotou and 40, 11, 9, and 8 % in Bayan Obo. In both areas, prediction models were successfully created using heavy metal concentrations and soil physicochemical parameters; however, models of the same target element differed between the areas, which indicated that a common model for both sites does not exist. Bio-HMs were highly affected by soil properties, which were found to differ between the adjacent areas. In addition, soil properties with large variations played major roles in the predictive models. This study highlights the importance of incorporating physical and chemical parameters that vary greatly when building predictive models of heavy metal bioaccessibility in soil. A similarity in soil properties between areas might be a prerequisite for the creation of a common predictive model for soil Bio-HMs.

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Comparison of in vivo and in vitro methodologies for the assessment of arsenic bioavailability in contaminated soils

Cited by 145 publications

References 19 publications

An inter-laboratory trial of the unified BARGE bioaccessibility method for arsenic, cadmium and lead in soil

An inter-laboratory trial of the unified BARGE bioaccessibility method for arsenic, cadmium and lead in soil

Anthropogenic and geogenic impacts on arsenic bioaccessibility in UK topsoils

Bioaccessibility of heavy metals in soils cannot be predicted by a single model in two adjacent areas

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