Comparison of <i>TRY</i> and the closely related <i>At1g01380</i> gene in controlling Arabidopsis trichome patterning

Esch, Jeffrey J.; Chen, Margaret A.; Hillestad, Matthew L.; Marks, M. David

doi:10.1111/j.1365-313x.2004.02259.x

Cited by 121 publications

(123 citation statements)

References 34 publications

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“…Both CPC and TRY encode R3 MYB proteins missing a domain that activates transcription (Wada et al, 1997;Schellmann et al, 2002). It has been proposed that R3 MYB proteins compete with R2R3 MYB transcription factors for binding to bHLH transcription factors to inhibit the formation of the regulatory complex (Esch et al, 2003(Esch et al, , 2004. Accumulation of CPC and TRY proteins should inhibit the formation of WER-GL3/EGL3-TTG1 complexes and repress TTG2 expression in H cells of wild-type roots and in all epidermal root cells of Pro35S:CPC plants.…”

Section: Ttg2 Expression Is Regulated By Myb Bhlh and Ttg1mentioning

confidence: 99%

Arabidopsis TRANSPARENT TESTA GLABRA2Is Directly Regulated by R2R3 MYB Transcription Factors and Is Involved in Regulation ofGLABRA2Transcription in Epidermal Differentiation

et al. 2007

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Arabidopsis thaliana TRANSPARENT TESTA GLABRA2 (TTG2) encodes a WRKY transcription factor and is expressed in young leaves, trichomes, seed coats, and root hairless cells. An examination of several trichome and root hair mutants indicates that MYB and bHLH genes regulate TTG2 expression. Two MYB binding sites in the TTG2 59 regulatory region act as cis regulatory elements and as direct targets of R2R3 MYB transcription factors such as WEREWOLF, GLABRA1, and TRANSPARENT TESTA2. Mutations in TTG2 cause phenotypic defects in trichome development and seed color pigmentation. Transgenic plants expressing a chimeric repressor version of the TTG2 protein (TTG2:SRDX) showed defects in trichome formation, anthocyanin accumulation, seed color pigmentation, and differentiation of root hairless cells. GLABRA2 (GL2) expression was markedly reduced in roots of ProTTG2:TTG2:SRDX transgenic plants, suggesting that TTG2 is involved in the regulation of GL2 expression, although GL2 expression in the ttg2 mutant was similar to that in the wild type. Our analysis suggests a new step in a regulatory cascade of epidermal differentiation, in which complexes containing R2R3 MYB and bHLH transcription factors regulate the expression of TTG2, which then regulates GL2 expression with complexes containing R2R3 MYB and bHLH in the differentiation of trichomes and root hairless cells.

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Section: Ttg2 Expression Is Regulated By Myb Bhlh and Ttg1mentioning

confidence: 99%

Arabidopsis TRANSPARENT TESTA GLABRA2Is Directly Regulated by R2R3 MYB Transcription Factors and Is Involved in Regulation ofGLABRA2Transcription in Epidermal Differentiation

et al. 2007

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“…2004; Serna and Martin 2006). Major regulators of trichome development include positive and negative regulators.…”

Section: Introductionmentioning

confidence: 99%

Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China

Ning

Wang

Zhou

et al. 2016

Ecology and Evolution

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Caragana korshinskii is commonly employed to improve drought ecosystems on the Loess Plateau, although the molecular mechanism at work is poorly understood, particularly in terms of the plant's ability to tolerate drought stress. Water is the most severe limiting factor for plant growth on the Loess Plateau. The trichome is known to play an efficient role in reducing water loss through decreasing the rate of transpiration, so in this study, we focused on the trichome‐related gene expression of ecological adaptation in C. korshinskii under low precipitation conditions. In order to explore the responses of trichomes to drought, we selected two experimental sites from wet to dry along the Loess Plateau latitude gradient for observation. Micro‐phenomena through which trichomes grew denser and larger under reduced precipitation were observed using a scanning electron microscope; de novo transcriptomes and quantitative PCR were then used to explore and verify gene expression patterns of C. korshinskii trichomes. Results showed that GIS2,TTG1, and GL2 were upregulated (as key positive‐regulated genes on trichome development), while CPC was downregulated (negative‐regulated gene). Taken together, our data indicate that downstream genes of gibberellin and cytokinin signaling pathways, alongside several cytoskeleton‐related genes, contribute to modulating trichome development to enhance transpiration resistance ability and increase the resistance to drought stress in C. korshinskii.

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“…TTG1, GL1, and GL3+EGL3 encode a WD40 protein, an R2R3 MYB transcription factor, and two basic helix-loop-helix-type transcription factors, respectively, and they form a ternary complex that initiates trichome cell development by activating GL2, which encodes a homeodomain/leucine zipper t ranscription factor (Rerie et al, 1994;Masucci et al, 1996;Schiefelbein, 2003;Pesch and Hü lskamp, 2004;Ramsay and Glover, 2005). Another group of genes encode the single-repeat R3 MYB factors TRIPTYCHON (TRY), CAPRICE (CPC), ENHANCER OF TRY AND CPC1 (ETC1), ETC2, ETC3, and TRICHOMELESS1 (TCL1) (Wada et al, 1997;Esch et al, 2004;Kirik et al, 2004aKirik et al, , 2004bSchellmann et al, 2002;Simon et al, 2007;Wang et al, 2007). They suppress trichome initiation in a redundant manner, although TRY, TCL1, and CPC exert a major influence on rosette leaves, inflorescences, and roots (root hairs), respectively (Wada et al, 1997;Schnittger et al, 1998;Schellmann et al, 2002;Wang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Temporal Control of Trichome Distribution by MicroRNA156-TargetedSPLGenes inArabidopsis thaliana

et al. 2010

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The production and distribution of plant trichomes is temporally and spatially regulated. After entering into the flowering stage, Arabidopsis thaliana plants have progressively reduced numbers of trichomes on the inflorescence stem, and the floral organs are nearly glabrous. We show here that SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes, which define an endogenous flowering pathway and are targeted by microRNA 156 (miR156), temporally control the trichome distribution during flowering. Plants overexpressing miR156 developed ectopic trichomes on the stem and floral organs. By contrast, plants with elevated levels of SPLs produced fewer trichomes. During plant development, the increase in SPL transcript levels is coordinated with the gradual loss of trichome cells on the stem. The MYB transcription factor genes TRICHOMELESS1 (TCL1) and TRIPTYCHON (TRY) are negative regulators of trichome development. We show that SPL9 directly activates TCL1 and TRY expression through binding to their promoters and that this activation is independent of GLABROUS1 (GL1). The phytohormones cytokinin and gibberellin were reported to induce trichome formation on the stem and inflorescence via the C2H2 transcription factors GIS, GIS2, and ZFP8, which promote GL1 expression. We show that the GIS-dependent pathway does not affect the regulation of TCL1 and TRY by miR156-targeted SPLs, represented by SPL9. These results demonstrate that the miR156-regulated SPLs establish a direct link between developmental programming and trichome distribution.

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Comparison of TRY and the closely related At1g01380 gene in controlling Arabidopsis trichome patterning

Cited by 121 publications

References 34 publications

Arabidopsis TRANSPARENT TESTA GLABRA2Is Directly Regulated by R2R3 MYB Transcription Factors and Is Involved in Regulation ofGLABRA2Transcription in Epidermal Differentiation

Arabidopsis TRANSPARENT TESTA GLABRA2Is Directly Regulated by R2R3 MYB Transcription Factors and Is Involved in Regulation ofGLABRA2Transcription in Epidermal Differentiation

Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China

Temporal Control of Trichome Distribution by MicroRNA156-TargetedSPLGenes inArabidopsis thaliana

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