Comparison of immunoassay and gas chromatography–mass spectrometry for measurement of polycyclic aromatic hydrocarbons in contaminated soil

Chuang, Jane C.; Emon, Jeanette M. Van; Chou, Ying-Liang; Junod, Nadia; Finegold, J. K.; Wilson, Nancy K.

doi:10.1016/s0003-2670(03)00499-9

Cited by 35 publications

(17 citation statements)

References 11 publications

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“…Public awareness has resulted in many government and public authorities finding themselves under increased pressure to ensure that any discovery of contaminated soils is publicly disclosed and remediated quickly and effectively. Therefore, efficient techniques for sampling and analyses have become necessary (Chuang et al, 2003). Heterogeneity of soil properties is an issue confronted by many researchers, such as Besson et al in Chapter 23, and there is great difficulty in identifying both the extent of areas of contamination and the sources of the contaminants (Markus and McBratney, 1996).…”

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confidence: 99%

Diagnostic Screening of Urban Soil Contaminants Using Diffuse Reflectance Spectroscopy

Bray¹,

Rossel²,

McBratney³

2010

Proximal Soil Sensing

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There is increasing demand for cheap and rapid screening tests for soil contaminants in environmental consultancies. Diffuse infrared reflectance spectroscopy in the visible-near-infrared (vis-NIR) and mid-infrared (MIR) has the potential to meet this demand. The aims of this chapter are to develop diagnostic screening tests for heavy metals and polycyclic aromatic hydrocarbons (PAHs) in soil using vis-NIR and MIR diffuse reflectance spectroscopy. Cadmium, copper, lead, and zinc were analysed, as were total PAHs and benzo [a]pyrene. An ordinal logistic regression technique was used for predictions in the screening tests and to determine false-positive and false-negative rates. Zinc had the best prediction accuracy (89%) and copper predictions were consistently above 75%. Cadmium and lead were the least well predicted of the heavy metals (67 and 70%, respectively), and PAH predictions averaged 78.9%. MIR analysis (average prediction accuracy of 79.9%) was only slightly more accurate than vis-NIR analysis (average prediction accuracy of 77.1%), but the latter may currently be used in situ, thereby reducing cost and time of analysis and providing diagnosis in real time. Diffuse reflectance spectroscopy in the vis-NIR can substantially decrease both the time and the cost associated with screening for soil contaminants.

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mentioning

confidence: 99%

Diagnostic Screening of Urban Soil Contaminants Using Diffuse Reflectance Spectroscopy

Bray¹,

Rossel²,

McBratney³

2010

Proximal Soil Sensing

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“…The sampler can be used to collect cores without liners for immediate examination or with liners to obtain an undisturbed and sealed sample. The collected soil samples were analyzed using enzyme-linked immunosorbent assay (ELISA) method (Chuang et al 1998(Chuang et al , 2003Gilvanda et al 1998;Amin et al 1998;Watanabe et al 2004;and Musick et al 2000).…”

Section: Soil Sampling and Analysismentioning

confidence: 99%

Pesticide Impact Assessment via Using Enzyme-linked Immunosorbent Assay (ELISA) Technique in the Lower Rio Grande River Basin, Texas

et al. 2009

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This paper presents a field survey using health impact assessment approach trying to: (1) demonstrate a basic soil and groundwater survey/sampling, (2) investigate any possible long-term pesticide contamination in connection with citrus orchard pesticide application, and (3) conduct an assessment review of possible pesticide impacts on public health in the Lower Rio Grande Valley (LRGV), Texas. A sampling plan was prepared to collect samples that include soil from a citrus orchard of Texas A&M Agricultural Research and Extension Center, Weslaco, located in the Hidalgo county and groundwater from domestic and public wells located in the neighboring LRGV region. Field investigation of the contamination was performed using enzymelinked immunosorbent assay (ELISA) technique and the analytical results indicated that there is no considerable concentration of simazine pesticide in soil and groundwater. All of the measurements are below the maximum contamination limit (MCL) value provided by US EPA. A statistical assessment was also performed on the cancer mortality rates in the LRGV region to identify any significant health concerns in this region. After reviewing several relevant reports, it is inferred that there are no apparent health issues in the LRGV region in relation to such pesticide impact and

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“…Também, o uso de sistemas em fluxo acoplados a imunossensores não tem se mostrado uma tarefa fácil, e as pesquisas para o melhoramento dos sistemas em FIIA encontram, atualmente, seu foco nas seguintes vertentes: i) desenvolvimento de novos imunoreagentes (anticorpos mais específicos, para construção de sistemas multianalíticos); ii) estudos de novos formatos de EIA's, de modo a minimizar os inconvenientes relacionados com instabilidades dos reagentes e regeneração na imunosuperfície; iii) aplicação de metodologias já estabelecidas, como por ex., o uso de fibras óticas, para o desenvolvimento de sistemas miniaturizados e portáteis, e iv) desenvolvimento de imunosenssores capazes de determinar, simultaneamente, mais de um analito, através do uso de vários anticorpos e marcadores enzimáticos ao mesmo tempo 167 . Tais tecnologias poderão, no futuro, vir a ser a base para programas internacionais destinados ao controle "online" de poluição ambiental 168 . Um dos atuais grandes desafios no campo do desenvolvimento de imunoensaios é o estabelecimento de formatos que não empreguem marcadores.…”

Section: Avanços E Perspectivasunclassified

Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas

Nunes¹

2005

Quím. Nova

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Recebido em 6/2/04; aceito em 20/10/04; publicado na web em 28/2/05 IMMUNOCHEMICAL METHODS FOR ANALYSIS OF ENVIRONMENTAL CONTAMINANTS: CONCEPTS, STATE OF THE ART AND PERPECTIVES. Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzymelinked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC 50 ), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flowinjection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited.Keywords: immunoassays; environmental contaminants; monitoring. INTRODUÇÃOA origem dos métodos imunológicos situa-se no final da déca-da de 50, quando Yalow e Berson 1 descreveram o primeiro ensaio imunológico para detectar insulina humana, o que lhes rendeu o Prêmio Nobel em 1977. A partir de então, as técnicas imunoquí-micas foram desenvolvidas como métodos de análise de macromoléculas (enzimas, hormônios), e posteriormente para moléculas orgânicas pequenas (drogas, fármacos), tendo seu maior êxito no campo da química clínica 2-4 . Em meados da década de 70, foi desenvolvido o primeiro ensaio biológico para pesticidas (aldrin e dieldrin) 5 . A este ensaio, seguiram-se outros, também baseados na marcação radioativa, chamados ensaios radioimunológicos ("radioimmunoassay, RIA"). Os ensaios imunoenzimáticos ("enzyme immunoassay, EIA") surgiram na década de 60 para identificação e localização de antígenos em preparações histológicas 2,6,7 , tendo sido a sua utilidade reconhecida posteriormente em outros campos distintos [8][9][10][11][12][13] . No início dos anos 80, começou-se a discutir as possibilidades que estes tipos de métodos analíticos podiam oferecer na detecção de contaminantes, tanto em amostras ambientais como de alimentos 12,14-16 , o que fomentou a sua aplicação no campo da química ambiental. A partir desse momento, começou-se a utilizar a marcação enzimática, que proporcionava menores riscos e apresentava sensibilidade similar aos métodos convencionais de análise 12,[15][16][17][18][19] . Inúmeras vantagens podem ser obtidas com a utilização dos métodos imunoquímicos e, paulatinamente, vão sendo disponibilizadas informações sobre diversos aspectos analíticos, tais como i) interpretação dos dados obtidos no desenvolvimento dos métodos; ii) estratégias utilizadas para a garantia da qualidade dos procedimentos operacionais; iii) análise dos dados e das informa...

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Comparison of immunoassay and gas chromatography–mass spectrometry for measurement of polycyclic aromatic hydrocarbons in contaminated soil

Cited by 35 publications

References 11 publications

Diagnostic Screening of Urban Soil Contaminants Using Diffuse Reflectance Spectroscopy

Diagnostic Screening of Urban Soil Contaminants Using Diffuse Reflectance Spectroscopy

Pesticide Impact Assessment via Using Enzyme-linked Immunosorbent Assay (ELISA) Technique in the Lower Rio Grande River Basin, Texas

Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas

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