Comparison of immunocytochemistry and in situ hybridization in the cytodiagnosis of genital herpetic infection

Kobayashi, Tadao K.

doi:10.1002/dc.2840080110

Cited by 10 publications

(10 citation statements)

References 24 publications

(7 reference statements)

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“…168 Other herpesviruses (HHVs) may also be detected, including herpes simplex in lymphadenitis 169 and endometritis, 170 HHV-6 in erythroderma associated with an infectious mononucleosis-like syndrome, 171 in a variety of lymphoproliferative states, 172,173 and in AIDS-associated retinitis. 174 The presence of HHV-8 in Kaposi's sarcoma has been demonstrated using 175 There is some evidence to suggest that ISH may not be as sensitive as IHC in the detection of HSV, 176 but proper validation should be performed with the newer amplified techniques. It may have an advantage over IHC in early infection.…”

Section: Infectionsmentioning

confidence: 99%

In Situ hybridization and its diagnostic applications in pathology

1997

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In situ hybridization (ISH) is a technique by which specific nucleotide sequences are identified in cells or tissue sections. These may be endogenous, bacterial or viral, DNA or RNA. On the basis of research applications, the technique is now being translated into diagnostic practice, mainly in the areas of gene expression, infection and interphase cytogenetics. Diagnostic applications are most often based on short nucleotide sequences (oligomers) labelled with non‐isotopic reporter molecules, and sites of binding may be localized by histochemical or immunohistochemical methods. The technique can be applied to routinely fixed and processed tissues; with some targets, it is even possible to obtain hybridization in autopsy material. ISH has been used to detect messenger RNA (mRNA) as a marker of gene expression, where levels of protein storage are low; for example, to confirm an endocrine tumour as the source of excess hormone production. Its application in infectious diseases has to date been mainly in viral infections, such as the typing of human papillomavirus (HPV) or the detection of Epstein–Barr virus by the presence of small nuclear RNAs (EBERs). The expression of mRNAs for histone proteins has been used to detect cells in S phase, and related methods may be applied to detect apoptotic cells. Using probes to chromosome‐specific sequences, it is possible to detect aneuploidy, and to document changes in specific chromosomes, which may have prognostic significance in some tumours, such as B‐cell chronic lymphatic leukaemia. Using sequence‐specific probes, translocations can be identified, such as the t(11;12) of Ewing's sarcoma. This review presents an outline of the technique of in situ hybridization and discusses areas of current and potential diagnostic application. © 1997 John Wiley & Sons, Ltd.

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Section: Infectionsmentioning

confidence: 99%

In Situ hybridization and its diagnostic applications in pathology

1997

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“…** In the present case, we tested a commercially available biotinylated HSV D N A probe for detection of HSV genomic material in the nipple secretion instead of using immunocytochemistry. A recent study using cervical samples showed that the ISH may produce many nonspecific reaction in the cytoplasmic region, especially in squamous cells or scattered extracellular areas 22 and concluded that immunocytochemistry may be preferable to ISH for HSV confirmation in cervical specimens. However, in the present cases the ISH applied to nipple discharge revealed nonspecific staining extremely seldom when compared to those of cervical specimens.…”

Section: Discussionmentioning

confidence: 98%

“…24 ISH was used on the Papanicolaou-destained cellular samples which has been described previously. 22 …”

Section: Ish Techniquementioning

confidence: 97%

“…16,21,22 Cytological examination frequently provides valuable information in the evaluation of abnormal nipple discharge. It is generally believed that spontaneous nipple discharge in the nonlactating breast is an abnormal clinical sign that should be investigated.…”

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confidence: 99%

“…Tissue culture of virus remains the most sensitive and specific but requires a long period of time. More rapid methods of viral diagnosis in clinical samples include routine cytological examination for diagnostic viral inclusions and groundglass nuclei, electron microscopy, lo immunofluorescence staining, I ' immunocytochemical detection 6,9,12-14 of specific viral antigens and most recently, and nucleic acid Address reprint requests to Tadao K. Kobayashi, C.F.I.A.C., Department of Cytopathology, Saiseikai Shiga Hospital, Imperial Gift Foundation Inc. 2-4-1, Oh-Hashi, Ritto, Shiga 520-30, Japan.hybridization [15][16][17][18][19][20][21][22] for the demonstration of viral genetic information.Since the report of Brigati et al, l5 there has been an increased interest in the application of in situ hybridization techniques (ISH), applied, for example, to cytomegalovirus, '* adenovirus, l5 human papillomavirus, l9 human polyomavirus, 2o and HSV. 16,21,22 Cytological examination frequently provides valuable information in the evaluation of abnormal nipple discharge.…”

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confidence: 99%

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Cytologic detection of herpes simplex virus DNA in nipple discharge by in situ hybridization: Report of two cases

Kobayashi

Okamoto

Yakushiji

1993

Diagnostic Cytopathology

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Key Words: HSV DNA; Cytology; In Situ Hybridization; Nipple secretion; Breast milk Cellular morphology has been used an effective method for the diagnosis of herpes simplex virus (HSV) infection of" the genital tract and other body sites, 3-7 including ocular lesions. 5,8,9 Various methods for the diagnosis of herpesvirus infections are currently available. Tissue culture of virus remains the most sensitive and specific but requires a long period of time. More rapid methods of viral diagnosis in clinical samples include routine cytological examination for diagnostic viral inclusions and groundglass nuclei, electron microscopy, lo immunofluorescence staining, I ' immunocytochemical detection 6,9,12-14 of specific viral antigens and most recently, and nucleic acid Address reprint requests to Tadao K. Kobayashi, C.F.I.A.C., Department of Cytopathology, Saiseikai Shiga Hospital, Imperial Gift Foundation Inc. 2-4-1, Oh-Hashi, Ritto, Shiga 520-30, Japan.hybridization [15][16][17][18][19][20][21][22] for the demonstration of viral genetic information.Since the report of Brigati et al., l5 there has been an increased interest in the application of in situ hybridization techniques (ISH), applied, for example, to cytomegalovirus, '* adenovirus, l5 human papillomavirus, l9 human polyomavirus, 2o and HSV. 16,21,22 Cytological examination frequently provides valuable information in the evaluation of abnormal nipple discharge. It is generally believed that spontaneous nipple discharge in the nonlactating breast is an abnormal clinical sign that should be investigated. Pathological breast lesions associated with nipple discharge are usually benign, although cancer may occasionally present with nipple discharge. 23 To our knowledge, the cytologic finding of HSV infection in the nipple secretion has not been previously reported. This paper presents the cytologic findings of HSV infection using in situ hybridization. Case ReportsCase I A 36-yr-old women presented with a three weeks history of a blood stained discharge from the right breast. There were no associated symptoms and no family history of breast disease. However, the patient had experienced ulcerated, tender lesions on the lower lip 2 months before, thought to be herpetic gingivostomatitis. The patient's nipple revealed, crusting lesions with several group of vesicles on an inflammed base. Direct touch smears of the base of a ruptured vesicle on the nipple as well as nipple discharge showed characteristic multinucleated giant cells consistent with herpes simplex (Fig. C-1). Clinically, no HSV lesion was noted on the areola, and the other breast was normal. Nipple discharges were also submitted for 296

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Coexistence of Pemphigus vulgaris and herpes simplex virus infection in oral mucosa diagnosed by cytology, immunohistochemistry, and polymerase chain reaction

Takahashi¹,

Kobayashi²,

Suzuki³

et al. 1998

Diagn. Cytopathol.

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The patient was a 53-yr-old woman, who presented in September 1994 with blisters on her oral mucosa, and later, numerous bullous lesions appeared on her lips, face, and head. The patient was admitted to the dermatological ward for further investigations. Laboratory examination was not remarkable except for immunoglobulin, i.e., IgA 581 mg/dl and IgG 2,504 mg/dl, while IgM levels were unremarkable. At about the same time, a dermatologist established the

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Comparison of immunocytochemistry and in situ hybridization in the cytodiagnosis of genital herpetic infection

Cited by 10 publications

References 24 publications

In Situ hybridization and its diagnostic applications in pathology

In Situ hybridization and its diagnostic applications in pathology

Cytologic detection of herpes simplex virus DNA in nipple discharge by in situ hybridization: Report of two cases

Coexistence of Pemphigus vulgaris and herpes simplex virus infection in oral mucosa diagnosed by cytology, immunohistochemistry, and polymerase chain reaction

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