Comparison of human and bovine enamel in a microbial caries model at different biofilm maturations

Ayoub, Hadeel M.; Gregory, Richard L.; Tang, Qing; Lippert, Frank

doi:10.1016/j.jdent.2020.103328

Cited by 16 publications

(9 citation statements)

References 38 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even if we consider the small decrease in color depth as a reduction of adsorbed macromolecules, no correlations between adhesion force and adsorbed amount or rupture length and adsorbed amount were found. This is probably due to the fact that variations in adhesion forces and rupture lengths are quite large between individual bacteria, depending on the study design. ,,, The data shows that small changes in the thickness of the film do not influence the adhesion process significantly.…”

Section: Resultsmentioning

confidence: 90%

“…This is probably due to the fact that variations in adhesion forces and rupture lengths are quite large between individual bacteria, depending on the study design. 17,40,61,49 The data shows that small changes in the thickness of the film do not influence the adhesion process significantly. Sample Characterization.…”

Section: ■ Materials and Methodsmentioning

confidence: 92%

“…Throughout this study, three pieces of enamel cut from the vestibular surface of three bovine incisor teeth from different animals were used. Bovine dental hard substance has been an established substitute for healthy human teeth in dental studies for many decades. − Similar to the HAp sample, the enamel was polished in several steps before usage except for the polishing solution, where a suspension of 40 nm sized colloidal silica particles (OP-S, Struers, Ballerup, Denmark, rebranded: now OP-U) was used. Residues were removed in an ethanol ultrasonic bath.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study

Mischo

Faidt

McMillan

et al. 2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration, and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of cell wall proteins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples, and the change in the macromolecules adsorbed over time on the surface. We found that the adhesion properties of S. aureus were similar on HAp and enamel samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

show abstract

Section: Resultsmentioning

confidence: 90%

Section: ■ Materials and Methodsmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study

Mischo

Faidt

McMillan

et al. 2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Additionally, it has been observed that the use of this substrate in the microbial caries model showed similar behavior to human teeth. 31 To suspend or minimize the structural loss in high-risk caries, an experimental chitosan/casein treatment was employed, as literature has shown that casein, a milk protein, is similar in function to proteins responsible for the biomineralization of the tooth. 20,32 Casein phosphopeptide, in particular, can bind to surfaces such as plaque, soft tissue and dentin, as well as provide a reservoir of bio-available calcium and phosphate in the saliva and on the surface of dental structure.…”

Section: Discussionmentioning

confidence: 99%

Effect of an experimental chitosan/casein gel on demineralized enamel under a cariogenic challenge

et al. 2022

View full text Add to dashboard Cite

Background. Dental caries is considered one of the most common oral health diseases.Objectives. The aim of the study was to evaluate the effects of an experimental chitosan/casein gel on enamel demineralization/remineralization in an environment with a high cariogenic challenge. Material and methods.Thirty-six specimens of bovine enamel (4 mm × 3 mm × 2 mm) were ground flat and polished. Then, the specimens were immersed in acetate buffer for 43 h with half of the surface protected (serving as control) and the other half exposed. All demineralized surfaces were randomly assigned into 3 groups (n = 12 per group) according to the type of treatment (G1 -control, G2 -1.5% chitosan gel with 1.5% casein, and G3 -1.5% chitosan gel without casein), and the corresponding treatment was applied once a week for 3 weeks. The specimens were also subjected to pH cycles of demineralization/ remineralization and the treatments were performed 3 times at 7-day intervals for a total of 21 days. Surface images were obtained for the analysis of initial roughness and, after the cariogenic challenge, new images were obtained to evaluate the final roughness, volume loss and wear profile using laser confocal microscopy. After the analyses, the specimens were cut and the depth of demineralization was measured. The data were analyzed using the Kruskal-Wallis analysis of variance (ANOVA) and the Tukey's test.Results. While the chitosan gel with casein showed a similar loss to the control group (p > 0.05), both gels resulted in similar volume loss (p > 0.05). There were no statistical differences regarding the wear profile, surface roughness and depth of demineralization between the groups (p > 0.05).Conclusions. The chitosan gel reduced volume loss of the demineralized enamel without significantly impacting the surface smoothness.

show abstract

“…Additionally, the treatment was conducted on natural enamel surface without flattening to simulate the in vivo conditions [ 37 ]. Bovine enamel is readily available, and has found to have a close behavior to that of human enamel regarding biofilm cariogenicity and lesion severity [ 38 ]. However, bovine enamel has shown to have a higher hardness, larger crystal size, and faster rate of lesion progression (1.4:1) than those of human enamel [ 39 – 41 ].…”

Section: Discussionmentioning

confidence: 99%

Combined effects of a topical fluoride treatment and 445 nm laser irradiation of enamel against a demineralization challenge: A light and electron microscopic ex vivo study

2020

View full text Add to dashboard Cite

This study investigated the caries-preventive effect of 445 nm laser radiation in combination with fluoride on the prevention of white spot lesions. Previously, several studies have indicated the ability of 488 nm argon ion laser irradiation to reduce early enamel demineralization. A diode laser (445 nm) could be an alternative technology for possible cariespreventive potential. Each sample of a group of seventeen caries-free bovine teeth was treated in four different ways on four different zones of the labial surface: control/no treatment (C), laser irradiation only (L) (0.3 W, 60 s and applied dose of 90 J/cm 2), amine fluoride application only (10,000 ppm and pH 3.9) (F), and amine fluoride application followed by laser irradiation (FL). After treatment, the teeth were subjected to a demineralization solution (pH 4.3 for 48 h at 37˚C) to induce subsurface lesions. After sectioning, the teeth were examined by light microscopy. Three teeth were analyzed by scanning electron microscopy (SEM). The depths of the subsurface lesions in the C, L, F, and FL groups were 103.01 (± 13.04), 96.99 (± 14.51), 42.59 (± 17.13), and 24.35 (± 11.38) μm, respectively. The pairwise group comparison showed the following results: p < 0.001 for FL versus C, FL versus L, F versus C, and F versus L, p = 0.019 for FL versus F and p = 0.930 for L versus C. The SEM micrographs support the light-microscopic examination. The results of the current study have shown that using relatively low irradiation settings of 445 nm laser on fluoridated enamel may be effective for prevention of white spot lesions.

show abstract

Comparison of human and bovine enamel in a microbial caries model at different biofilm maturations

Cited by 16 publications

References 38 publications

Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study

Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study

Effect of an experimental chitosan/casein gel on demineralized enamel under a cariogenic challenge

Combined effects of a topical fluoride treatment and 445 nm laser irradiation of enamel against a demineralization challenge: A light and electron microscopic ex vivo study

Contact Info

Product

Resources

About