Comparison of immunodulatory properties of dental pulp stem cells derived from healthy and inflamed teeth

Yazid, Farinawati; Gnanasegaran, Nareshwaran; Kunasekaran, Wijenthiran; Govindasamy, Vijayendran; Musa, Sabri

doi:10.1007/s00784-014-1207-4

Cited by 57 publications

(62 citation statements)

References 35 publications

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“…In this study, our results were concurrent with previous reports whereby cells were of fibroblastic phenotype and could be expanded in culture flasks (Yazid et al 2014;Abu Kasim et al 2012;Govindasamy et al 2010a). On top of that SHED were shown to have decent outcome in terms of cell count, PDT as well as senescence assay.…”

Section: Discussionsupporting

confidence: 92%

“…Three-lineage differentiation Mulitipotency of SHED was assessed by differentiating them into adipocytes, chondrocytes, and osteocytes as described previously (Yazid et al 2014;Govindasamy et al 2011). Briefly, adipogenic differentiation was initiated by inducing the cells with 200 mM indomethacin, 0.5 mM 3-isobutyl-1-methyxanthine (IMBX), 10 mg/mL insulin and 1 mM dexamethasone (all reagents from Sigma-Aldrich).…”

Section: Growth Kineticsmentioning

confidence: 99%

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ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

Gnanasegaran

Govindasamy²,

Musa

et al. 2014

Cytotechnology

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Among the debilitating diseases, neurological related diseases are the most challenging ones to be treated using cell replacement therapies. Recently, dental pulp stem cells (SHED) were found to be most suitable cell choice for neurological related diseases as evidenced with many preclinical studies. To enhance the neurological potential of SHED, we recapitulated one of the pharmacological therapeutic tools in cell replacement treatment, we pre-conditioned dental pulp stem cells (SHED) with culture medium of ReNCell VM, an immortalized neuron progenitor cell, prior to neurogenesis induction and investigated whether this practice enhances their neurogenesis potential especially towards dopaminergic neurons. We hypothesed that the integration of pharmacological practices such as co-administration of various drugs, a wide range of doses and duration as well as pre-conditioning into cell replacement may enhance the efficacy of stem cell therapy. In particular, pre-conditioning is shown to be involved in the protective effect from some membrano-tropic drugs, thereby improving the resistance of cell structures and homing capabilities. We found that cells pre-treated with ReNCell VM conditioned medium displayed bipolar structures with extensive branches resembling putative dopaminergic neurons as compared to nontreated cells. Furthermore, many neuronal related markers such as NES, NR4A2, MSI1, and TH were highly expressed (fold changes [ 2; p \ 0.05) in pretreated cells. Similar observations were detected at the protein level. The results demonstrate for the first time that SHED pre-conditioning enhances neurological potential and we suggest that cells should be primed to their respective environment prior to transplantation.

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Section: Discussionsupporting

confidence: 92%

Section: Growth Kineticsmentioning

confidence: 99%

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

Gnanasegaran

Govindasamy²,

Musa

et al. 2014

Cytotechnology

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“…Yazid et al reported that MSCs derived from inflamed pulp deciduous tissues were highly dysfunctional in MSC characteristics, stemness, and immunomodulatory properties [17]. In contrast, our results showed that there were no significant differences between SCIDs and SHEDs in their in vitro proliferation and multidifferentiation potentials.…”

Section: Discussioncontrasting

confidence: 79%

Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth

Diao

Wang

et al. 2014

BioMed Research International

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Stem cells isolated from exfoliated deciduous teeth (SHEDs) are highly capable of proliferation and differentiation, and they represent good cell sources for mesenchymal stem cell- (MSC-) mediated dental tissue regeneration, but the supply of SHEDs is limited. A previous study found that stem cells could be isolated from inflamed tissues, but it is unknown whether primary dental pulp diagnosed with irreversible pulpitis might contain stem cells with appropriate tissue regeneration capacity. In this study, we aimed to isolate stem cells from both inflamed pulps of deciduous teeth (SCIDs) and SHEDs from Chinese children and to compare their proliferation and differentiation potentials. Our results showed that SCIDs were positive for cell surface markers, including CD105, CD90, and CD146, and they had high proliferation ability and osteogenic, adipogenic, and chondrogenic differentiation potentials. There was no significant difference in proliferation and differentiation potentials between SCIDs and SHEDs. The mRNA of inflammatory factors, including IL-1β, IL-6, and TNF-α, was expressed at similar levels in SCIDs and SHEDs, but SCIDs secreted more TNF-α protein. In conclusion, our in vitro results showed that SCIDs have proliferation and differentiation potentials similar to those of SHEDs. Thus, SCIDs represent a new potentially applicable source for MSC mediated tissue regeneration.

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“…Key and well-characterized cytokines, including IL-1a, IL-1b, TNF-a, IL-4, IL-6, IL-8, and IL-10, are highly expressed by structural cells from pulp tissue, such as odontoblasts, fibroblasts and mesenchymal stem cells, along with immune cells, when they are exposed to bacterial and dental materials components. [24][25][26][27][28] These molecules play regulatory functions for lymphocytes, macrophages, and neutrophils, activating the inflammatory cascade, 29,30 which may result in release of proteolytic enzymes, such as metalloproteinases, with the consequent disruption of extracellular matrix components. 31,32 Prostaglandin E 2 , synthesized by COX-2 gene is also a major pro-inflammatory mediator, which is known to accelerate inflammation and injury in several tissues, including the dental pulp.…”

Section: Discussionmentioning

confidence: 99%

Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel

Soares

Basso

Scheffel

et al. 2015

Archives of Oral Biology

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Comparison of immunodulatory properties of dental pulp stem cells derived from healthy and inflamed teeth

Abstract: SCDIP is not a viable therapeutic cell source especially when used in graft versus host disease (GvHD) and organ rejection.

Cited by 57 publications

References 35 publications

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth

Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel

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