Comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk

Vithanage, Nuwan R.; Yeager, Thomas R.; Jadhav, Snehal; Palombo, Enzo A.; Datta, Nivedita

doi:10.1016/j.ijfoodmicro.2014.07.023

Cited by 60 publications

(53 citation statements)

References 84 publications

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“…Even though pseudomonads can secrete multiple proteases (Caballero et al, 2001;Stepaniak, Fox, & Daly, 1982) and multiple lipases (Beven, Dieckelmann, & Beacham, 2001;Machado et al, 2017), AprX and LipA have so far been considered to be the single, or the main, protease and lipase related to milk spoilage (Machado et al, 2017;Marchand et al, 2009b;Matéos et al, 2015;Vithanage, Yeager, Jadhav, Palombo, & Datta, 2014). In addition, both AprX and LipA are highly heat stable at UHT temperatures (Stoeckel et al, 2016b;Vithanage et al, 2016).…”

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Zhang

Bijl

Svensson

et al. 2019

Comp Rev Food Sci Food Safe

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The negative effects of proteases produced by psychrotrophic bacteria on dairy products, especially ultra‐high‐temperature (UHT) milk, are drawing increasing attention worldwide. These proteases are especially problematic, because it is difficult to control psychrotrophic bacteria during cold storage and to inactivate their heat‐resistant proteases during dairy processing. The predominant psychrotrophic species with spoilage potential in raw milk, Pseudomonas, can produce a thermostable extracellular protease, AprX. A comprehensive understanding of AprX on the aspects of its biological properties, regulation, proteolytic potential, and its impact on UHT milk can contribute to finding effective approaches to minimize, detect, and inactivate AprX. AprX also deserves attention as a representative of all extracellular metalloproteases produced by psychrotrophic bacteria in milk. The progress of current research on AprX is summarized in this review, including a view on the gap in current understanding of this enzyme. Reducing the production and activity of AprX has considerable potential for alleviating the problems that arise from the instability of UHT milk during shelf‐life.

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Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Zhang

Bijl

Svensson

et al. 2019

Comp Rev Food Sci Food Safe

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“…Some of the microbial groups linked to MIC include SRB, methanogens, sulphur-oxidising bacteria, nitratereducing bacteria, and iron-oxidising bacteria (Li et al, 2013). Genera representing the above-mentioned groups that have been studied using MALDI-TOF MS include Pseudomonas (Anderson et al, 2012;Seng et al, 2013;Vithanage, Yeager, Jadhav, Palombo, & Datta, 2014), Desulfovibrio (Barreau, Pagnier, & La Scola, 2013;Justesen et al, 2012;Seng et al, 2013), Desulfitobacterium (Krader & Emerson, 2004), Methanothermobacter and Methanococcus (Krader & Emerson, 2004), and Sphingomonas (Seng et al, 2013). Since the commercial databases were originally developed for the identification of clinically significant pathogens, they have incomplete representation of environmental microorganisms (Kopcakova et al, 2014).…”

Section: Proteomics and The Emergence Of Matrix-assisted Laser Desorpmentioning

confidence: 99%

Omics-based approaches and their use in the assessment of microbial-influenced corrosion of metals

Beale

Karpe²,

Jadhav

et al. 2016

Corrosion Reviews

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Microbial-influenced corrosion (MIC) has been known to have economic, environmental, and social implications to offshore oil and gas pipelines, concrete structures, and piped water assets. While corrosion itself is a relatively simple process, the localised manner of corrosion makes in situ assessments difficult. Furthermore, corrosion assessments tend to be measured as part of a forensic investigation. Compounding the issue further is the impact of microbiological/biofilm processes, where corrosion is influenced by the complex processes of different microorganisms performing different electrochemical reactions and secreting proteins and metabolites that can have secondary effects. While traditional microbiological culture-dependent techniques and electrochemical/physical assessments provide some insight into corrosion activity, the identity and role of microbial communities that are related to corrosion and corrosion inhibition in different materials and in different environments are scarce. One avenue to explore MIC and MIC inhibition is through the application of omics-based techniques, where insight into the bacterial population in terms of diversification and their metabolism can be further understood. As such, this paper discusses the recent progresses made in a number of fields that have used omics-based applications to improve the fundamental understanding of biofilms and MIC processes.

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“…Bacterial populations may change during food storage, and it is necessary to determine which bacteria are present at each stage of storage to select the most appropriate antimicrobial agents. Traditional methods of bacterial identification include culture‐based, biochemical, or serological tests and sequencing of the 16S rRNA gene, but these methods have a number of disadvantages . Matrix‐assisted laser desorption ionization time of flight mass spectrometry (MALDI‐TOF MS) can detect multiple different biomolecules and is a relatively powerful robust method for bacterial identification .…”

Section: Introductionmentioning

confidence: 99%

“…Traditional methods of bacterial identification include culture‐based, biochemical, or serological tests and sequencing of the 16S rRNA gene, but these methods have a number of disadvantages . Matrix‐assisted laser desorption ionization time of flight mass spectrometry (MALDI‐TOF MS) can detect multiple different biomolecules and is a relatively powerful robust method for bacterial identification . This technique is currently used extensively for clinical isolates and has good potential for the identification of food‐associated bacteria .…”

Section: Introductionmentioning

confidence: 99%

Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk

Bauer

Hayouka

2018

Journal of Peptide Science

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The shelf life of pasteurized bovine milk is limited by microorganism activity as surviving bacteria continue to grow in the bovine milk, eventually causing milk spoilage. In the current study, we used matrix-assisted laser desorption ionization time of flight mass spectrometry to identify pasteurized bovine milk-associated mesophilic and psychrotrophic bacteria. We have recently designed random cationic peptide mixtures that possess strong antimicrobial and antibiofilm properties. These compounds are cheap and easy to synthesize and represent a new class of antimicrobial agents. Here, we show that the random peptide mixtures are able to efficiently eradicate the bacteria identified as associated with pasteurized bovine milk, and reduced significantly the growth of Bacillus subtilis in milk. We propose these antimicrobial peptides as potential candidates for integration in bioactive milk and food packaging to prevent bacterial growth and extend the shelf life of food.

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Comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk

Cited by 60 publications

References 84 publications

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Omics-based approaches and their use in the assessment of microbial-influenced corrosion of metals

Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk

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