Comparison of Hydrostatic Filtration Dialysis with Ultracentrifugation Methods for the Identification and Proteomic Profiling of Urinary Extracellular Vesicles

Chen, Yunying; Hong, Guobao; Wu, Fan; Sheng, Jieli; Zou, Zhong‐Mei; Xiong, Chengliang; Zhang, Ying; Deng, Jin; Tang, Hongjuan; Wang, Xin; Zou, Hequn

doi:10.7754/clin.lab.2018.180922

Cited by 3 publications

(3 citation statements)

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“…In detail, Chen et al. developed a new hydrostatic filtration for EV isolation, and they confirmed that this new method has a good consistency (>85% overlap) with the traditional DUC‐based EV isolation through MS‐based proteomic profiling [62]. Similarly, Nordin et al.…”

Section: Extracellular Vesiclesmentioning

confidence: 95%

Proteomic profiling in extracellular vesicles for cancer detection and monitoring

et al. 2021

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Extracellular vesicles (EVs) are nanometer-size lipid vesicles released by cells, which play essential biological functions in intercellular communication. Increasing evidence indicates that EVs participate in cancer development, including invasion, migration, metastasis, and cancer immune modulation. One of the key mechanisms is that EVs affect different cells in the tumor microenvironment through surface-anchor proteins and protein cargos. Moreover, proteins specifically expressed in tumor-derived EVs can be applied in cancer diagnosis and monitoring. Besides, the EV proteome also helps to understand drug resistance in cancers and to guide clinical medication. With the development of mass spectrometry and array-based multi-protein detection, the research of EV proteomics has entered a new era. The high-throughput parallel proteomic profiling based on these new platforms allows us to study the impact of EV proteome on cancer progression more comprehensively and to describe the proteomic landscape in cancers with more details. In this article, we review the role and function of different types of EVs in cancer progression. More importantly, we summarize the proteomic profiling of EVs based on different methods and the application of EV proteome in cancer detection and monitoring.

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Section: Extracellular Vesiclesmentioning

confidence: 95%

Proteomic profiling in extracellular vesicles for cancer detection and monitoring

et al. 2021

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“…Hydrostatic filtration dialysis (HFD) is another method that was previously presented by several groups and found to have benefits for isolation of EVs from urine (Table 1). [79][80][81][82][83] In this approach, a dialysis cellulose ester membrane with 1000 kDa cutoff is typically used and connected with a funnel. The urine sample passes through the membrane due to hydrostatic pressure.…”

Section: Hydrostatic Filtration Dialysismentioning

confidence: 99%

Filtration-based technologies for isolation, purification and analysis of extracellular vesicles

Chernyshev,

Yashchenok,

Ivanov

et al. 2023

Phys. Chem. Chem. Phys.

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“…Examples such as the ultrafiltration system and tangential flow filtration (TFF) use membranes with a defined molecular weight cut-off ranging from 10 to 100 kDa and have been used to isolate EVs from urine-derived cell culture medium [125,126]. Another method is the size-exclusion chromatography (SEC) that separates EVs from different body fluids based on their hydrodynamic volume and molecular size [128]. The combination of two SEC columns (2D SEC) was recently shown to improve the isolation capacity of the different urinary exosome subpopulations [131].…”

Section: New Technologies For Evs Isolationmentioning

confidence: 99%

Extracellular Vesicles as a Therapeutic Tool for Kidney Disease: Current Advances and Perspectives

Corrêa

Juncosa

Masereeuw

et al. 2021

IJMS

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Extracellular vesicles (EVs) have been described as important mediators of cell communication, regulating several physiological processes, including tissue recovery and regeneration. In the kidneys, EVs derived from stem cells have been shown to support tissue recovery in diverse disease models and have been considered an interesting alternative to cell therapy. For this purpose, however, several challenges remain to be overcome, such as the requirement of a high number of EVs for human therapy and the need for optimization of techniques for their isolation and characterization. Moreover, the kidney’s complexity and the pathological process to be treated require that EVs present a heterogeneous group of molecules to be delivered. In this review, we discuss the recent advances in the use of EVs as a therapeutic tool for kidney diseases. Moreover, we give an overview of the new technologies applied to improve EVs’ efficacy, such as novel methods of EV production and isolation by means of bioreactors and microfluidics, bioengineering the EV content and the use of alternative cell sources, including kidney organoids, to support their transfer to clinical applications.

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Comparison of Hydrostatic Filtration Dialysis with Ultracentrifugation Methods for the Identification and Proteomic Profiling of Urinary Extracellular Vesicles

Cited by 3 publications

References 0 publications

Proteomic profiling in extracellular vesicles for cancer detection and monitoring

Proteomic profiling in extracellular vesicles for cancer detection and monitoring

Filtration-based technologies for isolation, purification and analysis of extracellular vesicles

Extracellular Vesicles as a Therapeutic Tool for Kidney Disease: Current Advances and Perspectives

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