Comparison ofVibriospp. populations found in seawater, in exhibition aquaria, in fish intestine and in fish feed

Abstract

Aims: Vibrio populations in the seawater supply and the water of seven exhibition aquaria that simulate various Mediterranean and tropical ecosystems were compared. The similarity of Vibrio populations in the intestine of various fish species, feed and water was examined. Resistance to the antibiotics used in fish health management was analysed for the dominant Vibrio isolates. Methods and Results: Samples were collected for 1 year from seven exhibition tanks reproducing different ecosystems. The diversity and population similarity among vibrios were determined using a miniaturized biochemical phenotyping method. Similar Vibrio populations were found in the water supply and in the water of the Mediterranean ecosystems. However, different Vibrio populations were found in the water of tanks with tropical ecosystems. Vibrio populations in the water seemed to have a greater effect on the composition of intestinal Vibrio populations than those in feed. No resistance to antibiotics was observed, indicating their appropriate use for health management. Conclusions: Water characteristics have a greater impact on the composition of Vibrio populations in aquaria and fish intestinal microbiota than bacteria in feed. Significance and Impact of the Study: The microbiological monitoring water could provide valuable information for managing the health of exhibition aquaria.

Taxonomic and functional metagenomic profiling of gastrointestinal tract microbiome of the farmed adult turbot (Scophthalmus maximus)

“…To date, Vibrio was mainly isolated from turbot skin, larval turbot gut and turbot‐associated seawater (Montes et al ., , ; Cerdà‐Cuéllar & Blanch, ). Many surveys also demonstrated that Vibrio was dominant in marine fish gut (Blanch et al ., ; Smriga et al ., ). In this study, this genus was observed in all parts of turbot GI tract, especially in rectum content (Fig.…”

“…To date, most knowledge in relation to the normal GI microbiome of fish was based on the use of culture‐dependent and 16S rRNA gene‐based methods. The bacterial community of freshwater or marine fish GI tract seemed to be dominated by Proteobacteria and Firmicutes (Blanch et al ., ; Smriga et al ., ). Several special groups, such as probiotics, pathogens and cellulose‐decomposing bacteria, were also isolated from various freshwater or marine fish species (Vine et al ., ; Lazado et al ., ; Pérez‐Sánchez et al ., ; Wu et al ., ).…”

Community assembly of a euryhaline fish microbiome during salinity acclimation

“…Vibrio has been previously shown to occur at high abundance in fish (Austin & Austin ; Blanch et al . ; Sullam et al . ; Xing et al .…”

Associations and dynamics of Vibrionaceae in the environment, from the genus to the population level

“…The ability of Vibrio representatives to resist low pH and bile supports their survival within the fish gut (Yoshimizu and Kimura, 1976). Whether food or water intake is the greater source of inoculation is an open question: some studies have found a strong effect of food source on gut Vibrio composition (e.g., Grisez et al, 1997), whereas others found a stronger influence of Vibrio representation in the water column (e.g., Blanch et al, 2009). Conversely, Vibrio content of the fish gut has also been shown to be responsible for increasing Vibrio abundance in surrounding water when fish were introduced into a tank that did not otherwise support Vibrio growth, demonstrating significant excretion of viable cells from the fish gut (Sugita et al, 1985).…”