Comparison of immobilized and free enzyme systems in industrial production of short‐chain fructooligosaccharides from sucrose using a techno‐economic approach

Bedzo, Oscar K. K.; Mandegari, Mohsen; Görgens, Johann F.

doi:10.1002/bbb.2025

Cited by 12 publications

(47 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enzyme production in the same facility with scFOS was noted to enhance the economic performance of the plant. 27,40 Figure 3 shows the Aspen flowsheet of the scFOS plant.…”

Section: Succinic Acid (Sa)mentioning

confidence: 99%

“…Production medium and trace salts were added and maintained the growth of the cells until fermentation started, while ammonium hydroxide supplied nitrogen for the yeast and maintained the pH at 5 for maximum growth. 27 The fermentation mixture was flashed to remove gaseous effluents. Dead cells and unwanted proteins were removed from the supernatant by a centrifuge and ultrafiltration, respectively, to purify the enzyme.…”

Section: Succinic Acid (Sa)mentioning

confidence: 99%

“…40 The enzyme production was simulated using the RYield Aspen model at the optimal growth and protein expression temperature of 30 °C, specifying the desired enzyme yield. 27 FFase from the enzyme production stage was reacted with dilute A-molasses with sucrose concentration of 600 g/L at a temperature of 62 °C 27 using RYield reactor model in Aspen. The sucrose conversion was 92% with 58% scFOS yield on sucrose converted 18 and scFOS distribution of 38.8% GF 2 , 47.8% GF 3 and 13.4% GF 4 after 8 h. 39,41 The scFOS (GF 2 + GF 3 + GF 4 ) was separated from the reaction mixture containing 35.3% glucose, 18.6% fructose, 5.6% sucrose, 15.7% GF2, 19.4% GF3 and 5.4% GF4 using simulated moving bed chromatography (SMBC), at purity and yield of 90% and 97%.…”

Section: Succinic Acid (Sa)mentioning

confidence: 99%

“…18 They are produced by the degradation of inulin or transformation of sucrose by intracellular enzymes from bacteria, fungi or yeast sources. 27 Few techno-economic analyses were reported in the literature for the production of SA from crystalline sucrose 28 and lignocellulose 7 raw materials, and scFOS from crystalline sucrose. Efe et al 28 found that sucrose raw material had the highest contribution (29%) to the yearly operational cost of SA production and suggested the need for alternative raw materials such as molasses in the future, with suitable downstream processes to yield the required market purity.…”

mentioning

confidence: 99%

“…29 The techno-economic studies of scFOS production from pure crystalline sucrose in stand-alone plants focused on the types of enzyme systems and fermentations used rather than the raw material. 27,30 Currently, no techno-economic assessments have been reported for the production of either SA or scFOS from sugarcane molasses in an integrated biorefinery.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Dogbe

Mandegari

Görgens

2020

Biofuels Bioprod Bioref

Self Cite

View full text Add to dashboard Cite

The downward trend of global sugar market prices, as well as rising energy prices, threaten the survival of the South African sugar industry. Operating a single‐stage sugar crystallization process, which produces less crystalline sugar along with sucrose‐rich A‐molasses as a by‐product, to which value can be added in an integrated biorefinery, could be a viable solution for the industry. It would offer a diversification option as opposed to the current three‐stage crystallization process designed to achieve maximum sugar recovery. This study presents a rigorous Aspen Plus simulation development and economic assessment of different biorefinery scenarios producing succinic acid (SA) and short‐chain fructooligosaccharides (scFOS) from A‐molasses at target market sizes of 440 kt/year and 160 kt/year, respectively, considering the production capacity and market price interplays. In all, seven scenarios of single‐ or multi‐product(s) biorefinery annexed to an existing sugar mill were developed and assessed. Due to the integration benefits, all scenarios studied delivered an internal rate of return (IRR) between 24.1–61.1%, significantly above the required discount rate of 9.7%. In particular, co‐production of SA and scFOS syrup at production rates 40.5 kt/year and 13.5 kt/year from 70% and 30% of the A‐molasses, respectively resulted in the IRR of 56.1% and was considered the most attractive scenario based on the diverse bio‐production, economic performance and easy market entry of the products determined by low minimum selling prices. The proposed integration can lead to the sustainable operation of existing sugar mills by utilizing resources more efficiently, employment of product diversification and job creation. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

“…Enzyme production in the same facility with scFOS was noted to enhance the economic performance of the plant. 27,40 Figure 3 shows the Aspen flowsheet of the scFOS plant.…”

Section: Succinic Acid (Sa)mentioning

confidence: 99%

Section: Succinic Acid (Sa)mentioning

confidence: 99%

Section: Succinic Acid (Sa)mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Dogbe

Mandegari

Görgens

2020

Biofuels Bioprod Bioref

Self Cite

View full text Add to dashboard Cite

show abstract

Techno‐economic comparison of different reactor types used in the manufacture of fructooligosaccharides from sucrose

Vacharanukrauh,

Plubwungklam,

Pichyangkura

et al. 2023

Asia-Pacific J Chem Eng

View full text Add to dashboard Cite

Fructooligosaccharides, a low‐calorie alternative sweetener with prebiotic effects, can be synthesized from sucrose via enzymatic reactions. Recently, methods for producing novel fructooligosaccharides using free and immobilized Bacillus licheniformis RN‐01 levansucrase were developed at the laboratory scale. However, there was a need to apply engineering knowledge in production process design and scale‐up to develop and evaluate the feasibility of this technology in the industrial scale. In this paper, three different types of reactors for fructooligosaccharides production using B. licheniformis RN‐01 levansucrase were compared in terms of technical and economic aspects. The previously collected experimental data, including enzyme activities, immobilization yields, product yields, and material balances, were used to estimate the capital and operating costs of fructooligosaccharides production using (a) immobilized enzyme in a stirred‐tank reactor, (b) immobilized enzyme in a packed‐bed reactor, and (c) free enzyme in a stirred‐tank reactor. The technical issues in reactor design and operation were also evaluated. The results showed that, in terms of minimum product selling prices, the process using immobilized enzyme had more economic advantages than the process using free enzyme because the cost savings from enzyme purchasing could compensate the additional equipment and chemical costs in the enzyme immobilization process. The results could be a guideline for selecting suitable reactor types for fructooligosaccharides production and provide directions for further reactor design and development.

show abstract

Techno‐economic analysis of inulooligosaccharides, protein, and biofuel co‐production from Jerusalem artichoke tubers: A biorefinery approach

Bedzo

Mandegari

Görgens

2020

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Jerusalem artichoke (JA) is a crop with excellent potential for application in biorefineries. It can resist drought, pests, and diseases and can thrive well in marginal lands with little fertilizer application. The JA tubers contain considerable quantities of inulin, which is suitable for the production of inulooligosaccharides (IOS), as a high‐value prebiotic, dietary fiber. In this study, five JA tuber biorefinery scenarios were simulated in Aspen Plus® and further evaluated by techno‐economic and sensitivity analyses. Production of IOS, proteins and animal feed was studied in scenarios A and C, applying various biorefinery configurations. Scenario B explored the option of producing only IOS and the sale of residues as animal feed. Scenarios D and E investigated the economic potential of biofuel generation from residues after IOS and protein production by generation of biogas and ethanol respectively, from residues. Based on the chosen economic indicators, scenario B resulted in the lowest minimum selling price (MSP) of 3.91 US$ kg−1 (market price 5.0 US$ kg−1) with correspondingly reduced total capital investment (TCI) and total operating cost (TOC) per mass unit produced of IOS of 18.91 and 2.59 US$ kg−1 respectively, compared with other studied scenarios. Considering the set production scale, it is more profitable when the residues are sold as animal feed instead of being converted into biofuel, due to the capital‐intensive nature of the biofuel production processes. The coproduction of protein had a negative impact on the economics of the process as the associated capital and operating expenditure outweighed the associated revenue. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Comparison of immobilized and free enzyme systems in industrial production of short‐chain fructooligosaccharides from sucrose using a techno‐economic approach

Cited by 12 publications

References 42 publications

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Techno‐economic comparison of different reactor types used in the manufacture of fructooligosaccharides from sucrose

Techno‐economic analysis of inulooligosaccharides, protein, and biofuel co‐production from Jerusalem artichoke tubers: A biorefinery approach

Contact Info

Product

Resources

About