Comparison of Hungarian and Bulgarian isolates of maize dwarf mosaic virus

Tóbiás, István; Bakardjieva, N.; Palkovics, László

doi:10.1556/crc.35.2007.4.11

Cited by 5 publications

(5 citation statements)

References 11 publications

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“…The use of the CP gene for the generation of MDMV phylogenies, may be appropriate in cases where complete genomes are not available, due to the congruency observed between the CP and complete genome trees from this study. The CP gene of plant viruses has been used in many evolutionary studies in the past because the N terminal region is very informative with high genomic variability 14,26,[33][34][35] , while the core region is more stable, providing enough information in understanding MDMV evolution 25 . In the case of MDMV, the CP gene is evolutionary more informative with the 39 nt insertion as shown in Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary study of maize dwarf mosaic virus using nearly complete genome sequences acquired by next-generation sequencing

Wijayasekara

Ali

2021

Sci Rep

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Next-generation sequencing is a robust approach to sequence plant virus genomes in a very short amount of time compared to traditional sequencing methods. Maize dwarf mosaic virus (MDMV) is one of the most important plant viruses worldwide and a significant threat to maize production. In this study, we sequenced 19 MDMV isolates (10 from Johnsongrass and 9 from maize) collected in Oklahoma and Missouri during 2017–2019 using Illumina sequencing and determined the genetic diversity. Sequence reads were assembled and 19 nearly complete genome sequences of MDMV isolates were obtained. Phylogenetic analysis based on complete genomes nucleotide and amino acid sequences revealed two main clusters and a close evolutionary relationship among 19 MDMV isolates. Statistical analysis of individual genes for site-specific selection revealed that all genes are under negative selection. The fixation index (FST) analysis of the MDMV isolates revealed no gene flow between the two main phylogenetic clusters, which emphasizes the divergence of MDMV isolates from the USA. Among the USA MDMV isolates, the mean genetic distance (d) and nucleotide diversity ((π) were highest in the P1 gene coding region. This is the first detailed study on the evolutionary relationship of MDMV isolates based on the nearly complete genome analysis from maize and Johnsongrass.

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Section: Discussionmentioning

confidence: 99%

Evolutionary study of maize dwarf mosaic virus using nearly complete genome sequences acquired by next-generation sequencing

Wijayasekara

Ali

2021

Sci Rep

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“…Maize dwarf mosaic virus (MDMV) is one of the most important microbial stressors of sweet corn varieties. The infection often causes a crop loss of 10-45%, but the damage may reach up to 100% (Oertel et al 1997;Tóbiás et al 2007). MDMV preferentially colonizes members of the Poaceae family and is spread via aphids, pollen, and seed transmission (Urcuqui-Inchima et al 2001;Gell et al 2010;Stewart et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Protective role of S-methylmethionine-salicylate in maize plants infected with Maize dwarf mosaic virus

et al. 2017

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This study aimed to detect the harmful effects of Maize dwarf mosaic virus (MDMV) infection, and to demonstrate the potential benefits of S-methylmethionine-salicylate (MMS) pretreatment in infected maize (Zea mays L.) plants. The results of chlorophyll a fluorescence measurements showed that in MDMV-infected plants additional quenchers of fluorescence appear, probably as the result of associations between the virus coat protein and thylakoid membranes. It is important to note that when infected plants were pretreated with MMS, such associations were not formed. MDMV infection and MMS pretreatment resulted in a decrease in ascorbate peroxidase (APX) activity in maize leaves, while infection contributed to an increase in activity in the roots. Infection raised the guaiacol peroxidase (GPX) enzyme activity level, which was reduced by MMS pretreatment. MMS contributed to a decrease in both the RNA and coat protein content of MDMV, to an equal extent in maize leaves and roots. The results showed that MMS pretreatment enhanced the stress response reactions against MDMV infection in maize plants and retarded the spreading of infection.

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“…Maize dwarf mosaic virus (MDMV) is one of the most important biotic stressors of cultivated sweet corn varieties. The infection usually causes a crop loss of 10-45 %, but the damage may be as high as 100 % (Tóbiás et al 2007). Around 2/3 of sweet corn varieties are susceptible to MDMV infection, and even young resistant plants (in the 2-3-leaf stage) may be infected (Williams and Pataky 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Around 2/3 of sweet corn varieties are susceptible to MDMV infection, and even young resistant plants (in the 2-3-leaf stage) may be infected (Williams and Pataky 2012). The virus preferentially colonizes members of the Poaceae family and spreads in natural or agro-environments via aphid, pollen, and seed transmission (UrcuquiInchima et al 2001;Tóbiás et al 2007;Stewart et al 2012). During viral replication, MDMV infects organelles, especially chloroplasts, therefore their loss of function can be observed in response to viral infection (Mayhew and Ford 1974;Hammond 1998;Wei et al 2010).…”

Section: Introductionmentioning

confidence: 99%

S-methylmethionine contributes to enhanced defense against Maize dwarf mosaic virus infection in maize

et al. 2015

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The beneficial and protective effects of Smethylmethionine (SMM) were investigated in Maize dwarf mosaic virus (MDMV) infected maize (Zea mays L.). Response reactions and alterations in the physiological state of the plants were monitored by following changes in the values of chlorophyll a fluorescence and chlorophyll content, and differences in the expression patterns of the stress-related genes S-adenosylmethionine synthase (SAMS) and the 14-3-3-like protein gene G-box factor 14-6 (GF14-6). Infection was validated using the ELISA technique. A pronounced decrease in the red to far-red chlorophyll a fluorescence ratio, indicative of chlorophyll content, was observed in infected plants, which was tempered by SMM pretreatment. A noticeable decrease in the photochemical quenching of photosystem II and the thermal dissipation of the antennae was observed, together with a notable increase in other non-photochemical energy dissipation parameters in response to MDMV infection. SMM treatment enhanced blue fluorescence in both uninfected and MDMV-infected plants (probably due to the production of protective phenolic compounds), while infection characteristically increased green fluorescence emission. SMM treatment was found to elevate the rate of gene expressions of SAMS and GF14-6. The results suggest that SMM pretreatment enhances the stress response reactions that protect maize plants against MDMV infection.

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Comparison of Hungarian and Bulgarian isolates of maize dwarf mosaic virus

Cited by 5 publications

References 11 publications

Evolutionary study of maize dwarf mosaic virus using nearly complete genome sequences acquired by next-generation sequencing

Evolutionary study of maize dwarf mosaic virus using nearly complete genome sequences acquired by next-generation sequencing

Protective role of S-methylmethionine-salicylate in maize plants infected with Maize dwarf mosaic virus

S-methylmethionine contributes to enhanced defense against Maize dwarf mosaic virus infection in maize

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