Comparison of immunocytochemistry, reverse transcriptase polymerase chain reaction, and nucleic acid sequence–based amplification for the detection of circulating breast cancer cells

Lambrechts, A. C.; Bosma, Astrid; Klaver, Siegina G.; Top, B.; Perebolte, L; Veer, Laura J. van ’t; Rodenhuis, S.

doi:10.1023/a:1006261731125

Cited by 64 publications

(45 citation statements)

References 33 publications

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“…Based on experiences it is unlikely that false positive results can be avoided if only a single marker gene is used in a RT -PCR based test system (Lambrechts et al, 1999). Using more than a single marker gene, as applied in our experiments, is a potential way to overcome the problem of illegitimate expression (Chelly et al, 1989), assuming that there is a little chance of encountering significant illegitimate mRNA of more than one gene at a time.…”

Section: Discussionmentioning

confidence: 96%

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Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple mRNA markers

et al. 2004

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Disseminated breast tumour cells in sentinel lymph nodes (SNs) were evaluated by quantitative real-time PCR and the sensitivity of this assay was compared to the routine histological analysis. First, several candidate marker genes were tested for their specificity in axillary lymph nodes (ALN) of 50 breast cancer patients and 43 women without breast cancer. The marker gene panel selected, designed to detect the mRNA of CK19, p1B, EGP2 and SBEM, was subsequently applied to detect metastases in 70 SNs that were free of metastases as determined by standard histological evaluation. Remarkably, seven negative SNs showed increased marker gene expression, suggesting the presence of (micro) metastases. Four of these seven SNs positive by real-time PCR proved to contain tumour deposits after careful review of the slides or further sectioning of the paraffin-embedded material. In three PCR positive SNs, however, no tumour cells were found by haematoxylin and eosin staining (H&E) and immunohistologically analysis. The quantitative real-time PCR assay with multiple mRNA markers for the detection of disseminated breast cancer cells in SNs thus resulted in an upstaging of SNs containing metastastic disease of 10% compared to the routine histological analysis. The application of this technique may be of clinical relevance, as it is suggested that micrometastatic disease in SNs are associated with further nodal non-SN metastases in breast cancer.

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Section: Discussionmentioning

confidence: 96%

“…In total, 1 mg total RNA was used for cDNA synthesis (20 ml), as described previously (Lambrechts et al, 1999).…”

Section: Rna Isolation and Quantitative Real-time Pcrmentioning

confidence: 99%

Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple mRNA markers

et al. 2004

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“…Additionally, a recent RT‐PCR system has the ability to assess multiple gene expressions for the detection of CTC in one run. However, several investigators identified some limitations in the clinical application of RT‐PCR assays to the detection of CTC 15, 16. False‐positive results associated with RT‐PCR may be yielded as a result of the illegitimate expression of targeted genes by normal cells and epidermal contamination in blood collecting or processing 15.…”

Section: Detection Of Ctcmentioning

confidence: 99%

“…However, several investigators identified some limitations in the clinical application of RT‐PCR assays to the detection of CTC 15, 16. False‐positive results associated with RT‐PCR may be yielded as a result of the illegitimate expression of targeted genes by normal cells and epidermal contamination in blood collecting or processing 15. Furthermore, false‐negative results may be obtained as a result of the heterogeneous expression of the targeted markers 16.…”

Section: Detection Of Ctcmentioning

confidence: 99%

Clinical significance of circulating tumor cells in blood from patients with gastric cancer

Arigami

Uenosono

Yanagita

et al. 2017

Annals of Gastroent Surgery

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Circulating tumor cells (CTC) have been focused on as a target for detecting occult tumors, predicting therapeutic responses and prognoses, and monitoring postoperative recurrence in the clinical management of patients with various malignancies, including gastric cancer. Recent advances in molecular diagnostic tools have contributed to high sensitivity and specificity for the detection of CTC. A conspicuous disparity exists in the incidence of CTC among studies. However, a close relationship has been reported between positivity for CTC and well‐known prognostic clinicopathological factors including depth of tumor invasion, lymph node metastasis, stage, and lymphatic and venous invasion in patients with gastric cancer. According to most studies published on the clinical impact of CTC, the presence of CTC negatively affects the prognosis of patients with gastric cancer. Moreover, the study of CTC based on a meta‐analysis demonstrated their importance as a poor prognostic indicator. In clinical management, pre‐ and post‐therapeutic monitoring of CTC using liquid biopsy may be useful for early detection of subclinical patients or disease recurrence, prediction of tumor progression, and administrative control of adjuvant chemotherapy. Although their functional properties remain unclear, molecular profiling of CTC may contribute to the development of personalized treatment that effectively inhibits tumor progression in patients with advanced gastric cancer. We herein review the clinical significance of CTC as a promising blood marker and therapeutic target in patients with gastric cancer.

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“…Since NASBA amplifies RNA using an RNA T7-polymerase promoter to generate multiple RNA products at 418C, double stranded DNA is not denatured and consequently not amplified ( Figure 1B) (Chan and Fox, 1999;Simpkins et al, 2000). NASBA has traditionally been used for the amplification of blood-borne viruses (Kievits et al, 1991;van Gemen et al, 1993), though it may also be useful for the detection of circulating tumour cells (Lambrechts et al, 1998(Lambrechts et al, , 1999. Potentially NASBA has two powerful advantages over RT -PCR for the molecular detection of circulating tumour cells.…”

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confidence: 99%

Comparison of the RNA-amplification based methods RT–PCR and NASBA for the detection of circulating tumour cells

Burchill

Perebolte²,

Johnston

et al. 2002

Br J Cancer

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Increasingly, reverse transcriptase polymerase chain reaction (RT–PCR) is used to detect clinically significant tumour cells in blood or bone marrow. This may result in a redefinition of disease-free and clinical relapse. However, its clinical utility may be limited by lack of automation or reproducibility. Recent studies have suggested nucleic acid sequence-based amplification of target RNA may be more robust. In this study, nucleic acid sequence-based amplification was established to detect melanoma, colorectal and prostate cancer cells. Nucleic acid sequence-based amplification and RT–PCR both successfully amplified target RNA in peripheral blood samples from patients with melanoma and colorectal cancer, but only RT–PCR detected PSA in blood samples from patients with prostate cancer. There was relatively good agreement between sample replicates analyzed by RT–PCR (Kappa values of one for tyrosinase, 0.67 for CK-20 and one for PSA), but less agreement when analyzed by nucleic acid sequence-based amplification. This may limit the routine use of NASBA for the detection of clinically significant disease. In summary, RT–PCR appears at present to be the most reliable and reproducible method for the detection of low-level disease in cancer patients, although prospective studies are warranted to assess the clinical utility of different molecular diagnostic methods. British Journal of Cancer (2002) 86 , 102–109. DOI: 10.1038/sj/bjc/6600014 www.bjcancer.com © 2002 The Cancer Research Campaign

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Comparison of immunocytochemistry, reverse transcriptase polymerase chain reaction, and nucleic acid sequence–based amplification for the detection of circulating breast cancer cells

Cited by 64 publications

References 33 publications

Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple mRNA markers

Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple mRNA markers

Clinical significance of circulating tumor cells in blood from patients with gastric cancer

Comparison of the RNA-amplification based methods RT–PCR and NASBA for the detection of circulating tumour cells

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