Comparison of In Vitro Assays of Cellular Toxicity in the Human Hepatic Cell Line HepG2

The Mechanism of Membrane Disruption by Cytotoxic Amyloid Oligomers Formed by Prion Protein(106–126) Is Dependent on Bilayer Composition

“…1, PrP(106 -126) oligomers caused a significant increase in the death of N2a subclones 1 and 2, PC-12, and SH-SY5Y cells, as monitored using the Toxilight assay after 24 h of incubation. This colorimetric assay uses the release of adenylate kinase from cells as an indicator of cell damage or death (42). In all cases, 100 M PrP(106 -126) oligomers exhibited a level of cytotoxicity similar to 5 M staurosporine, which is known to initiate apoptosis in a wide range of cell types (43).…”

Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

“…Over the past 50 years, the AB assay has been widely used in studies of cell viability and cytotoxicity for biological and environmental applications (Rampersad, 2012;Vega-Avila and Pugsley, 2011;White et al, 1996). The bioassay can also be used to establish the relative cytotoxicity of agents within various chemical classes (Bopp and Lettieri, 2008;Borra et al, 2009;Mikus and Steverding, 2000;Miret et al, 2006). Using the REDOX indicator resazurin (oxidised form), it is possible to spectrophotometrically measure the cellular proliferation.…”

Cytotoxic Effects of Activated Bromine on Human Fetal Osteoblasts In Vitro

“…Cellular cytotoxicity can be evaluated by studying different parameters (18,19). These include measurement of mitochondrial activity, cellular adenosine triphosphate, cellular necrosis, and fluorometric assay.…”