Comparison of immunologic and molecular assays for the diagnosis of gastrointestinal viral infections

Rovida, Francesca; Campanini, Giulia; Sarasini, Antonella; Adzasehoun, Kodjo Messan Guy; Piralla, Antonio; Baldanti, Fausto

doi:10.1016/j.diagmicrobio.2012.09.016

Cited by 32 publications

(26 citation statements)

References 11 publications

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“…Nevertheless, these tools have limited sensitivity . In recent years, real-time PCR techniques have shown greater sensitivity and specificity than immunological assays for detection of gastrointestinal viruses (Clark and McKendrick, 2004;Rovida et al 2013;Van Maarseveen et al 2010;Wilhelmi et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of gastrointestinal viral infections in hospitalized patients

Rovida

Campanini

Piralla

et al. 2013

Diagnostic Microbiology and Infectious Disease

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Gastrointestinal viral syndromes are a common cause of morbidity and mortality in humans worldwide. Etiological agents include a large number of viruses encompassing several orders, families, and genera. During the period April 2011 to April 2012, 689 stool samples from as many patients hospitalized at the Fondazione IRCCS Policlinico San Matteo of Pavia exhibiting gastrointestinal syndromes were examined for the presence of rotavirus, norovirus, astrovirus, adenovirus, rhinovirus, enterovirus, parechovirus, bocavirus, coronavirus, sapovirus, cosavirus, and aichi virus using polymerase chain reaction assays. Gastrointestinal viral agents were detected in 246 (36%) patients of the 689 analyzed. Adenovirus and norovirus were the most common viruses in this cohort, while aichi virus was the only gastrointestinal agent not detected. Surprisingly, rhinovirus was one of the most frequently detected viruses. However, a potential association with gastroenteritis remains to be confirmed.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of gastrointestinal viral infections in hospitalized patients

Rovida

Campanini

Piralla

et al. 2013

Diagnostic Microbiology and Infectious Disease

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“…For virus detection, a panel of real-time RT-PCR or PCR detecting norovirus, astrovirus, rotavirus, adenovirus and sapovirus was performed as previously reported [20, 21]. …”

Section: Methodsmentioning

confidence: 99%

FilmArray™ GI panel performance for the diagnosis of acute gastroenteritis or hemorragic diarrhea

et al. 2017

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BackgroundAcute gastroenteritis is a common cause of morbidity and mortality in humans worldwide. The rapid and specific identification of infectious agents is crucial for correct patient management. However, diagnosis of acute gastroenteritis is usually performed with diagnostic panels that include only a few pathogens. In the present bicentric study, the diagnostic value of FilmArray™ GI panels was assessed in unformed stool samples of patients with acute gastroenteritis and in a series of samples collected from pediatric patients with heamorragic diarrhea. The clinical performance of the FilmArray™ gastrointestinal (GI) panel was assessed in 168 stool samples collected from patients with either acute gastroenteritis or hemorragic diarrhea. Samples showing discordant results between FilmArray and routine methods were further analyzed with an additional assay.ResultsOverall, the FilmArray™ GI panel detected at least one potential pathogen in 92/168 (54.8%) specimens. In 66/92 (71.8%) samples, only one pathogen was detected, while in 26/92 (28.2%) multiple pathogens were detected.The most frequent pathogens were rotavirus 13.9% (22/168), Campylobacter 10.7% (18/168), Clostridium difficile 9.5% (16/168), and norovirus 8.9% (15/168). Clostridium difficile was identified only in patients with acute gastroenteritis (p < 0.01), while STEC was detected exclusively in patients with hemorragic diarrhea (p < 0.01). In addition, Campylobacter spp., Salmonella spp., EPEC and E. coli producing Shiga-like toxin were more frequently detected in patients with hemorragic diarrhea (p < 0.05). The overall percent agreement calculated in samples was 73.8% and 65.5%, while 34.5% were discordant. After additional confirmatory analyses, the proportion of discordant samples decreased to 7.7%. Rotavirus and astrovirus were the most frequently unconfirmed pathogens.ConclusionIn conclusion, the FilmArray™ GI panel has proved to be a valuable new diagnostic tool for improving the diagnostic efficiency of GI pathogens.

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“…Although the assays do not enable typing of the isolates, they are useful for rapid detection of the virus when large numbers of samples must be analyzed (167,(276)(277)(278)(279). EIA-based techniques have been reported to have the same sensitivity as direct EM observation (228) but obviously are much less time-consuming.…”

Section: Laboratory Diagnostics Early Developments: Electron Microscomentioning

confidence: 99%

Human Astroviruses

2014

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SUMMARY Human astroviruses (HAtVs) are positive-sense single-stranded RNA viruses that were discovered in 1975. Astroviruses infecting other species, particularly mammalian and avian, were identified and classified into the genera Mamastrovirus and Avastrovirus . Through next-generation sequencing, many new astroviruses infecting different species, including humans, have been described, and the Astroviridae family shows a high diversity and zoonotic potential. Three divergent groups of HAstVs are recognized: the classic ( MAstV 1 ), HAstV-MLB ( MAstV 6 ), and HAstV-VA/HMO ( MAstV 8 and MAstV 9 ) groups. Classic HAstVs contain 8 serotypes and account for 2 to 9% of all acute nonbacterial gastroenteritis in children worldwide. Infections are usually self-limiting but can also spread systemically and cause severe infections in immunocompromised patients. The other groups have also been identified in children with gastroenteritis, but extraintestinal pathologies have been suggested for them as well. Classic HAstVs may be grown in cells, allowing the study of their cell cycle, which is similar to that of caliciviruses. The continuous emergence of new astroviruses with a potential zoonotic transmission highlights the need to gain insights on their biology in order to prevent future health threats. This review focuses on the basic virology, pathogenesis, host response, epidemiology, diagnostic assays, and prevention strategies for HAstVs.

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Comparison of immunologic and molecular assays for the diagnosis of gastrointestinal viral infections

Cited by 32 publications

References 11 publications

Molecular detection of gastrointestinal viral infections in hospitalized patients

Molecular detection of gastrointestinal viral infections in hospitalized patients

FilmArray™ GI panel performance for the diagnosis of acute gastroenteritis or hemorragic diarrhea

Human Astroviruses

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