Comparison of Immunodot and Western Blot Assays for Diagnosing Lyme Borreliosis

Fawcett, Paul T.; Rosé, Carlos D.; Gibney, Kathleen M.; Doughty, Robert A.

doi:10.1128/cdli.5.4.503-506.1998

Cited by 12 publications

(9 citation statements)

References 9 publications

(11 reference statements)

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“…Cross et.al. described the case of a pre-pubescent female who developed PANS with a positive Cunningham Panel, was serologically positive for Streptococcus but also for several tick-borne infections including Bbsl, B. henselae, and Babesia recombinant proteins as test antigens, is more sensitive and specific than the Lyme ELISA and the Lyme Western Blot [54][55][56]. While cross-reactivity of some Borrelia proteins with antigens from other bacteria and viruses is well known [40], the presence of IgG antibodies at 23-kdA (outer surface protein [Osp]C), 34-kdA (OspB) and at 39-kdA are considered specific and therefore diagnostic for B. burgdorferi [57][58][59][60].…”

Section: Discussionmentioning

confidence: 99%

Microbial Induced Autoimmune Inflammation as a Cause of Mental Illness in Adolescents: A Case Series

Kinderlehrer¹,

Brown²

2021

GJMR

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The incidence of mental health disorders in adolescents continues to rise. The cause of the increase in mental illness is multifactorial, including both environmental and biological causes. To investigate the latter, ten adolescents at a psychiatric residential treatment center in Colorado with the DSM-5 diagnosis of major depressive disorder (MDD), of whom seven were additionally diagnosed with generalized anxiety disorder (GAD), were chosen at random for further serologic study. Testing revealed exposureto group A Streptococcus(GAS) in 2 of 10 (20%); Borrelia Burgdorferi sensulato (Bbsl)in 2 of 10 (20%); and Bartonella speciesin 3 of 10 (30%). In addition, 9 of 10 (90%) subjects had abnormal Cunningham Panels, which measures levels of antineuronal antibodies that have been associated with psychiatric disturbances. Given the degree of psychological dysfunction in these adolescents requiring intensive residential treatment, this case series lends support to the hypothesis that exposure to infectious agents may play a role, perhaps by autoimmune mechanisms, in the significant and ongoing rise in the rate of neuropsychiatric illness in adolescents. This preliminary report adds to this premise and requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Microbial Induced Autoimmune Inflammation as a Cause of Mental Illness in Adolescents: A Case Series

Kinderlehrer¹,

Brown²

2021

GJMR

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“…Since dot blot has almost the same reaction scheme as western blot, except for the protein separation step (SDS-PAGE), this method is frequently chosen as a replacement for western blot. Some routine dot blots have been developed to replace western blot and to standardize dot blot for large sample sizes [6,8]. However, to the best of our knowledge, there is no report about signal normalization of dot blot results.…”

Section: Discussionmentioning

confidence: 99%

“…CV values below 10% are known to be a characteristic of a good method [26]. Conventional dot blot method belongs to a yes/no assay type [8,27]. Častorálová et al used dot blot for quantification of protein expression, although this was done without signal normalization to a housekeeping protein [12].…”

Section: Discussionmentioning

confidence: 99%

“…Dot blot has similar technical features and specificity as western blot, but is the more sensitive method [6,7]. Currently dot blot is used in routine sample analysis [8][9][10][11], and at the same time it has been proven to work well with proteins over a wide range of molecular weights [12]. Most of the dot blot methods which have been developed use only one developing signal [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Dealing with Large Sample Sizes: Comparison of a New One Spot Dot Blot Method to Western Blot

Putra¹,

Tsuprykov²,

Websky³

et al. 2014

Clin. Lab.

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Background: Western blot is the gold standard method to determine individual protein expression levels. However, western blot is technically difficult to perform in large sample sizes because it is a time consuming and labor intensive process. Dot blot is often used instead when dealing with large sample sizes, but the main disadvantage of the existing dot blot techniques, is the absence of signal normalization to a housekeeping protein. Methods: In this study we established a one dot two development signals (ODTDS) dot blot method employing two different signal development systems. The first signal from the protein of interest was detected by horseradish peroxidase (HRP). The second signal, detecting the housekeeping protein, was obtained by using alkaline phosphatase (AP). Results: Inter-assay results variations within ODTDS dot blot and western blot and intra-assay variations between both methods were low (1.04-5.71%) as assessed by coefficient of variation. Conclusions: ODTDS dot blot technique can be used instead of western blot when dealing with large sample sizes without a reduction in results accuracy.

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“…More defined immunoblots based on recombinant proteins have been evaluated (12,14,16,19,21,32). However, some technical problems are associated with these assays, and not all diagnostically important bands are available as recombinant proteins.…”

Section: Discussionmentioning

confidence: 99%

A European Multicenter Study of Immunoblotting in Serodiagnosis of Lyme Borreliosis

Robertson

Guy

Andrews³

et al. 2000

J Clin Microbiol

144

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European multicenter study of immunoblotting for the serodiagnosis of Lyme borreliosis showed considerable variation in results obtained from tests with a panel of 227 serum samples. Six laboratories used different immunoblot methods, and a wide range of bands was detected in all the assays. Multivariable logistic regression analysis of data from individual laboratories was used to determine the most discriminatory bands for reliable detection of antibodies to Borrelia burgdorferi sensu lato. These bands were used to construct individual interpretation rules for the immunoblots used in the six laboratories. Further analysis identified a subset of eight bands, which were important in all the laboratories, although with variations in significance. Possible European rules, all closely related, were formulated from these bands, although there was no single rule that gave high levels of sensitivity and specificity for all the laboratories. This is a reflection of the wide range of methodologies used, especially the use of different species and strains of B. burgdorferi sensu lato. The panel of European rules provides a framework for immunoblot interpretation which may be adapted in relation to the characteristics of Lyme borreliosis in local areas.

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Comparison of Immunodot and Western Blot Assays for Diagnosing Lyme Borreliosis

Cited by 12 publications

References 9 publications

Microbial Induced Autoimmune Inflammation as a Cause of Mental Illness in Adolescents: A Case Series

Microbial Induced Autoimmune Inflammation as a Cause of Mental Illness in Adolescents: A Case Series

Dealing with Large Sample Sizes: Comparison of a New One Spot Dot Blot Method to Western Blot

A European Multicenter Study of Immunoblotting in Serodiagnosis of Lyme Borreliosis

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