Comparison of human and chimpanzee Kell blood group systems

Redman, Colvin M.; Lee, S.; Huinink, Daan ten Bokkel; Rabin, B. I.; Johnson, C.L.; Øyen, Ragnhild; Marsh, W. L.

doi:10.1046/j.1537-2995.1989.29689318444.x

Cited by 16 publications

(4 citation statements)

References 11 publications

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“…Chimpanzee's red cells also carry KEL6 antigens [31] and RFLP analysis of DNA from a single chimpanzee, by digestion of PCR products with MnlI and DdeI, indicated that the chimpanzee had the same base substitutions as noted in KEL6/6 humans.…”

Section: Molecular Basis Of Kell Blood Group Phenotypesmentioning

confidence: 93%

Molecular Basis of Kell Blood Group Phenotypes

Lee¹

1997

Vox Sanguinis

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The molecular basis of different Kell blood group phenotypes is reviewed. Sequence analysis of the Kell gene (KEL) established that single base substitutions, resulting in amino acid changes, are responsible for the different phenotypes. Most of the amino acid substitutions, with the exception of the one responsible for expression of KEL6 (Js^a), occur at the amino-terminal half of the protein, a domain that has least amino acid homology with a family of zinc endopeptidases, which include neutral endopeptidase 24.11 and endothelin-converting enzyme-1. Some of the genes were expressed in transfected cells and typed with alloantibodies to confirm that the identified mutations are responsible for antigen expression. Clinical applications of Kell blood group genotyping which include prenatal diagnosis to monitor hemolytic disease of the newborn are discussed.

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Section: Molecular Basis Of Kell Blood Group Phenotypesmentioning

confidence: 93%

Molecular Basis of Kell Blood Group Phenotypes

Lee¹

1997

Vox Sanguinis

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“…It is a complex system containing at least 24 antigens (1, 2) residing on a 93,000-kDa glycoprotein that is surface-exposed, spans the erythrocyte membrane, and is attached to the underlying cytoskeleton (3,4). Kell glycoproteins contain =12% carbohydrate by weight, all of which are N-linked glycosides (5,6).…”

mentioning

confidence: 99%

Molecular cloning and primary structure of Kell blood group protein.

Lee

Zambas

Marsh

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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204

125

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The Kell blood group is a major antigenic system in human erythrocytes. Kell antigens reside on a 93-kDa membrane glycoprotein that is surface-exposed and associated with the underlying cytoskeleton. We isolated tryptic peptides and, based on the amino acid sequence of one of the peptides and by using the PCR, prepared a specific oligonucleotide to screen a AgtlO human bone-marrow cDNA library. Four clones were isolated, one containing cDNA with an open reading frame for an 83-kDa protein. Al known Kell amino acid sequences were present in the deduced sequence; moreover, rabbit antibody to a 30-amino acid peptide, prepared from this sequence, reacted on an immunoblot with authentic Kell protein. The Kell cDNA sequence predicts a 732-amino acid protein. Hydropathy analysis indicates a single membranespanning region, suggesting that Kell protein is oriented with 47 of its N-terminal amino acids in the cell cytoplasm, and a 665-amino acid segment, which contains six possible N-glycosylation sites, is located extracellularly. Computer-based search showed that Kell has structural and sequence homology to a family of zinc metalloglycoproteins with neutral endopeptidase activity.The Kell blood group system is important in transfusion medicine. It is a complex system containing at least 24 antigens (1, 2) residing on a 93,000-kDa glycoprotein that is surface-exposed, spans the erythrocyte membrane, and is attached to the underlying cytoskeleton (3,4

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“…One has to notice that this Mab was unreactive with chimpanzee RBCs. Although it was demonstrated that chimpanzee RBCs were agglutinated by mouse Mabs reacting with human Kell-related antigens [20], the macaque Mab P.Mac-l2/1F9 failed to react with any chimpanzee RBC sample.…”

Section: Pnmac-8b/2e4mentioning

confidence: 97%

Anti‐human red cell monoclonal antibodies produced by macaque–mouse heterohybridomas: Their reactivity with human and nonhuman primate erythrocytes

Blancher

Roubinet²,

Reld

et al. 1999

J of Medical Primatology

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Eighteen monoclonal antibodies (Mabs) against human red blood cells (RBCs) produced by macaque mouse heterobybridomas gave uniformly positive reactions with all human samples except for some with particular null phenotypes. Based on reactions with latter cells, the human antigenic targets of 11 antibodies could be identified: six were specific for glycophorin-related antigens (Wr(b), En(a), Ge4), and each of the live remaining antibodies showed one of the following specificities: CD55, CD44, CD59, Kell, and Rh proteins. Four Mabs recognized the Vc antigen of the chimpanzee V-A-B-D system. Six macaque Mabs detected polymorphisms in chimpanzee, gorilla, orangutan, and gibbon that did not correspond to any known blood group in these animals, while other Mabs gave monomorphic reactions with ape RBCs. The reagents produced by macaque hybridomas are useful tools not only for human blood grouping tests, but also for tracing the relationships among blood group antigens of man and anthropoid apes.

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Comparison of human and chimpanzee Kell blood group systems

Cited by 16 publications

References 11 publications

Molecular Basis of Kell Blood Group Phenotypes

Molecular Basis of Kell Blood Group Phenotypes

Molecular cloning and primary structure of Kell blood group protein.

Anti‐human red cell monoclonal antibodies produced by macaque–mouse heterohybridomas: Their reactivity with human and nonhuman primate erythrocytes

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