Comparison of immunoreactive renin and plasma renin activity in human plasma.

Abstract

Measurement of immunoreactive plasma renin concentration (PRC) using direct radioimmunoassay (RIA) was compared with the common procedure, measurement of plasma renin activity (PRA). The sensitivity of the PRC assay was 5 pg/ml. In 67 normal subjects aged 45.2+1.2 year, the mean PRC value was 17.0+0.9 pg/ml in the recumbent position and 38.0 + 5.4 pg/ml in the upright position. In patients with high renin essential hypertension and renovascular hypertension, discrepancies were observed between changes in PRA and PRC at 60 min after the administration of captopril. In a patient with Bartter's syndrome PRC was markedly elevated (393 pg/ml) and the chages in PRA and in PRC after captopril were very different (452% vs. 1249%). In all 10 cases of primary hyperaldosteronism PRC was less than 5 pg/ml. The correlation coefficient between PRC and PRA was 0.85 (n =227, p <0.01). The slope of the regression line between PRA and PRC decreased in proportion to PRC values. Direct RIA for PRC is likely to be useful for the determination of plasma active renin when renin levels are high or substrate concentrations are abnormal. Moreover, the combined use of PRA and PRC measurements might be useful in assessing abnormalities in renin substrate concentration as well as in PRO. active renin ; PRA ; monoclonal antibodiesThe plasma renin level is an important indicator of the state of reninangiotensin (R-A) system. The development of methods for measurement of plasma renin level has contributed greatly to our understanding of both clinical and pathophysiological aspects of the R-A system. Recently, Corvol and col-

Comparative analysis of direct renin levels and plasma renin activity for the purpose of monitoring the patients with congenital adrenal cortical hyperplasia

“…Это под-тверждается: 1) почти равной степенью отклонения сравниваемых показателей от нормы, 2) отсутствием значимых различий показателей при сравнении меж-ду собой подгрупп с отклонением в бо льшую и мень-шую сторону и 3) наличием сильной статистически значимой положительной корреляции между показа-телями. Как и в работе К. Tsunoda [2], несмотря на сильную корреляцию прямого ренина и АРП в обеих группах, выявлена тенденция к снижению коэффи-циента корреляции между этими показателями при возрастании концентрации прямого ренина. На ос-новании коэффициента корреляции между 17-ОНП и двумя исследуемыми показателями нами сделан вывод об отсутствии значимой их зависимости от компенсации глюкокортикоидного и минералокор-тикоидного дефицита, что было показано и ранее [8].…”

Direct Measurement of Immunoreactive Renin during Changes of Posture in Man

“…France), a technique previously described [4], Intra-assay variance was 9.8% (54.8 ± 1.2 ng/l. n = 20) and interassay variance 9.6% (36.0 ± 1.2 ng/1.…”

“…3]. The applicability of this new direct RIA as compared to the conventional method of measuring PRA has been studied in different forms of hypertension [4] and in aging [5], However, there are no reports of studies on changes in immunoreactive renin during a postural test. This test is commonly used to examine renin and aldosterone reactivity in humans [6.…”