Comparison of immune peripheral blood cells in tuberculin reactor cattle that are seropositive or seronegative for Mycobacterium bovis antigens

Quevillon, Eve-Lyne; Díaz, Fernando; Jaramillo, Laura; Lascuraín, Ricardo; Gutiérrez‐Pabello, José A.; Castañeda, Felipe A.; Arriaga, C.; Pérez, Rafael Granell; González, Xochitl Eva González

doi:10.1016/j.vetimm.2013.02.016

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Previous studies stated that bovine have a dichotomous response to mycobacteria, that is, alternating high levels of antibody production with cellular immune response [35, 38, 50]. In line with this, we can infer that a humoral immune response was elicited in response to the fusion protein CMX and that a cellular response was directed only toward M. smegmatis , and thus, it was not observed in our assays.…”

Section: Discussionsupporting

confidence: 76%

“…2; and data not shown). This phenomena could be explained by a) the majority of the effector/ memory T cell being localized in the draining lymph nodes instead of in peripheral blood [26, 35], b) 30 days after the booster possibly being too long for the persistence of this population of cells in the periphery [26, 39] and c) CMX not having been presented by bovine antigen-presenting cells [22]. All of the above hypotheses must be further investigated.…”

Section: Discussionmentioning

confidence: 99%

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Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

Silva

Cavalcanti

Oliveira

et al. 2014

The Journal of Veterinary Medical Science

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This study aimed to evaluate the immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the CMX fusion protein composed of immunodominant epitopes Ag85C, MPT51 and HspX of Mycobacterium tuberculosis, which are important mycobacteria virulence factors. A group of Nelore heifers that were 10 to 12 months of age and negative for the tuberculin skin test (TST) were immunized with four doses of the recombinant vaccine mc2-CMX (M. smegmatis-Ag85C-MPT51-HspX) during a period of one year. Before each immunization, blood was collected to obtain sera for antibody analysis. Serological analysis demonstrated that mc2-CMX was able to induce a humoral response with increased levels of specific IgG antibodies against CMX, despite minimum antibody levels being detected for individual Ag85C, MPT51 or HspX recombinant antigens. However, there was no significant increase in specific CD4+ IFN-γ-positive T cells. Lymphadenomegaly was observed in superficial cervical lymph nodes adjacent to the site of vaccination among mc2-CMX-vaccinated bovines, and the histopathological analysis demonstrated follicular hyperplasia without inflammatory infiltrate or granuloma formation. Animals remained negative for the TST until the end of the experiments, showing no cross-reactivity with the recombinant vaccine and tuberculin proteins. We discuss the potential of mc2-CMX to induce an immune response in cattle.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

Silva

Cavalcanti

Oliveira

et al. 2014

The Journal of Veterinary Medical Science

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show abstract

“…Growth traits did not significantly differ between animals that seroconverted and those that did not, indicating that the development of the calves was not hindered by a lack of immune response up to the point at which serum samples were collected. Although individuals that did not seroconvert still grew normally and may be protected in herds due to herd immunity, these individuals may likely be the most at risk of viral infection (Quevillon et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of responses to vaccination of Angus cattle for four viruses that contribute to bovine respiratory disease complex1,2

Kramer

Mayes

Fritz-Waters

et al. 2017

Journal of Animal Science

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Although vaccination is an effective measure in reducing the risk of bovine respiratory disease complex (BRDC) in cattle, BRDC losses remain significant. Increasing the efficacy of vaccination depends on elucidating the protective immune response to different antigens included in vaccines, determining the best timing for vaccination, and understanding the impact of the age of the calf on vaccination. This study measured the serum antibodies present in calves following vaccination against 4 viruses commonly associated with BRDC: bovine viral diarrhea virus type 1 and 2 (BVDV1 and BVDV2), bovine respiratory syncytial virus (BRSV), and bovine herpesvirus 1 (BHV1). Serum antibody titers were measured in more than 1,600 calves at 3-wk intervals starting at the time of the first vaccination. This first vaccination occurred at weaning for approximately half of the individuals and 3 wk before weaning for the other half. Dam age (years), time of weaning (initial vaccination or booster vaccination), and age of calf within year-season (days within year-season) classification all were found to have a significant effect on measured traits such as the initial titer and overall response. An increased initial titer was negatively correlated with each response trait (initial, booster, and overall response). Calves that were weaned at initial vaccination had greater overall antibody response to BVDV1 and BVDV2 compared with calves weaned 3 wk before initial vaccination. In contrast, calves given their initial vaccination 3 wk before weaning had greater overall antibody response to BRSV and BHV1 compared with calves that were vaccinated at weaning. Furthermore, the circulating antibody titer at which each virus needed to be below for an individual calf to positively respond to vaccination was determined (log titer of 0.38 for BVDV1, 1.5 for BVDV2, 3.88 for BRSV, and 1.5 for BHV1). This information can be used to improve vaccination protocols to allow for a greater response rate of individuals to vaccination and, hopefully, improved protection.

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Fluoride induces immunotoxicity by regulating riboflavin transport and metabolism partly through IL-17A in the spleen

Qiao,

Cui,

Tan

et al. 2024

Journal of Hazardous Materials

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Comparison of immune peripheral blood cells in tuberculin reactor cattle that are seropositive or seronegative for Mycobacterium bovis antigens

Cited by 4 publications

References 40 publications

Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

Evaluation of responses to vaccination of Angus cattle for four viruses that contribute to bovine respiratory disease complex1,2

Fluoride induces immunotoxicity by regulating riboflavin transport and metabolism partly through IL-17A in the spleen

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